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Diss Factsheets
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EC number: 209-984-8 | CAS number: 600-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the hypersensitivity potential of alternative butter flavorings
- Author:
- Anderson, S.E., Franko, J., Wells, J.R., Lukomska, E., and Meade, B.J.
- Year:
- 2 013
- Bibliographic source:
- Food Chem Toxicol., 62: 373–381.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: Method described in the ICCVAM Peer Review Panel report
- Principles of method if other than guideline:
- Mice (5 per group) were exposed topically to the test chemical on the dorsal surface of each ear (25 ll per ear) for three consecutive days. On day 6, mice were injected intravenously 20 lCi 3H-thymidine and euthanized five hours later. The left and right auricular draining lymph nodes located at the bifurcation of the jugular vein were excised and pooled for each animal. Single cell suspensions were made and incubated overnight in 5% trichloroacetic acid and samples were counted using a Packard Tri-Carb 2500TR liquid scintillation analyzer.
Stimulation indices (SI) were calculated by dividing the mean disintegrations per minute (DPM) per test group by the mean DPM for the vehicle control group.
EC3 values (concentration of chemical required to induce a 3-fold increase over the vehicle control) were calculated based on the equation from Basketter et al. (1999).
Dosing concentrations (12.5–50%) were selected based on the results from the concentration range finding studies. The concentration of chemical required to induce a 3-fold increase over the vehicle control (EC3) was calculated based on the equations from Basketter et al. (1999). - GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Pentane-2,3-dione
- EC Number:
- 209-984-8
- EC Name:
- Pentane-2,3-dione
- Cas Number:
- 600-14-6
- Molecular formula:
- C5H8O2
- IUPAC Name:
- pentane-2,3-dione
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- 2,3-pentanedione, (97%, CAS# 600-14-6).
Sigma–Aldrich
Chemical Company, Inc. (Milwaukee, WI)
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Taconic (Germantown, NY)
- Females (if applicable) nulliparous and non-pregnant: not specified]
- Age at study initiation: 6-8 weeks
- Housing: 5 animals per cage in ventilated plastic showbox cages with hardwood chip bedding.
- Diet (e.g. ad libitum): ad libitum NIH-31 modified 6% irradiated rodent diet
- Water (e.g. ad libitum): ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 68-72
- Humidity (%): 36-57
Study design: in vivo (LLNA)
- Vehicle:
- other: Acetone
- Concentration:
- 0, 12.5, 25, 50 % v/v
- No. of animals per dose:
- 5 per group
- Positive control substance(s):
- other: DNCB 0.3 %
- Statistics:
- For analysis, data was first tested for homogeneity using the Bartlett’s Chi Square test. If homogeneous, a one-way analysis of variance (ANOVA) was conducted.
If the ANOVA showed significance at p < 0.05 or less, the Dunnett’s Multiple Range t test was used to compare treatment groups with the control group. Linear
trend analysis was performed to determine if the test articles had exposure concentration- related effects for the specified endpoints. Statistical analysis was performed using Graph Pad Prism version 5.0 (San Diego, CA). Statistical significance is designated by *(p 6 0.05) and **(p 6 0.01).
Results and discussion
In vivo (LLNA)
Results
- Key result
- Parameter:
- EC3
- Value:
- 17.9
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Conclusions:
- The test chemical tested positive in the LLNA with an EC3 value of 17.9%.
- Executive summary:
Based on an LLNA study in female mice, the test chemical is considered to be a category 1 sensitizer according to GHS criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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