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EC number: 500-046-6 | CAS number: 26183-52-8 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to the Category Approach Justification provided in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Source, WoE, 68439-50-9, Sasol, 1993, DK-566
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: recalculated LC50 on the basis of the content of 73% active substance contained in the product.
- Remarks:
- Source, WoE, 68439-50-9, Sasol, 1993, DK-566
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.91 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 0.7 - 1.2 mg/L
- Remarks:
- Source, WoE, 9002-92-0, Sasol, 1997, DK-962
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 2.1 - 2.9 mg/L
- Remarks:
- Source, WoE, 68439-46-3, Shell, 1985, SBGR.84.284
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 - 24 JUN 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: EG-Guideline 92/69/EWG
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Trade name: Marlipal 24/20
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: waterflea
- Strain: clone 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): approx. 24 h
- Feeding during test: none
ACCLIMATION
- Type and amount of food: Scenedesmus subspicatus ad libitum
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 +/- 1 °C
- pH:
- 7.4 - 7.5
- Dissolved oxygen:
- 7.1 - 8.4 mg/L
- Nominal and measured concentrations:
- nominal: 0.1, 0.2, 0.4, 0.8, 1.6 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: round bottom flasks filled with 10 mL test solution
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): no renewal - static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- Biomass loading rate: one test organism per 2 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: CaCl2 x 2 H2O: 294 mgL; MgSO4 x 7 H2O: 123 mg/L, NaHCO3: 63 mg/L and KCl: 5.5 mg/L
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: test start and test end
OTHER TEST CONDITIONS
- Photoperiod: darkness - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.61 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: recalculated EC50 on the basis of the content of 73% active substance contained in the product.
- Results with reference substance (positive control):
- The reference substance was tested in a separate test (1993-04-20)
- Mortality: 0.9 mg/L - 30%; 1.9 mg/L - 100% - Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-hour acute toxicity of alcohols, C12-14, ethoxylated (2 EO) to Daphnia magna, was determined in accordance with EU Guideline 92/69/EWG. The 48-hour EC50 value was determined to be 0.53 mg/L corresponding to 0.39 mg/L active ingredient. The test is considered valid.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 - 09 JAN 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: EG-Guideline 92/69/EWG
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: waterflea
- Strain: clone 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): approximately 24 h
- Method of breeding: parent animals were rared in M4-medium (according to Elendt, 1990) in 1 L beakers. Animals were fed daily with Scenedesmus subspicatus ad libitum: 24 h prior test, parent daphnids were separated. Juveniles hatching over night were used in the test.
- Feeding during test: none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 +/- 1 °C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 7.3 - 8.9 mg/L
- Nominal and measured concentrations:
- nominal concentrations: 1.5, 3.0, 6.0, 12 and 100 mg/L (waterfree substance)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: round bottom flasks
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): no renewal as static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: one test organism per 2 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: CaCl2 x 2 H2O: 294 mg/L; MgSO4 x 7 H2O: 123 mg/L; NaHCO3: 65 mg/L and KCl: 6.0 mg/L
- Ca/mg ratio: 4 : 1
OTHER TEST CONDITIONS
- Photoperiod: darkness - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.91 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? The reference substance was tested in a separate test (1996-10-08)
- Mortality: 1.0 mg/L: 14%; 2.0 mg/L: 100% - Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-hour acute toxicity of alcohols, C12, ethoxylated (4 EO) to Daphnia magna, was determined in accordance with EU Guideline 92/69/EWG. The 48-hour EC50 value was determined to be 0.91 mg/L. The test is considered valid.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 26 Oct - 28 Oct 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given (comparable to guidelines/standards)
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The acute toxicity to Daphnia magna was determined under static conditions.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (final test solution): 0.1 ml/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Laboratory culture obtained from ICI Brixham Laboratory; original source: I.R.Ch.A., France
- Age at study initiation (mean and range, SD): < 24 h
CULTURE
- Culture conditions: glass vessels (0.5 - 4 L) containing reconstituted fresh water. New cultures are started with animals < 24 h old, at a density of about 20 per litre. Cultures are held in a temperature controlled room at 18 – 22 °C and artificial light in a 16 h light/8 h dark cycle. The water in the vessels is renewed weekly and the cultures are discarded when they are 5 weeks old or if there appear ephippia.
- Type and amount of food: Chlorella vulgaris, approx. 1E06 cells/mL in the Daphnia vessels
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 160 mg/L as CaCO3
- Test temperature:
- 18 - 22 °C
- pH:
- 8.2 - 8.3
- Dissolved oxygen:
- 8.5 - 9.2 mg/L
- Nominal and measured concentrations:
- Nominal: 0.5, 1.1, 2.3, 4.8, 10, 22, 47, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Crystallising dishes
- Material, size, headspace, fill volume: glass, fill vol. 150 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per vehicle control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted fresh water containing 192 mg/L NaHCO3, 120 mg/L CaSO4*2H2O, 120 mg/L MgSO4, 8 mg/L KCl
- Aeration: Test water was aerated 24 h before use and was assumed to be saturated with dissolved oxygen
- Ca/Mg ratio: 1:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: Monitoring of temperature in one test vessel every 4 h, pH and dissolved oxygen was measured in one control and the highest test concentration at test start and test end.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilisation was recorded after 24 and 48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 2.0 - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL = 2.1 - 2.9 mg/L
Referenceopen allclose all
Nominal concentration |
Test period (h) |
|
24 h |
48 h |
|
Immobility [%] |
Immobility [%] |
|
Control |
0 |
0 |
0.1 |
0 |
0 |
0.2 |
0 |
0 |
0.4 |
10 |
20 |
0.8 |
75 |
95 |
1.6 |
100 |
100 |
Nominal concentration |
Test period (h) |
|
24 h |
48 h |
|
Immobility [%] |
Immobility [%] |
|
Control |
0 |
0 |
0.4 |
0 |
0 |
0.7 |
0 |
0 |
1.2 |
90 |
100 |
2.0 |
50 |
100 |
3.5 |
80 |
100 |
6 | 100 | 100 |
10 | 100 | 100 |
24 h EC50 value: 4.8 mg/L, 95% CL = 4.0 - 5.8 mg/L
Table: Immobilisation of D. magna
Concentration of test substance (mg/L) |
Number of D. magna |
Number immobilised |
|
24 h |
48 h |
||
Control |
10 |
0 |
0 |
Control |
10 |
0 |
0 |
Control |
10 |
0 |
0 |
0.5 |
10 |
0 |
0 |
0.5 |
10 |
0 |
0 |
0.5 |
10 |
0 |
0 |
1.1 |
10 |
0 |
2 |
1.1 |
10 |
0 |
0 |
1.1 |
10 |
0 |
0 |
2.3 |
10 |
1 |
3 |
2.3 |
10 |
2 |
4 |
2.3 |
10 |
1 |
5 |
4.8 |
10 |
4 |
8 |
4.8 |
10 |
6 |
10 |
4.8 |
10 |
6 |
10 |
10 |
10 |
9 |
10 |
10 |
10 |
8 |
10 |
10 |
10 |
8 |
10 |
22 |
10 |
10 |
10 |
22 |
10 |
10 |
10 |
22 |
10 |
10 |
10 |
47 |
10 |
10 |
10 |
47 |
10 |
10 |
10 |
47 |
10 |
10 |
10 |
100 |
10 |
10 |
10 |
100 |
10 |
10 |
10 |
100 |
10 |
10 |
10 |
Description of key information
EC50 (48h) = 0.39 mg/L for Daphnia magna
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.39 mg/L
Additional information
Since no data for decan-1-ol, ethoxylated, 1-2.5EO (CAS 26183-52-8) are available the acute toxicity on aquatic invertebrates is assessed on the basis of data from studies with three similar alcohol ethoxylates (C9-11, 2.5EO; CAS 68439-46-3, C12, 4 EO; CAS 9002-92-0 and C12-14, 2 EO; CAS 68439-50-9).
For all three analogue substances the immobilisation of the water flea Daphnia magna was investigated (Sasol 1993 & 1997 and Shell 1985). Two tests were performed according to EG-Guideline 92/69/EWG and GLP (Sasol, 1993 & 1997), whereas one study followed no defined guideline and was not performed under GLP conditions (Shell, 1985). The evaluated EC50 (48h) of the three studies are in a narrow range between 0.39 mg/L and 2.5 mg/L. The lowest value of 0.39 mg/L is used as worst case key value for the further assessment.
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