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Diss Factsheets
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EC number: 246-186-9 | CAS number: 24347-58-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 July 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- guideline version from 1993
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1993
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch no. 34-2127
Date of production: December 1998
Colour: colourless/yellowish - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/l dry substance and aerated for about 24 hours. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/l dry substance in the test.
- Inoculum: concentration of dry substance: 1 g/l
- Pretreatment: none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Test temperature:
- 20±2 °C
- pH:
- 7.4 (start) - 7.7 (end)
- Dissolved oxygen:
- 7.9 (start) - 6.5 (after 6 min) mg/L O2/L
- Nominal and measured concentrations:
- Nominal test concentration: 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Type (delete if not applicable): closed
- Aeration: none
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No respiration inhibition observed.
- Results with reference substance (positive control):
- EC50 ca. 23 mg/L (3,5-dichiorophenol)
- Validity criteria fulfilled:
- yes
- Remarks:
- according to OECD 209 adopted 1993
- Conclusions:
- The test substance did not inhibit respiration of activated sludge microorganisms at 1000 mg/L. The derived 30min - EC50 (sludge microorganisms, respiration inhibition) > 1000 mg/L (nominal).
- Executive summary:
The study investigated respiration inhibition of activated sludge microorganisms according to OECD guideline 209 (adopted 1993). Domestic activated sludge was used as inoculum. No inhibition of respiration was observed under static test conditions. The effect concentrations EC20 and EC50 (respiration inhibition) were derived at > 1000 mg/L after 30 minutes of exposure. In conclusion, the test substance was considered to not inhibit respiration of activated sludge microorganisms. The validity criteria of the test guideline (adopted 1993) were fulfilled. Despite of some deficiencies with respect to the updated guideline, the study was considered adequate and reliable for environmental hazard assessment.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please find endpoint specific justification in the read-across assessment report.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- assessment report
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: Effect conc. are identical for the submission substance, because target and source substance have the same MW.
- Validity criteria fulfilled:
- yes
- Remarks:
- according to OECD 209, adopted 1993
- Conclusions:
- The test substance did not inhibit respiration of activated sludge microorganisms at 1000 mg/L. The derived 30min - EC50 (sludge microorganisms, respiration inhibition) > 1000 mg/L (nominal).
- Executive summary:
The study used as source investigated repiration inhibition of actived sludge microorganisms. The study results of the source compound were considered applicable to the target compound. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13.
Referenceopen allclose all
MEASURED DATA blank control (BC) and test substance (TS) after 30 minutes incubation time
Vessel no.: | BC1 | BC2 | BC3 | my BC |
TS1 |
Concentration of test substance (mg/1) | - | - | - | - | 1000 |
Added volume of stock solution(mVvessel) | - | - | - | - | - |
Direct addition (mg/vessel) | - | - | - | - | 250.0 |
Oxygen concentration start (mg 02/I) | 8.3 | 8.3 | 8.4 | - | 7.9 |
Oxygen concentration after 6 min (mg 02/l) | 7.0 | 7.0 | 7.2 | - | 6.5 |
Oxygen consumption rate (mg02/I*6 min) | 1.3 | 1.3 | 1.2 | 1.3 | 1.4 |
Oxygen consumption rate (mg02/1*h) | 13 | 13 | 12 | 13 | 14 |
Specific oxygen consumption rate (mg02/g*h) | 13 | 13 | 12 | 13 | 14 |
Change in oxygen consumption ratecompared with the blank control (%) | - | - | - | - | -8 |
my BC = mean value of blank control
pH-VALUES blank control (BC) and test substance (TS) after 30 minutes incubation time
Vessel no.: | BC1 | BC2 | BC3 | TS1 | |
pH-value before adding the inoculum before correction: |
6.7 | 6.7 | 6.7 | 6.7 | |
after correction: | 7.5 | 7.2 | 7.4 | 7.4 | |
pH-value after an incubation time of 30 min | - | - | - | 7.7 |
Description of key information
EC50 (sludge microorganisms, respiration) > 1000 mg/L (experimental, OECD 209)
Key value for chemical safety assessment
Additional information
No toxicity data concerning respiration inhibition of activated sludge is available for the submission substance. However, adequate and reliable data are reported here for a structural analogue (source substance 2,3 -butanediol). Please also refer to the read-across justification document.
In the reliable key study respiration inhibition of activated sludge microorganisms was investigated according to OECD guideline 209 (adopted 1993). Domestic activated sludge was used as inoculum. No inhibition of respiration was observed under static test conditions. The effect concentrations EC20 and EC50 (respiration inhibition) were derived at > 1000 mg/L after 30 minutes of exposure. In conclusion, the test substance was considered to not inhibit respiration of activated sludge microorganisms. The validity criteria of the test guideline (adopted 1993) were fulfilled. Despite of some deficiencies with respect to the updated guideline, the study was considered adequate and reliable for environmental hazard assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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