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Diss Factsheets
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EC number: 467-100-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 February 2018 - 03 May 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- After 0h; 24h and 48h exposure, all replicates of control and treatment groups were sampled (5 ml), one sample per replicate, 4 samples per treatment group and control
Of each sampled treatment, one of the analytical samples from 0h, 24h and 48h was sent to the analytical laboratory at the test site for chemical analysis. The remaining samples were stored as retain samples in the freezer (<=18°C) until finalization of the study. - Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared as Water Soluble Fraction (WSF) by adding 400 mg test item to 1000 ml test medium and shaking for 24 h using an overhead shaker at 20.3 - 21.5 °C. The stock solution was allowed to settle for 1.5 h before decanting the upper part of the liquid column for use in the test. This decanted stock solution was used as highest tst item loading rate in the test. The negative control (test medium) was treated in the same way as the test item solution.
4 replicates, with 5 daphnids each, were prepared for each test item treatment and the negative control. The further test item loading rates were prepared by diluting the stock solution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flee
- Source: federal environmental Agency, Berlin
- test organisms used were 0.17 - 21.75 h old at the start of the test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- the test was performed with Elendt M4 medium according to OECD 202 (2004)
- Test temperature:
- 20.1-21.1
- pH:
- control: 7.5-7.9
test item treatment: 7.6-9.9 - Dissolved oxygen:
- conrol: 8.2-8.3
test item treatment: 8.0-8.2 - Nominal and measured concentrations:
- nominal: 0; 25; 50; 100; 200; 400 mg/l
measured (geometrical mean): 0; 22.68; 43.85; 84.83; 170.63; 305.25 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml glass beakers
- Type (delete if not applicable): open
- fill volume: 40 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light, 8h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : the swimming capability of the daphnids was determined after 24h and 48h exposure. pH and oxygen concentration was measured after 0h; 24h, and 48h, the test vessels were kept in an icubator with constant temperature
RANGE-FINDING STUDY
- Test concentrations: 50; 100; 200 mg/l
- Results used to determine the conditions for the definitive study: no immobility in each concentration was observed after 48h - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 265.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 170 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 84.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 = 265.7 mg/l
- Executive summary:
In a valid Daphnia magna acute immobilisation test according to OECD 202 under GLP conditions, the calculated EC50 = 265.7 mg/l based on analytically measured test item concentration (geometric mean concentration).
Reference
Nominal test item loading rate (mg/l) |
Daphnia introduced |
Immobile Daphnia (replicates 1-4) |
Immobility(%) |
|||||||||||
0 h |
24 h |
48 h |
||||||||||||
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
24 h |
48 h |
|
control |
5 |
5 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
25 |
5 |
5 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
50 |
5 |
5 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
5 |
5 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
200 |
5 |
5 |
5 |
5 |
2 |
0 |
0 |
1 |
3 |
0 |
0 |
1 |
15 |
20 |
400 |
5 |
5 |
5 |
5 |
1 |
0 |
1 |
2 |
2 |
2 |
4 |
4 |
20 |
20 |
Description of key information
In a valid Daphnia magna acute immobilisation test according to OECD 202 under GLP conditions, the calculated EC50 = 265.7 mg/l based on analytically measured test item concentration (geometric mean concentration).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 265.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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