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Diss Factsheets
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EC number: 936-618-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Exp. part: Feb 27 – Oct 25, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The samples were taken from the biological phase of the study. Collecting, storage and handing over of the samples were the Study Director’s responsibility. The information concerning the samples was provided by the Study Director.
One sample of the filtrate of the stock solution and duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test and at day 1.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the aged test media of all test concentrations and the control were collected at day 1 (after 24 hours of exposure) and at the end of the test by pouring together the contents of the test beakers of each treatment.
All samples were diluted by a factor of two with acetonitrile.
Additional samples of the control and the dilution solvent were taken at each sampling without any sample treatment. - Vehicle:
- no
- Details on test solutions:
- The test item is not well soluble in test medium. To avoid physical effects of undissolved test item on the daphnids, no concentrations above the solubility limit of the test item in test water was tested. Therefore, a supersaturated stock solution of nominal 100 mg test item/L was prepared by suspending 50 and 55.8 mg test item in 500 and 558 mL test water. The stock suspension was stirred for 48h hours at room temperature in the dark to dissolve as much test item as possible. Then, non-dissolved fractions of the test item were separated from the test medium by membrane filtration (0.45 μm cellulose acetate filter). The solution with dissolved test item was used to prepare the desired 1:8, 1:16, 1:32, 1:64 and1:128 dilutions.
The test media were prepared just before introduction of the daphnids (= start of the test) and test medium renewal on Day 1. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Strain/Clone: Daphnia magna, clone 5;
Age: 1.5 to 19.25 hours old
Source: The daphnids introduced in the test were taken from in-house laboratory culture. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L
- Test temperature:
- 18.3 to 19.7 °C in the freshly prepared media; 18.7 to 19.2°C in the aged test media
- pH:
- 7.9 to 8.1 in the freshly prepared media; 7.8 to 7.9 in the aged test media
- Dissolved oxygen:
- 9.3 to 10.0 mg/L in the freshly prepared media; 8.7 to 9.9 mg/L in the aged test media
- Nominal and measured concentrations:
- Due to the low limit of water solubility of the test item, a filtrate of nominal 100 mg/L was produced. Afterwards dilutions of this filtrate (1:8/ 1:16 / 1:32/ 1:64 and 1:128) resulting in nominal concentrations of 12.5, 6.3, 3.1 and 1.6 and 0.8 mg test item/L were tested.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11.151 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: based on nominal loading rate
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.33 µg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: based on measured concentration
- Details on results:
- After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the nominal loading rate of 6.30 mg test item/L. At the loading rate of 12.50 mg test item/L, 12 animals were immobile after 48 h of exposure.
The 48 h EC50 was determined to be 11.151 mg/L based on a nominal loeading rate. This is corresponding to 3.33 µg/L test material concentration. - Results with reference substance (positive control):
- EC50 [mg/L]: 1.58 (24h) and 1.01 (48 h)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic effect of the test item to Daphnia magna was assessed in a semi-static concentration-response test. The 48 h EC50 was determined to be 11.151 mg/L based on a nominal loeading rate. This is corresponding to 3.33 µg/L test material concentration.
The initial concentrations and the maintenance of the exposure concentrations during the test were determined in the analytical part. All reported results refer to nominal loading rates since the concentrations of the test samples could not be quantified. - Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- For this endpoint information from structural similar compounds is available. The studies for these similar compounds were performed according to GLP and the methods applied are fully compliant with OECD TG 202. See chapter 13 report for a more detailed justification.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11.51 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: based on loading rate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.33 µg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: based on measured values
- Conclusions:
- For this endpoint a one-to-one read across was performed to a chemical similar compound of the same chemical class with a comparable phys. chem. profile and similar response in biological assays. The relevant study was performed according to GLP and the methods applied are fully compliant with OECD TG 202. The read across results shows an EC50 (48 h) of 11.51 mg/L (nominal concentration) which corresponds to 3.33 µg/L (calculated based on measured concentration). A detailed read across justification is provided in chapter 13 of this dossier.
Referenceopen allclose all
Description of key information
No data for short-term toxicity to aquatic invertebrates are available for the test item, however, a Guideline study under GLP conditions was conducted with a structural analogue substance to cover the endpoint adequately.
In the OECD 202 study, the EC50 (48 h) was calculated to be at 11.115 mg/L based on the nominal loading rate. Using the measured concentration of 30 µg/L for the stock solution (100 mg/L) and considering a dilution factor of 9, the nominal concentration of 11.115 mg/L corresponds to a measured concentration 3.33 µg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3.33 µg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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