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EC number: 306-529-6 | CAS number: 97281-29-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 22, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- Respirometer
- Details on sampling:
- - Measurement for each group, i.e. test substance, negative and positive controls
- After 10 min - Vehicle:
- no
- Details on test solutions:
- Stock solution: 1.0 mg/mL in water and the appropriate amount was directly added in the test solution
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Source: Warcham Waste Water Treatment Plant, Warchal, Massachussets
Solid contents: 4.1 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- coninuously aerated
- Test temperature:
- 20.6 - 20.8
- pH:
- 6.88 -7.27
- Salinity:
- -
- Conductivity:
- -
- Nominal and measured concentrations:
- 0 (negative control), 0.1, 1.0, 10, 100 and 1000 mg/L
- Details on test conditions:
- Final volume of 500 mL
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Control vessels: 15.9 and 16.8 mg O2/L/hour.
Test substance vessels at concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L: 17.2, 15.3, 18.0, 16.4 and 18.5 mg O2/L/hour, respectively
No differences were observed between the control and the test conditions - Results with reference substance (positive control):
- Positive control vessels at concentrations of 1.0, 3.0, 10 and 30 mg/L: 10.8, 11.0, 5.7 and 1.8 mg O2/L/hour, respectively
EC50 value of 5.7 mg a.i./L - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 3 h EC50 (in activated sludge) of the test substance was determined to be >1000 mg/L (nominal).
- Executive summary:
A study was conducted to determine the toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to microorganisms according to OECD Guideline 209 (Activated Sludge Respiration Inhibition Test), in compliance with GLP. Approximately 4 g/L of activated sludge (domestic) was exposed to the test substance at concentrations of 0 (negative control), 0.1, 1.0, 10, 100 and 1000 mg/L for 3 h. The dissolved oxygen (measurement of oxygen depletion rates or oxygen consumption) was recorded using a respirometer. A positive control group (3,5 -dichlorophenol) was included in the experiment and gave values within the expected range. In the control vessels, the oxygen consumption was 15.9 and 16.8 mg O2/L/h and, in the test substance vessels at concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L, it was 17.2, 15.3, 18.0, 16.4 and 18.5 mg O2/L/h, respectively. No statistical differences were observed between the negative controls and the test groups. Under the study conditions, the 3 h EC50 (in activated sludge) of the test substance was determined to be >1000 mg/L (Springborn, 2010). No NOEC was reported by the author, however considering the absence of toxicity at any dose level, the NOEC can be assumed to be 1000 mg/L (nominal).
Reference
Description of key information
Based on the results of the study, 3 h EC50 (in activated sludge) of the test substance, 'di-C18-22 AAEMIM-MS', to microorganisms, was determined to be >1000 mg/L (nominal).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
A study was conducted to determine the toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to microorganisms according to OECD Guideline 209 (Activated Sludge Respiration Inhibition Test), in compliance with GLP. Approximately 4 g/L of activated sludge (domestic) was exposed to the test substance at concentrations of 0 (negative control), 0.1, 1.0, 10, 100 and 1000 mg/L for 3 h. The dissolved oxygen (measurement of oxygen depletion rates or oxygen consumption) was recorded using a respirometer. A positive control group (3,5 -dichlorophenol) was included in the experiment and gave values within the expected range. In the control vessels, the oxygen consumption was 15.9 and 16.8 mg O2/L/h and, in the test substance vessels at concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L, it was 17.2, 15.3, 18.0, 16.4 and 18.5 mg O2/L/h, respectively. No statistical differences were observed between the negative controls and the test groups. Under the study conditions, the 3 h EC50 (in activated sludge) of the test substance was determined to be >1000 mg/L (Springborn, 2010)
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