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EC number: 203-770-8
CAS number: 463-04-7
The objective of this study was to assess the aerobic ready
biodegradability of test item by measurement of manometric respirometry
over a 28-day period according to the OECD 301F guideline (July 1992).
The test item, at approximately 100 mg/L, as the sole source of carbon
and energy, was incubated in a buffer-mineral salts medium which was
inoculated with a mixed population of micro-organisms. The consumption
of oxygen was determined from the change in pressure in the apparatus.
Evolved carbon dioxide was absorbed in a solution of sodium hydroxide.
The amount of oxygen taken up by the microbial population during
biodegradation of the test substance (corrected for uptake by blank
inoculum, run in parallel) was expressed as a percentage of ThODNH3.
As the test item is poorly soluble in test conditions and volatile, the
test solutions were prepared by direct addition of the test item on the
The study is valid since:
Based on a biodegradation rate higher than 60% in 14 days (experimental
value: 78.9 and 69.1 % in 6 days) in the reference control, the test was
carried out under good conditions.
At the end of the test, measurements of Biological Oxygen Demand (BOD)
in two replicates of the test item solution did not show a difference of
more than 20% (experimental value: 8.3 %).
The oxygen uptake of the control was not greater than 60 mg/l in 28 days
(experimental value: 33.1 and 27.0 mg O2/L).
Based on a biodegradation rate higher than 25% in 14 days (experimental
value: 97.4 % in 13 days) in the toxicity control, the test item was not
toxic to micro-organisms.
Results are the following:
Percentage of biodegradation at 28 days : 95.7 %
Conclusion : Readily biodegradable
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