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EC number: 458-680-3 | CAS number: 797751-44-1 WASOX-VMAC2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 22-24, 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- According to EU Method C.2, with GLP.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Crustacea, Cladocera
- Source: Daphtoxkit F (Daphnia magna; MicroBioTests Inc., Industriezone "De Prijkels", Venecoweg 19 , 9810 Nazareth, Belgium).
- Age at study initiation (mean and range, SD): less then 24 hours old
- Feeding during test: no
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- 20 ºC throughout the test period
- pH:
- Between 7.6 and 7.9 in the test substance medium
- Dissolved oxygen:
- The dissolved oxygen concentration was 9.3-9.5 mg/L in the test substance medium (9.6-9.8 mg/L in control) .
- Nominal and measured concentrations:
- 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
Disposable multiwell test plates with 30 test wells with a transparent lid, provided with the testkit. Each plate has four
10 mL wells each (replicates A,B,C,D) for the control and for 5 concentrations. Additionally, for each concentration the plates are provided with "rinsing wells" to prevent dilution of the test substance during the transfer of the daphnia from the hatching petri dish to the test wells .
- Aeration: No extra aeration, but slight circulation of air is still possible when the lid of the test plate is closed.
OTHER TEST CONDITIONS
- Photoperiod: artificial light from 4 a.m. to 8 p.m.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobilization:
24 and 48 hours after start of exposure to the test substance the neonates were observed for their swimming behavior. Animals not able to swim within approximately 15 s after gentle agitation of the test container are evaluated as immobilized.
Additionally immobilized animals trapped at the surface were counted to evaluate a possible influence of the test substance to the water surface tension. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
- Mortality of control: there was no immobilization in the negative control group
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: the test media were clear and colorless during the whole exposure time - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 value of the test substance, based on the nominal concentration of test substance equivalents at the start of the assay, was >100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.
- Executive summary:
The Acute Toxicity of the test substance to Daphnia magna was determined in a 48 hours static test (According to EU Method C.2). A total of 20 daphnias, divided into 4 replicates (5 daphnias each) were treated with a single dose of 100 mg/L (Limit test). A negative control group, exposed to reconstituted water only, was included. Daphnia immobilization was inspected at 24h and 48 h after the start of the test, as well as immobilized animals trapped at the surface were counted to evaluate a possible influence of the test substance to the water surface tension. The pH and the dissolved oxygen concentration were tested at the start and at the end of the test. The temperature was controlled at the start, after 24 hours and at the end of the test. Test validity criteria were fulfilled.
The 24 and 48 hours EC50 value of the test substance was > 100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.
Reference
Immobilization of neonates.
The nominal concentration of test substance equivalents is displayed.
The concentrations are presented in units of mg test substance equivalents / L to indicate that not the test substance but a variety of degradation products was tested.
Immobilization 24 hours after the start of exposure
|
T.s. |
Immobilised neonates / total number of neonates |
|||||
Group |
concentration |
Number per replicate |
Total |
Total |
|||
|
(mg/L) |
A |
B |
C |
D |
number |
% |
Control |
0 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
0 |
T.s. |
100 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
0 |
Immobilization 48 hours after the start of exposure
|
T.s. |
Immobilised neonates / total number of neonates |
|||||
Group |
concentration |
Number per replicate |
Total |
Total |
|||
|
(mg/L) |
A |
B |
C |
D |
number |
% |
Control |
0 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
0 |
T.s. |
100 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
0 |
T.s. test substance
Description of key information
The Acute Toxicity of the test substance to Daphnia magna was determined in a 48 hours static test (According to EU Method C.2). The 24 and 48 hours EC50 value of the test substance was > 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
A total of 20 daphnias, divided into 4 replicates (5 daphnias each) were treated with a single dose of 100 mg/L (Limit test). Daphnia immobilization was inspected at 24h and 48 h after the start of the test, as well as immobilized animals trapped at the surface were counted to evaluate a possible influence of the test substance to the water surface tension. The temperature was controlled at the start, after 24 hours and at the end of the test.Test validity criteria were fulfilled.
The 24 and 48 hours EC50 value of the test substance was > 100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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