Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 412-960-1 | CAS number: 148878-21-1 PROCION BRILLIANT ORANGE H-EXL; S156745
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Septemeber 27, 1993 to September 29, 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Purity: 66.9% w/w
Batch number: NBY 35071/15
Physical state: solid
Color: orange/red - Analytical monitoring:
- yes
- Details on sampling:
- The concentration of test substance in the test solutions was measured at the start and end of the test using the method. At the start of the test the remaining excess of the test solutions was sampled and analysed. At the end of the test each of the replicates of the test solutions were sampled and analysed.
- Vehicle:
- no
- Details on test solutions:
- A nominal 180 mg/L solution of the test substance was prepared by dissolving 0.1806 g of test substance in 1 L of dilution medium. The resultant solution was clear and bright orange in color.
The following nominal exposure concentrations were employed:
Control and 180 mg/l. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Fresh water crustacean
- Strain/clone: Daphnia magna
- Food type: Chlorella vulgaris and "Frippak Booster" (a commercially available microencapsulated diet)
Conditions:
-Temperature: 20 ± 2°C
-Photoperiod: 16 h light/ 8 h dark
The Daphnia were not fed during the course of the test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 ± 1°C
- pH:
- 8.38 to 8.48
- Dissolved oxygen:
- 8.8 to 9.0 mg/L
- Nominal and measured concentrations:
- The mean measured concentration of test substance was 106% of the nominal value of 180 mg/L.
- Details on test conditions:
- Borosilicate glass beakers of 250 mL capacity were used as test vessels, with four replicates per exposure concentration. Each vessel contained 200 mL of test solution providing a depth of approximately 60 mm. The beakers were covered with loose fitting lids. The test was initiated by adding five Daphnia to each vessel, in <2 mL of dilution water, giving a total of 20 Daphnia per concentration. The test solution temperature was maintained at 20 ± 1°C by control of the room temperature and a photoperiod of 16 hours light and 8 hours dark, with 15 minute transition periods, was provided. The test solutions were not aerated.
The concentrations of analytical grade chemicals used to prepare the medium in glass-distillated water were as follows:
NaHCO3 (mg/L) 192
CaSO4.2H20 (mg/L) 120
MgSO4.7H20 (mg/L) 245
KCl (mg/L) 8 - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 180 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The numbers of Daphnia immobilized at each concentration, after 24 and 48 hours, are provided in table in other information on results.
The EC50 values obtained were:
24 hours >180 mg/L
48 hours >180 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the mean measured 48 h EC50 of the substance in Daphnia magna was determined to be greater than 180 mg/L.
- Executive summary:
A study was conducted to determine the acute toxicity of the substance to Daphnia magna according to OECD Guideline 202 and EU method C.2, in compliance with GLP. 20 daphnids were exposed to the test substance at a nominal concentration of 180 mg/L for 48 h under static conditions. Immobility was determined at 24 and 48 h. Analytical dose verification was conducted. Under the study conditions, the mean measured 48 h EC50 of the substance in Daphnia magna was determined to be greater than 180 mg/L (Comber, 1993).
Reference
Acute toxicity to Daphnia magna
Time (h) | Nominal concentration (mg/L) | Number immobilised per replicate | Total number tested | Total number immobilised | % immoboilised | |||
A | B | C | D | |||||
24 | control | 0 | 0 | 0 | 0 | 20 | 0 | 0 |
180 | 0 | 0 | 0 | 0 | 20 | 0 | 0 | |
48 | control | 0 | 0 | 0 | 0 | 20 | 0 | 0 |
180 | 0 | 0 | 0 | 0 | 20 | 0 | 0 |
Acute toxicity to Daphnia magna: analytical results
Nominal concentration (mg/L) | Measured concentration (mg/L) | Mean measured concentration over the test duratrion (mg/L) | Mean measured concetration as % of nominal * | |
0 hour | 48 hours | |||
Control | <0.42 | <0.42 | <0.42 | - |
180 | 180 | 190 | 190 | 106% |
* Mean of individual determinations on each replicate test solution at the end of the test; the individual values were 180, 180, 190 and 190 mg/L respectively.
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 180 mg/L
Additional information
A study was conducted to determine the acute toxicity of the substance to Daphnia magna according to OECD Guideline 202 and EU method C.2, in compliance with GLP. 20 daphnids were exposed to the test substance at a nominal concentration of 180 mg/L for48 h under static conditions. Immobility was determined at 24 and 48 h. Analytical dose verification was conducted.Under the study conditions, the mean measured 48 h EC50 of the substance in Daphnia magna was determined to be greater than 180 mg/L (Comber, 1993).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.