Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 236-526-4 | CAS number: 13419-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From July 10, 1990 to August 07, 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: E.E.C.: Directive 84/449
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Ammonium 2-mercaptopropionate
- EC Number:
- 236-526-4
- EC Name:
- Ammonium 2-mercaptopropionate
- Cas Number:
- 13419-67-5
- Molecular formula:
- C3H9NO2S
- IUPAC Name:
- ammonium 2-sulfanylpropanoate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Species, strain: Ico rat OFA.SD. (IOPS Caw),
Supplier: Iffa-Credo (69592 L'Arbresle Cedex - France),
Age: young adults between 5 and 7 weeks old.
Weight at the start of treatment (main study): from 117 g to 160 g (the individual weighs for each sex varied by no more than 20 % of the mean weight of the animals).
Environmental conditions:
Cages housed by sex and in groups of 5 (or 2 for the preliminary study), in type FI polycarbonate cages (interior dimensions 305 x 180 x 184 mm) for the preliminary study and in type MI (interior dimensions 365 x 225 x 180 mm) for the main study.
Air changes: at least 10 per hour
Temperature: 22 ± 3 °C
Humidity: 30 to 70 %
Lighting: artificial, 12 hours out of 24.
Hygiene: bedding changed once a week; cages changed for each study.
Diet: complete pelleted rat-mouse maintenance diet ad libitum (U.A.R., formula A.04 CR - U.A.R.).
Water: softened and filtered (0.6 µm) mains drinking water ad libitum. Bacteriological and chemical analyses checked twice a year.
Acclimatisation period: 8 days before the start of treatment.
Clinical examinations: at reception then before the start of treatment to ensure that only healthy animals are included in the study.
Identification of animals: perforation of ear pinna before the start of treatment.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- Single oral administration by gestric gavage - round –ended curved stainless-steel oesophageal probe, 3 mm diameter, Perfektum.
- Doses:
- Preliminary test: 499, 998 and 2007 mg/kg
Main test: 1125, 1415, 1601 and 1786 mg/kg - No. of animals per sex per dose:
- Preliminary study: 6 males, 6 non-pregnant females;
Main study:
control group - 5 males, 5 non-pregnant females;
groups 2 to 5 (treated) - 20 males, 20 non-pregnant females. - Control animals:
- yes
- Details on study design:
- Preliminary test:
3 groups each composed of 2 males and 2 females were treated, at dose levels of 499, 998 and 2007 mg/kg respectively at a volume of 0.43, 0.86 and 1.73 ml/kg of test substance as supplied. The test substance was administered after about 18 h of a water regime and received food 4 h after intubation.
Main test:
Five groups of 5 females and 5 males were dosed with at the levels of 1125, 1415, 1601 and 1786 mg/kg, corresponding to 0.97, 1.22, 1.38 and 1.54 ml/kg respectively. The control group was treated with the distilled water under the same conditions. The test article was administered once only after about 17 h of a water regime and received food 4 h after intubation.
Following examinations were performed:
- Clinical examinations: 15 minutes after intubation, then at 1, 2 and 4 hours and then daily for 14 days.
The daily observations performed, amongst others, included changes in the skin and fur, the eyes, mucous membranes, respiratory system, circulatory system, autonomic and central nervous systems, as well as somato-motor activity and behaviour. Shivering, convulsions, salivation, diarrhoea, lethargy, sleeping and coma were noted with particular attention.
- Observation of body weight: Day -1, Days 1 (before administration of the test article), 8 and 15, as well as at the time of death from Day 2 onwards.
- Necropsy examination of all animals. The abdominal and thoracic cavities were opened and particular attention was paid to the following organs: liver, heart, kidneys and lungs.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 518 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 1 435 - < 1 606
- Mortality:
- 20 % in 1415 mg/kg dose level; 80 % in 1601 mg/kg dose level; 90 % in 1786 dose level.
- Clinical signs:
- other: At all tested doses: all animals showed subdued behaviour 15 min, 1, 2 and 4 h after administration of the test substance. At 1125 mg/kg: all surviving animals were normal on the day following treatment. At 1415 mg/kg: one rat showed tremors at 1 h. All
- Gross pathology:
- Animals which died during the study showed congested area in the lungs. No macroscopic determinable abnormality was noted in animals killed on study termination.
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- Under the study conditions, the LD50 of the test substance in the rat (male and female) was determined to be 1518 mg/kg bw (1435-1606) by the Bliss method and 1522 mg/kg bw (1421-1630) by the Litchfield & Wilcoxon’s method.
- Executive summary:
A study was conducted to determine the potential toxic effect of the test substance when administered as a single oral dose to Wistar rats according to OECD Guideline 401 and EC Directive 84/449. Five groups of 5 females and 5 males were dosed with the test substance at the levels of 1125, 1415, 1601 and 1786 mg/kg. The control group was treated with the distilled water under the same conditions. Mortality and abnormal clinical signs were noted 15 minutes after intubation, then at 1, 2 and 4 h, and then daily for the 14 d study period. All the animals were weighed the day before treatment (Day -1), immediately before administration of the test substance (Day 1), on Days 8 and 15, as well as at time of death from Day 2 onwards. A necropsy was performed for all the animals dead during the study and for all surviving rats after the 14 d study period and the final observation (Day 15). Under the study conditions, the LD50 of the test substance in the rat (male and female) was determined to be 1518 mg/kg bw (1435-1606) by the Bliss method and 1522 mg/kg bw (1421-1630) by the Litchfield & Wilcoxon’s method (Lheritier, 1990).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.