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Diss Factsheets
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EC number: 220-278-9 | CAS number: 2698-41-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- 2-Chlorobenzylmercapturic Acid, A Metabolite of the Riot Control Agent 2-Chlorobenzylidene Malononitrile (CS) in the Rat
- Author:
- E. C. Rietveld x, L. P. C. Delbressine, T. H. J. M.Waegemaekers, and F. Seutter-Berlage
- Year:
- 1 983
- Bibliographic source:
- Arch Toxicol (1983) 54:139-144
- Reference Type:
- review article or handbook
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- The Salmonella/microsome assay for mutagenicity was performed with salmonella Typhimurium TA 100, according to Ames et al. (1975).
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- [(2-chlorophenyl)methylene]malononitrile
- EC Number:
- 220-278-9
- EC Name:
- [(2-chlorophenyl)methylene]malononitrile
- Cas Number:
- 2698-41-1
- Molecular formula:
- C10H5ClN2
- IUPAC Name:
- [(2-chlorophenyl)methylene]malononitrile
- Reference substance name:
- 1,1,1,3,3,3-hexamethyldisilazane
- EC Number:
- 213-668-5
- EC Name:
- 1,1,1,3,3,3-hexamethyldisilazane
- Cas Number:
- 999-97-3
- Molecular formula:
- C6H19NSi2
- IUPAC Name:
- 1,1,1-trimethyl-N-(trimethylsilyl)silanamine
- Reference substance name:
- Cab-0-Sil@ colloidal silica
- IUPAC Name:
- Cab-0-Sil@ colloidal silica
Constituent 1
Constituent 2
Constituent 3
Method
- Target gene:
- Not specified
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 100
- Test concentrations with justification for top dose:
- Test without metabolic activation : 5 concentrations : between 0 to 2000 µg/plate
Test with metabolic activation : several concentrations between 0 to 3500 µg/plate - Vehicle / solvent:
- Not specified
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- sodium azide : 1.0 µg or 2.5 µg/plate - benzoapyrène : 5.0 µg/plate
- Positive control substance:
- sodium azide
- benzo(a)pyrene
- Details on test system and experimental conditions:
- The assays were performed without pre-incubation.
- Rationale for test conditions:
- Not specified
- Evaluation criteria:
- Not specified
- Statistics:
- Not specified
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 1000 µg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- With rat liver homogenate the bacteriotoxicity of CS was slightly stronger than without it.
Applicant's summary and conclusion
- Conclusions:
- No mutagenic effect was detected using CS either with or without rat liver homogenate (S-9 mix)
- Executive summary:
In a study which methodology is comparable to OECD 471 (Ames test), no mutagenic effect was detected using CS either with or without rat liver homogenate (S-9 mix)
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