Phenol, para-alkylation products with C10-15 branched olefins (C12 rich) derived from propene oligomerization, reaction products with sulphur monochloride and dec-1-ene

Short-term toxicity to fish

Description of key information

There are a number of studies for this endpoint but the most robust of these give the same result; the LL50 for the registration substance is therefore expected to be the same due to the structural similarities.  Test results reported in the study as “lethal concentrations” are reported in this summary as “lethal loading rate”, because test results are based on WAF loading rates.
However, the registration material does not contain oil as a component, hence the results of the tested samples (their LC50s/LL50s) are adjusted to account for the % of active ingredient present in order to create LC/LL50 values for the registration material. 
Freshwater results: Nominal concentrations including oil: 96-h LL50 (reported as “LC50” in the report) >1,000 mg/L. This is equivalent to 96-h LL0 (reported as “NOEC” in the report) of 1,000 mg/L. There is information from the owner of the tested sample (Chevron Oronite) that the sample tested was CAS 122384-87-6 and a commercial sample containing 50% A.I. and 50% oil. Therefore the end results for the Registration material are considered to be an LC/LL50 value greater than or equal to 500mg/l. 
Saltwater results: In an acute toxicity study conducted in accordance with GLP to the methodologies set out in OECD guideline 203, the 96-hour mortality of the fish tested at 100% Water Soluble Fraction (WSF) was 5% . The LL50 can be considered to be >10 g/l. There is no specific sample information about oil % for this sample but generic commercial samples for this CAS are up to 40% oil, and this is assumed to apply here. Therefore the LL50 for the Registration material (oil-free) is assumed to be 60% of the tested result, i.e. 6000mg/l. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

500 mg/L

Marine water fish

Marine water fish

Effect concentration:

6 000 mg/L

Additional information

There are no available data for this endpoint for CAS: 96152-43-1, therefore studies from supporting, structurally similar substances have been used for read-across purposes to address this endpoint. Where samples have been tested in oil, the end results have been adjusted to account for the fact that the Registration material does not contain oil.

The following studies are used to address this endpoint:

- In Ward, 1997, the acute toxicity of the water accommodated fraction (WAF) of a 1,000 mg/L mixture of the test material and water to the rainbow trout, Oncorhynchus mykiss, was investigated during a study conducted at T.R. Wilbury Laboratories, Inc. The test, which was designed to determine the toxicity of the WAF of the test substance, was performed from December 9 to 13, 1996 and was conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test) and GLP.

The reliability rating for this study is 1, however this is being used as read across to a supporting substance as there was no available data to fulfil this endpoint for the test material and so the reliability rating will be reduced to 2, according to the criteria of Klimisch, 1997. This study is being used as read across from Phenol, tetrapropenyl-, sulfurized, calcium salts CAS No. 68855-45-8.

- In Ward and Boeri, 1993, the acute toxicity of the water accommodated fraction (WAF) of 100, 300, and 1,000 mg/L mixtures of the test material and water to the fathead minnow, Pimephales promelas, was investigated during a study conducted at the Envirosystems Division of Resource Analysts, Inc. and T.R. Wilbury Laboratories, Inc.

The test, which was designed to determine the toxicity of the WAFs of test substance, was performed from August 6 to 10, 1991

and was conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test) and GLP.

The reliability rating for this study is 1, however this is being used as read across to a supporting substance as there was no available data to fulfil this endpoint for the test material and so the reliability rating will be reduced to 2, according to the criteria of Klimisch, 1997. This study is being used as read across from Phenol, tetrapropenyl-, sulfurized, carbonates, calcium salts, overbased CAS No. 122384-87-6/68784-26-9. This is the key study for the endpoint based on the variety of test concentrations and characterisation information thus provided.

- The supporting T.J. Ward, 1993 study (T.R. Wilbury Laboratories, Inc., report number: 9176-CMA/ESI) has a reliability rating for this study of 1, however this is being used as read across to a supporting substance as there was no available data to fulfil this endpoint for the test material and so the reliability rating will be reduced to 2, according to the criteria of Klimisch, 1997. This study is being used as read across from Phenol, tetrapropenyl-, sulfurized, calcium salts CAS No. 68855-45-8. Nominal concentrations: 96-h LL50 (reported as “LC50” in the report) >1,000 mg/L. This is equivalent to 96-h LL0 (reported as “NOEC” in the report) of 1,000 mg/L. Test results reported in the study as “lethal concentrations” are reported in this summary as “lethal loading rate”, because test results are based on WAF loading rates.

Marine conditions:

- The Nicholson R.B. and Breteler R.J. 1986 study (Springborn Bionomics, Inc. report number: BW-86-04-1981) was not considered a key study for this endpoint as it was conducted less recently and also it was conducted using seawater and a fish native to brackish waters as opposed to the more typical freshwater conditions used in testing for this endpoint. However for chemical safety assessment purposes, the LC50 from this study will satisfactory fulfil the marine key study endpoint.

The reliability rating for this study is 1, however this is being used as read across from a supporting substance as there was no available data to fulfil this endpoint for the test material and so the reliability rating will be reduced to 2, according to the criteria of Klimisch, 1997. This study is being used as read across from Phenol, tetrapropenyl-, sulfurized, calcium salts CAS No. 68855-45-8.

The 96-hour mortality of the fish tested at 100% Water Soluble Fraction (WSF) was 5%. The LC50 can therefore be considered to be >10 g/l.