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EC number: 207-630-7 | CAS number: 486-25-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 Dec 2018 - 28 Dec 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- The duration of the test was 14 days instead of 28 days, as stated in the OECD guideline. The validity criteria were met, thus the test is considered valid.
- Qualifier:
- according to guideline
- Guideline:
- ISO 9888 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Fluoren-9-one
- EC Number:
- 207-630-7
- EC Name:
- Fluoren-9-one
- Cas Number:
- 486-25-9
- Molecular formula:
- C13H8O
- IUPAC Name:
- 9H-fluoren-9-one
- Test material form:
- not specified
- Details on test material:
- Tested samples were water samples containing several contaminants. No further details on the test substance were reported for this sample.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum: taken from the Sponsor's wastewater treatment plant (no further details provided)
- Concentration of sludge: 2.0 g suspended substance/L
- Initial cell/biomass concentration (of suspended substance): 29 g suspended solids/L - Duration of test (contact time):
- 14 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 0.06 mg/L
- Based on:
- test mat.
- Remarks:
- spike concentraion of 9-fluorenone
- Initial conc.:
- ca. 514 - ca. 525 mg/L
- Based on:
- DOC
- Remarks:
- non-spiked samples [flasks F(TI) and F(TII)]
- Initial conc.:
- ca. 514 - ca. 532 mg/L
- Based on:
- DOC
- Remarks:
- spiked samples [flasks F(TIII) and F(TIV)]
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- other: Chemical Oxygen Demand (COD)
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20-25°C
- pH: adjusted to 7.0 +/- 0.5
- Suspended solids concentration: 29 g/L
- Continuous darkness: diffused light
TEST SYSTEM
- Culturing apparatus: flasks (2 L and 250 mL)
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aerobic conditions were maintained by bubbling air from an air pump through a long glass tube attached to the bottom of the flask
- Measuring equipment: not specified
- Other: before taking samples from the flasks, water loss due to evaporation was made up with deionized water. After sampling, a new mark was made at the new medium level.
SAMPLING
- Sampling frequency: on t=0h, 3h, 3d, 7d and 14d. Except for flasks containing sterile medium, which were only samples on t=0 and 14d.
- Sampling method: not specified
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, consisting of 138 mL inoculum, added up to 2000 mL with deionized water
- Abiotic sterile control: yes, consisting of the sample spiked with the test substance and deionized water. The sterile medium was filtered through a membrane filter and kept under sterile conditions to prevent bacterial growth.
- Toxicity control: yes, containing the sample spiked with the test substance, the reference compound (ethylene glycol) and inoculum.
STATISTICAL METHODS: no statistical analysis was performed.
CALCULATION OF RESULTS:
The percentage degradation of DOC (or COD) was calculated with the following equation:
DT=(1-(ct-cBt)/(c0-cB0))*100
where
c0 is the concentration of DOC (or COD) at time 0 in each test flask.
cB0 is the concentration of DOC (or COD) at time 0 in the blank flask.
ct is the concentration of DOC (or COD) at time t in each test flask.
cBt is the concentration of DOC (or COD) at time t in the blank flask.
Reference substance
- Reference substance:
- ethylene glycol
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 85
- Sampling time:
- 14 d
- Remarks on result:
- other: non-spiked sample Flask TI
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 86
- Sampling time:
- 14 d
- Remarks on result:
- other: non-spiked sample Flask TII
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 86
- Sampling time:
- 14 d
- Remarks on result:
- other: spiked sample Flask TIII
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 85
- Sampling time:
- 14 d
- Remarks on result:
- other: spiked sample Flask TIV
- Details on results:
- - Based on <20% reduction of DOC after 3 h, it was shown that no physicochemical adsorption of the test substance to the inoculum had taken place.
- Toxicity to microorganisms could not be assessed as the experiment was interrupted before degradation has reached a plateau. However, the reduction of DOC in test vessels containing the test substance, showed that the sample did not inhibit microorganisms.
- The test substance was shown to be not inhibitory to the inoculum as degradation in the inhibition control corresponded to the degradation of the reference compound.
- DOC reduction in the abiotic sterile controls was <20%, showing that no significant abiotic elimination had taken place.
BOD5 / COD results
BOD5 / CODopen allclose all
- Parameter:
- COD
- Value:
- ca. 86 - ca. 87 other: % reduction
- Remarks on result:
- other: spiked samples Flask TIII and Flask TIV
- Parameter:
- COD
- Value:
- ca. 85 - ca. 86 other: % reduction
- Remarks on result:
- other: non-spiked samples Flask TI and Flask TII
- Results with reference substance:
- After 14 days, DOC content in the test sample containing the reference substance was 83 mg/L, indicating a DOC removal of 82%.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Degradation of the reference compound was >70% on day 14.
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- In the OECD 302B test, water samples spiked with Fluoren-9-one were biodegraded for 86% and 87% in duplicate samples tested, after 14 days of inoculation. Non-spiked samples were biodegraded for 85% and 86%. Based on these results, the samples can be considered inherently biodegradable.
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