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EC number: 275-532-1 | CAS number: 71487-01-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- March 21st, 1990 to April 14th, 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment it is considered appropriate to read across from quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides to structurally analogous quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
Furthermore, in certain organic solvents it has been reported that the exchange constants between nitrite and chloride in quaternary ammonium salts (QAS) are approximately equal. [Zhurnal Analiticheskoi Khimii, 2010, Vol. 65, No. 6, pp. 579–584. (E.M. Rakhman’ko, M.S. Markovskaya, L.S. Stanishevskii, Yu.S. Zubenko, A.R. Tsyganov)]
In this study Quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides, CAS Number 68391-05-9, EC Number 269-924-1) was subjected to a short term toxicity to fish study according to the OECD 203 protocol. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Arquad 2C-75
- Physical state: Clear yellow liquid
- Analytical purity: 76.4 % activity
- Impurities (identity and concentrations):
- Composition of test material, percentage of components: Activity 76.4%, solids: 75.0%, amine hydrochloride: 0.3%, free amine : 1.1%, colour Gardner: 1-2
- Lot/batch No.: L00963, CoA (Nov 1988)
- Stability under test conditions: Stable
- Storage condition of test material: at room temperature in the dark
- Other:
- Water solubility : Forms stable dispersions
- CRL code : T 90-2-1.10 - Analytical monitoring:
- no
- Details on sampling:
- Concentrations: 0.18, 0.23,0.3, 0.4 and 0.51 mg/L
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test substance formed a stable, homogeneous dispersion in water. The stock solution used contained 1 g of the test substance/L of deionized water. The test concentrations were prepared by addition of the respective amounts of stock dispersion to the dilution water (DSW), a synthetic water having a pH of approx 8.2 and a hardness of 13 dH. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Source: Local aquarium retailer (batch 15-03-90)
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 2.9 cm
- Weight at study initiation (mean and range, SD): 0.3 g( wet weight)
- Feeding during test: No
ACCLIMATION
- Acclimation period: atleast 12 d
- Acclimation conditions (same as test or not): Yes
- Feeding frequency: Fed one to three times each day for 6 d a wk - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No post observation period.
- Hardness:
- 13 degrees of hardness
- Test temperature:
- 21.7 to 22.3 °C
- pH:
- 7.4 - 8.1
- Dissolved oxygen:
- Fresh solutions: 8.8 mgO2/L, decreasing to 6.0 mgO2/L at 96h.
- Salinity:
- DSW:
100 mg of NaHCO3/L
20 mg of KHCO3/L
200 mg of CaCl2.2H2O/L
180 mg of MgSO4.7H2O/L
The deionized water which was used to prepare the DSW contained less than 10 μg of copper/L and a conductivity of less than 5 μS/cm. The TOC content was less than 2 mg/L - Conductivity:
- The deionized water which was used to prepare the DSW contained less than 10 μg of copper/L and a conductivity of less than 5 μS/cm. The TOC content was less than 2 mg/L
- Nominal and measured concentrations:
- 0 (control), 0.18, 0.23, 0.3, 0.4 and 0.51 mg/L Nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 5 L glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 5 L glass aquaria were filled with 3 L of test solution covered with glass plate
- Aeration: During the test the test media were not aerated
- Biomass loading rate: 0.7 g/L
OTHER TEST CONDITIONS
- Adjustment of pH: Yes
- Photoperiod: 12 hours of ambient light per day, provided by fluorescent tubes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.29-0.35 mg/L CL
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.27-0.32 mg/L CL
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.24-0.29 mg/L CL
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.24-0.29 mg/L CL
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.23 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - The highest concentration causing no mortality after 96 h was 0.23 mg/L.
- The lowest concentration causing 100 % mortality already after 72 h was 0.3 mg/L
- At 0.4 and 0.51 mg/L 100 % mortality was observed after 22 h.
- No other effects, such as deviations in the behaviour or appearance of the fish, were observed, except for the fish at the concentrations of 0.3 mg/L after 22 h and at 0.4 and 0.51 mg/L after 6 and 4 h, respectively, which showed a reduced activity.
The results observed in the definitive study are in good agreement with the results observed in the two preliminary tests. - Results with reference substance (positive control):
- The LC50 values for the reference substance are: 215 mg/L at 23 h, 183 mg/L after 48 h, 183 mg/L after 72 h and 183 mg/L after 96 h. The results obtained are in accordance with previous test results generated in an EEC report.
- Reported statistics and error estimates:
- The LC50 was determined using a statistical program of Griffioen (RIZA) based on a model of Kooyman (1981)
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the 96 h LC50 and NOEC value of the test material (76.4 % dicocodimethylammonium chloride) were 0.26 mg/L ( 95 % CL.: 0.24 - 0.29 mg/L) and 0.23 mg/L respectively
- Executive summary:
- A study was conducted to evaluate the acute toxic effects of test material (76.4 % dicocodimethylammonium chloride) to Brachydanio rerio (zebra fish) under semi-static conditions in accordance with EEC method C.1 and OECD guideline 203.
No chemical analyses of the test concentrations were performed during the test and all concentrations mentioned are nominal concentrations of the test material. On the basis of preliminary tests, the following concentrations were used in the definitive test: 0.18, 0.23, 0.3, 0.4 and 0.51 mg/L. Seven fish were used per test concentration and control. The concentration causing 100% mortality was observed within 72 h at 0.3 mg/L. Sub lethal effects were observed in all groups which also showed mortality.
Under the test conditions, the 96 h LC50 and NOEC value of the test material (76.4 % dicocodimethylammonium chloride) were 0.26 mg/L ( 95 % CL.: 0.24 - 0.29 mg/L) and 0.23 mg/L respectively.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- February 27th 1998 to March 6 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study conducted according to OECD guideline 203 in compliance with GLP
- Justification for type of information:
- Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment it is considered appropriate to read across from quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides to structurally analogous quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites
Furthermore, in certain organic solvents it has been reported that the exchange constants between nitrite and chloride in quaternary ammonium salts (QAS) are approximately equal. [Zhurnal Analiticheskoi Khimii, 2010, Vol. 65, No. 6, pp. 579–584. (E.M. Rakhman’ko, M.S. Markovskaya, L.S. Stanishevskii, Yu.S. Zubenko, A.R. Tsyganov)]
In this study Quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides, CAS Number 68391-05-9, EC Number 269-924-1) was subjected to a short term toxicity to fish study according to the OECD 203 protocol and the PARCOM Protocols on methods for the testing of chemicals used in the off-shore industry, part B: protocol for a fish acute toxicity test. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- other guideline: PARCOM Protocols on methods for the testing of chemicals used in the off-shore industry, part B: protocol for a fish acute toxicity test
- Deviations:
- yes
- Remarks:
- Study conducterd in saltwater
- Principles of method if other than guideline:
- PARCOM Protocols on methods for the testing of chemicals used in the off-shore industry, part B: protocol for a fish acute toxicity test.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Lilaflot 1590 (project sample code T98003)
- Physical state: Yellowish liquid
- Composition of test material, percentage of components: Dicocodimethyl quaternary ammonium compound chloride 60 %, amines tallowalkyl ethoxylate 18%, propan-2-ol 13 % and water 9 %
- Batch No.: 98-01-22
- Stability under test conditions: assumed to be stable at test and storage conditions
- Storage condition of test material: At room temperature in the dark
- Other:
- Water solubility : Dispersable - Analytical monitoring:
- no
- Details on sampling:
- No sampling performed
- Vehicle:
- no
- Details on test solutions:
- The test material was dispersible in water. A stock solution containing approximately 1 g/L of test substance was prepared as follows: to an accurately measured amount of approximately 1 g of test substance 80 mL of deionized water was added. The test substance dispersed well at room temperature when stirring. Thereafter, deionized water was added up to a final volume of 1000 mL. A homogeneous, clear, foaming stock solution was obtained. This was used for the preparation of the test solutions at the start of the test. For the refreshening of the test solutions on Day 2, a fresh stock solution of approximately 1 g/L was prepared as described above.
The test solutions were prepared by addition of the required amounts of stock solution to the dilution water. The following concentrations were tested: 0.05, 0.11, 0.24, 0.53 and 1.17 mg/L. A control containing only dilution water was included in the test. - Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: Sheepshead minnow
- Source: Bio international, Roemond, The Netherlands
- Length at study initiation (length definition, mean, range and SD): 2.4 cm
- Weight at study initiation (mean and range, SD): 0.29 g (wet weight of 5 fish).
- Feeding during test: No, 24 h or longer before the test
ACCLIMATION
- Type and amount of food: Dried or deep-frozen fish food
- Feeding frequency: Once a day
- Health during acclimation (any mortality observed): - Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- No data
- Test temperature:
- 21.0 to 21.5 degrees C
- pH:
- 7.8- 8.1
- Dissolved oxygen:
- 6.6 - 7.5 mgO2/L
- Salinity:
- 31.2 to 33.4 g/L
- Nominal and measured concentrations:
- Nominal: 0.05, 0.11, 0.24, 0.53 and 1.17 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 5L aquariums
- Type (delete if not applicable): Closed
- Material, size, headspace, fill volume: 5 L in size and contained 3 L of test solution covered with glass plates
- Renewal rate of test solution (frequency/flow rate): once in 48 h
- No of fishes/dose: 7
OTHER TEST CONDITIONS
- Photoperiod: 16 h of ambient light/d provided by fluorescent tubes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline:
- Range finding study: No
- Test concentrations: 0.05, 0.11, 0.24, 0.53 and 1.17 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.787 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.530- 1.170 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.787 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.530- 1.170 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.787 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.530- 1.170 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.787 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.530- 1.170 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - Dose groups of 1.17 mg/L caused 100 % mortality within 19 h.
- The NOEC was 0.53 mg/L.
- 96h LC50 was 0.787 mg/L.
- Reduced activity was only observed at the concentrations at which mortality occurred. Other sublethal effects such as deviations in the behaviour or the appearance of the fish were not observed. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The LC50 was calculated with a computer program (Akzo program SKBT, version 1.0) according to SOP L2, using both the trimmed Spearman-Karber method and the binomial test method.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the 96 h NOEC and 19 h LC100 of the test material (containing 60 % dicocodimethyl quaternary ammonium compound chloride, 18 % amines tallow alkyl ethoxylate, 13 % isopropanol and 9 % water 9 %) was determined to be 0.53 mg/L and 1.17 mg/L respectively. The 96 h LC50 of the test material was determined to be 0.787 mg/L ( at 98% CL: 0.470 and 1.170 mg/L).
- Executive summary:
A study was conducted to evaluate the acute toxicity of test material (containing dicocodimethyl quaternary ammonium compound chloride 60 %, amines tallow alkyl ethoxylate 18%, isopropanol 13 % and water 9 %) with the salt water fish, Cyprinodon variegates (sheepshead minnow) under semi-static conditions in accordance with OECD guideline 203 and the Parcom protocol on methods of the testing of chemicals used in the offshore oil industry in compliance with GLP.
Test fish were exposed to the test material for 96 h in the concentrations of 0.05, 0.11, 0.24, 0.53 and 1.17 mg/L.
Reduced activity was only observed at the concentrations at which mortality occurred. Other sub lethal effects such as deviations in the behaviour and appearance of the fish were not observed.
Under the test conditions, the 96 h NOEC and 19 h LC100 of the test material (containing 60 % dicocodimethyl quaternary ammonium compound chloride, 18 % amines tallow alkyl ethoxylate, 13 % isopropanol and 9 % water 9 %) was determined to be 0.53 mg/L and 1.17 mg/L respectively. The 96 h LC50 of the test material was determined to be 0.787 mg/L(98% CL: 0.470 and 1.170 mg/L).
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- February 27th, 1998 to March 6th, 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment it is considered appropriate to read across from quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides to structurally analogous quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
Furthermore, in certain organic solvents it has been reported that the exchange constants between nitrite and chloride in quaternary ammonium salts (QAS) are approximately equal. [Zhurnal Analiticheskoi Khimii, 2010, Vol. 65, No. 6, pp. 579–584. (E.M. Rakhman’ko, M.S. Markovskaya, L.S. Stanishevskii, Yu.S. Zubenko, A.R. Tsyganov)]
In this study Quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides, CAS Number 68391-05-9, EC Number 269-924-1) was subjected to a short term toxicity to fish study according to the OECD 203 protocol and the PARCOM Protocols on methods for the testing of chemicals used in the off-shore industry, part B: protocol for a fish acute toxicity test. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- other guideline: PARCOM Protocols on methods for the testing of chemicals used in the off-shore industry, part B: protocol for a fish acute toxicity test
- Deviations:
- yes
- Remarks:
- Study conducted in saltwater
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Arquad 2C-75 (project sample code T98001)
- Physical state: Clear yellow liquid
- Composition of test material, percentage of components: Dicocodimethylammonium chloride (75%)
- Batch No.: 98-01-22
- Stability under test conditions: Assumed to be stable at test and storage conditions
- Storage condition of test material: At room temperature in the dark
- Other:
- Water solubility : Dispersable - Analytical monitoring:
- no
- Details on sampling:
- No sampling performed
- Vehicle:
- no
- Details on test solutions:
- The test material was dispersible in water. A stock solution containing approximately 1 g/L of test substance was prepared as follows: to an accurately measured amount of approximately 1 g of test substance 80 mL of deionized water was added. The test substance dispersed well at room temperature when stirring. Thereafter, deionized water was added up to a final volume of 1000 mL. A homogeneous, clear, foaming stock solution was obtained. This was used for the preparation of the test solutions at the start of the test. For the refreshening of the test solutions on Day 2, a fresh stock solution of approximately 1 g/L was prepared as described above.
The test solutions were prepared by addition of the required amounts of stock solution to the dilution water. The following concentrations were tested: 0.21, 0.47, 1.03, 2.27 and 5.00 mg/L. A control containing only dilution water was included in the test.
The test was performed as a semi-static test with the test media replaced after 48 h. - Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: Sheepshead minnow
- Source: Bio international, Roemond, The Netherlands
- Length at study initiation (length definition, mean, range and SD): 2.4 cm
- Weight at study initiation (mean and range, SD): 0.29 g (wet weight of 5 fish).
- Feeding during test: No, 24 h or longer before the test
ACCLIMATION
- Type and amount of food: Dried or deep-frozen fish food
- Feeding frequency: Once a day
- Health during acclimation (any mortality observed): - Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- No data
- Test temperature:
- 21.5 to 22.5 degrees C
- pH:
- 7.8- 8.0
- Dissolved oxygen:
- 6.5 - 7.5 mgO2/L
- Salinity:
- 31.2 to 33.4 g/L
- Nominal and measured concentrations:
- Nominal: 0.21, 0.47, 1.03, 2.27, 5.00 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 5L aquariums
- Type (delete if not applicable): Closed
- Material, size, headspace, fill volume: 5 L in size and contained 3 L of test solution covered with glass plates
- Renewal rate of test solution (frequency/flow rate): once in 48 h
- No of fishes/dose: 7
OTHER TEST CONDITIONS
- Photoperiod: 16 h of ambient light/d provided by fluorescent tubes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline:
- Range finding study: No
- Test concentrations: 0.21, 0.47, 1.03, 2.27, 5.00 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.696 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.470-1.030 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.696 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.470-1.030 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.696 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.470-1.030 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.696 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98 % CL: 0.470-1.030 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - Dose groups of 1.03, 2.27 and 5.00 mg/L caused 100 % mortality within 19 h.
- The NOEC was 0.47 mg/L.
- 96h LC50 was 0.696 mg/L.
- Reduced activity was only observed at the concentrations at which mortality occurred. Other sublethal effects, such as deviations in the behaviour or the appearance of the fish, were not observed. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The LC50 was calculated with a computer program (Akzo program SKBT, version 1.0) according to SOP L2, using both the trimmed Spearman-Karber method and the binomial test method.
- Sublethal observations / clinical signs:
Number of surviving animals
Conc (mg/L)
0h
1h
2h
19h
24h
26h
44h
48h
51h
68h
72h
74h
91h
96h
Control
7
7
7
7
7
7
7
7
7
7
7
7
7
7
0.21
7
7
7
7
7
7
7
7
7
7
7
7
7
7
0.47
7
7
7
7
7
7
7
7
7
7
7
7
7
7
1.03
7
7
7
0
-
-
-
-
-
-
-
-
-
-
2.27
7
7
7
0
-
-
-
-
-
-
-
-
-
-
5.00
7
5
0
-
-
-
-
-
-
-
-
-
-
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the 96 h NOEC and 19 h LC100 value of the test material (75 % dicocodimethylammonium chloride) was determined to be 0.47 mg/L and 1.03 mg/L respectively. The 96 h LC50 of the test material was determined to be 0.696 mg/L at 98% confidence limits of 0.470 and 1.030 mg/L.
- Executive summary:
A study was conducted to evaluate the acute toxicity of test material (75 % dicocodimethylammonium chloride) with the salt water fish, Cyprinodon variegatus (sheepshead minnow) under semi-static conditions in accordance with OECD guideline 203 and the Parcom protocol on methods of the testing of chemicals used in the offshore oil industry in compliance with GLP.
Test fish were exposed to the test material for 96 h in the concentrations of 0.21, 0.47, 1.03, 2.27 and 5.00 mg/L. Reduced activity was only observed at the concentrations at which mortality occurred. Other sub lethal effects such as deviations in the behaviour and appearance of the fish were not observed.
Under the test conditions, the 96 h NOEC and 19 h LC100 value of the test material (75 % dicocodimethylammonium chloride) was determined to be 0.47 mg/L and 1.03 mg/L respectively. The 96 h LC50 of the test material was determined to be 0.696 mg/L at 98% confidence limits of 0.470 and 1.030 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment it is considered appropriate to read across from quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides to structurally analogous quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
Furthermore, in certain organic solvents it has been reported that the exchange constants between nitrite and chloride in quaternary ammonium salts (QAS) are approximately equal. [Zhurnal Analiticheskoi Khimii, 2010, Vol. 65, No. 6, pp. 579–584. (E.M. Rakhman’ko, M.S. Markovskaya, L.S. Stanishevskii, Yu.S. Zubenko, A.R. Tsyganov)]
In this study Quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides, CAS Number 68391-05-9, EC Number 269-924-1) was subjected to a short term toxicity to fish study according to the OECD 203 protocol. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Dodigen 1490
- Substance type: Surfactant
- Physical state: Light yellowish liquid
- Impurities (identity and concentrations): Water and isopropanol
- Lot/batch No.: E 06142685
- Expiration date of the lot/batch: 02-09-1989
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature
- Others:
- Density: 0.89 g/cm3
- pH value at 10 g/L = 4 - 6
- miscible with water - Analytical monitoring:
- no
- Details on sampling:
- Sampling not performed
- Vehicle:
- no
- Details on test solutions:
- Stock solution: 20 mg/L
0, 0.05, 0.1, 0.25, 0.5 and 1 mg/L. Dilutions were prepared with tapwater. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Source: Zoo Stumpe Hildesheim
- Length at study initiation (length definition, mean, range and SD): 3.2 cm
- Weight at study initiation (mean and range, SD): 0.42 g - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No post exposure observation period.
- Hardness:
- 7.6-7.8 mg/L
- Test temperature:
- 23 +/- 2 degrees C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 7.6 - 8.9
- Nominal and measured concentrations:
- Nominal: 0, 0.05, 0.1, 0.25, 0.5 and 1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 16 L aquarium
- Material, size, headspace, fill volume: 16 L aquarium
- No. of organisms per vessel: 10 fish/tank
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.57 - 0.77 CL
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - The % mortality was determined at 24, 48 , 72 and 96 h. The data shows that the mortality rate was the same from 24 to 96 h.
- Death caused by the test substance must occur in the first 24 h.
-The LC50 at 24 h was 0.63 mg/L. At 48, 72 and 96 h the LC50 was 0.66 mg/L - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not reported
- Sublethal observations / clinical signs:
Table 1: Mortality rates
Concentration mg/l
% Mortality
24 h
48 h
72 h
96 h
1
100
100
100
100
0.50
10
20
20
20
0.25
0
0
0
0
0.10
10
10
10
10
0.05
0
0
0
0
control
0
0
0
0
Table 2: Estimated LC50 values.
Time (h)
LC50 (mg/L)
P 95%
24
0.63
0.25-1.00
48
0.66
0.57-0.77
72
0.66
0.57-0.77
96
0.66
0.57-0.77
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the 96 h LC50 of the test material (containing dicocodimethylammonium chloride, isopropanol and water) was 0.66 mg/L. The lowest concentration which caused 100 % mortality was 1.00 mg/L. The lowest concentration without mortalities was 0.25 mg/L.
- Executive summary:
A study was conducted to test the acute toxic effects of the test material (containing dicocodimethylammonium chloride, Isopropanol and water) to the fresh water fish, Brachydanio rerio (new name: Danio rerio) under static conditions in accordance with OECD guideline 203 in compliance with GLP.
Test fish were exposed to the test material for 96 h in the concentrations of 0, 0.05, 0.1, 0.25, 0.5 and 1 mg/L.
Under the test conditions, the 96 h LC50 of the test material (containing dicocodimethylammonium chloride, Isopropanol and water) was 0.66 mg/L. The lowest concentration which caused 100 % mortality was 1.00 mg/L. The lowest concentration without mortalities was 0.25 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion.
In the case of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites that anion will be "nitrite" (NO2−) which will associate with other cations in solution. It is therefore prudent to consider read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method: Four series of 96h bioassays were conducted using rainbow trout. Series 1 (12 tests) and Series II (22 tests) were conducted over the pH range 6.4 - 9.0. The two series were conducted on two different size ranges of fish and in two different years. Series III (six tests) was conducted at pH 7 using three different acids (H2SO4, H3PO4 and HNO3) for pH reduction. Series IV (four tests) was conducted over the pH range 7.5 - 8.6 at chloride concentrations above background.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Constituent
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM- Common name: rainbow trout
- Source: Fish Cultural Development Center, US Fish and Wildlife Service, Bozeman, Montana, USA
- Age at study initiation (mean and range, SD): no data
- Length at study initiation: (see tables)
- Weight at study initiation: (see tables)
ACCLIMATION- Acclimation period: fish were acclimated to the test pH by gradually adjusting the dilution water pH over a 24-hour period to the desired value and maintaining that value for 48 hours before addition of sodium nitrite. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- See below under "Details on test conditions".
- Test temperature:
- See below under "Details on test conditions".
- pH:
- See below under "Details on test conditions".
- Dissolved oxygen:
- See below under "Details on test conditions".
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 62 L plastic tanks or 350 L circular glassfibre tanks
- Aeration: no
- Renewal rate of test solution: Approximately 5 hours for 62 L tank and 1.3 hours for 350 L tank.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Ground spring water
CASE A-1 to 12:
- Temperature; 9.0 - 10.7 degree C
- Hardness (mg/L CaC03); 185 - 208
- Alkalinity (mg/L CaC03); (see Table 1)
- Dissolved O2 (mg/L); 7.6 - 9.3
- pH; (see Table 1)
- CASE B-1 to 22:
- Temperature; 8.9 - 14.7 degree C
- Hardness (mg/L CaC03); 178 - 209
- Alkalinity (mg/L CaC03); (see Table 2)
- Dissolved O2 (mg/L); 6.6 - 9.3 - pH; (see Table 2)
CASE C-1 to 6:
- Temperature; 9.4 - 14.0 degree C - Hardness (mg/L CaC03); 192 - 201
- Alkalinity (mg/L CaC03); (see Table 3)
- Dissolved O2 (mg/L); 6.6 - 6.8 - pH; (see Table 3)
CASE D-1 to 4:
- Temperature; 9.9 - 10.7 degree C
- Hardness (mg/L CaC03); 199 - 206
- Alkalinity (mg/L CaC03); (see Table 4)
- Dissolved O2 (mg/L); 7.2 - 9.6 - pH; (see Table 4)
OTHER TEST CONDITIONS
- Photoperiod: no data
- Light intensity:Room light - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.54 - < 26.3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Values were converted from NO2-N into Na-NO2
- Details on results:
- The lowest 96-h LC50 was 0.11 mg/L NO2-N (equivalent to 0.54 mg/L NaNO2).
The highest 96-h LC50 was 5.35 mg/L NO2-N (equivalent to 26.3 mg/L NaNO2), in the presence of 10 mg/L chloride. - Sublethal observations / clinical signs:
Table 1: Acute toxicity of nitrite at different pH values to rainbow trout (Series I)
Avg fish wt (g)
Mean size length (cm)
Fish per tank
Acid or base
Alkalinity mg/L CaCO3
pH
96h-LC50NO2-N
285
28.1
5
H3PO4
109
6.44
0.21
171
23.2
10
H3PO4
171
7.50
0.20
46.7
15.3
20
H3PO4
174
7.52
0.32
53.1
15.7
20
None
176
7.68
0.27
387
29.7
5
None
159
7.74
0.24
60.5
16.6
20
None
177
7.76
0.27
188
23.6
10
None
172
7.81
0.19
24.3
12.3
20
None
179
8.10
0.28
121
20.5
10
NaOH
176
8.15
0.41
74.8
17.4
10
NaOH
180
8.23
0.38
69.8
17.0
10
NaOH
182
8.24
0.39
341
28.4
5
NaOH
190
9.00
1.67
The range of other water chemistry variables (in mg/L) for all tanks at time of tests were: dissolved oxygen 7.6-9.3; NH3-N 0.00-0.11; Cl- 0.00-1.71; Ca2+ 54.9-65.7; hardness (as CaCO3) 185-208. Temperature range for all tanks was 9.0-10.7°C
Table 2: Acute toxicity of nitrite at different pH values to rainbow trout (Series II)
Avg fish wt (g)
Mean size length (cm)
Fish per tank
Acid or base
Alkalinity mg/L CaCO3
pH
96h-LC50 NO2-N
12.8
10.4
10
H2SO4
12
6.99
0.14
15.3
10.2
10
H2SO4
128
7.02
0.11
10.4
10.0
10
H2SO4
135
7.24
0.15
10.0
9.4
10
H2SO4
150
7.37
0.19
9.3
9.2
10
H2SO4
160
7.59
0.18
7.0
9.1
10
None
188
7.83
0.40
12.8
10.3
10
None
173
7.86
0.21
13.2
10.3
10
None
177
7.87
0.22
8.0
8.6
10
None
170
7.88
0.36
10.0
9.3
10
None
172
7.90
0.21
8.2
8.7
10
None
170
7.91
0.30
3.1
6.3
10
None
165
7.94
0.25
10.4
9.2
10
None
174
7.95
0.17
8.4
8.9
10
NaOH
178
8.27
0.54
12.8
10.2
10
NaOH
185
8.41
0.46
15.5
10.9
10
NaOH
188
8.49
0.45
15.3
10.0
10
NaOH
186
8.61
0.50
8.2
8.8
10
NaOH
181
8.70
0.71
9.3
9.4
10
NaOH
188
8.83
1.17
10.5
9.6
10
NaOH
191
8.83
0.61
7.0
8.7
10
NaOH
202
9.03
1.10
8.0
8.8
10
NaOH
190
9.04
1.12
The range of other water chemistry variables (in mg/L) for all tanks at time of tests were: dissolved oxygen 6.6-9.3; NH3-N 0.00-0.06; Cl- 0.00-0.47; Ca2+ 46.8-61.3; hardness (as CaCO3) 178-209. Temperature range for all tanks was 8.9-14.7°C
Table 3: Acute toxicity of nitrite at pH 7 to rainbow trout using different acids (Series III)
Avg fish wt (g)
Mean size length (cm)
Fish per tank
Acid or base
Alkalinity mg/L CaCO3
pH
96h-LC50 NO2-N
15.3
10.2
10
H2SO4
128
7.02
0.11
12.8
10.4
10
H2SO4
120
6.99
0.14
13.2
10.7
10
H3PO4
152
6.99
0.18
13.7
10.8
10
H3PO4
150
6.98
0.18
13.7
10.6
10
HNO3
137
7.01
0.29
18.2
11.8
10
HNO3
136
7.01
0.26
The range of other water chemistry variables (in mg/L) for all tanks at time of tests were: dissolved oxygen 6.6-6.8; NH3-N 0.00-0.06; Cl- 0.10-0.63; Ca2+ 48.9-52.9; hardness (as CaCO3) 192-201. Temperature range for all tanks was 9.4-14.0°C
Table 4: Acute toxicity of nitrite at 10 mg/L chloride over different pH values to rainbow trout (Series IV)
Avg fish wt (g)
Mean size length (cm)
Fish per tank
Acid or base
Alkalinity mg/L CaCO3
pH
96h-LC50 NO2-N
28.2
12.9
10
HCl
174
7.50
3.74
79.0
17.6
10
None
177
7.90
3.54
147
22.0
10
NaOH
188
8.47
4.35
244
26.1
10
NaOH
184
8.59
5.34
The range of other water chemistry variables (in mg/L) for all tanks at time of tests were: dissolved oxygen 7.2-9.6; NH3-N 0.00-0.06; hardness (as CaCO3) 199-206. Temperature range for all tanks was 9.9-10.7°C
- Validity criteria fulfilled:
- not specified
- Conclusions:
- A publication from 1981, "Nitrite to Rainbow Trout (Salmo gairdneri): Effects of pH, Nitrite Species, and Anion Species", (Russo, R.C., Thurston, R.V. and Emerson, K. : Can. J. Fish. Aquatic. Sci 38, 387-393, which is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006], UNEP publications, reports an LC50 range for sodium nitrite to be 0.54 - 26.3 mg/L.
- Executive summary:
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9 was investigated in a publication entitled "Nitrite to Rainbow Trout (Salmo gairdneri): Effects of pH, Nitrite Species, and Anion Species", (Russo, R.C., Thurston, R.V. and Emerson, K. : Can. J. Fish. Aquatic. Sci 38, 387-393, 1981. This study was also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications, No guideline was followed and the study was not conducted to GLP.
The study was assigned a reliability score of 2 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).
Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion, the counter anion in this case being "nitrite" (NO2−), it is considered prudent to read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
The study reported in this publication determined an LC50 (96 h) range for sodium nitrite to be 0.54 - 26.3 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion.
In the case of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites that anion will be "nitrite" (NO2−) which will associate with other cations in solution. It is therefore prudent to consider read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method: Common eel were exposed over 96 h in a semistatic system
- GLP compliance:
- no
- Specific details on test material used for the study:
- Constituent
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Anguilla anguilla
- Details on test organisms:
- TEST ORGANISM
- Common name: common eel- Weight at study initiation (mean and range, SD): 21.9 ±5.6 g
ACCLIMATION
- Acclimation period: 2 weeks - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21.2 degrees C
- pH:
- 8.36
- Dissolved oxygen:
- 8.7 mg/L
- Nominal and measured concentrations:
- Test concentrations (nominal): 0, 120, 192, 307, 492 and 786 g/m³ NO2-N
Test concentrations (measured): 0, 121, 193, 318, 488 and 823 g/m³ NO2-N - Details on test conditions:
- TEST SYSTEM
- Renewal rate of test solution (frequency): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: IJmuiden (The Netherlands) tap water
- Na: 69 g/m³- K: 12 g/m³
- Ca: 76 g/m³
- Mg: 15 g/m³
- HCO3: 134 g/m³
- Cl: 117 g/m³- SO4: 106 g/m³ - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 708.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Value was converted from NO2-N into NaNO2
- Validity criteria fulfilled:
- not specified
- Conclusions:
- A publication from 1996, "The Acute Toxicity and Sublethal Effects of Nitrite on Growth and Feed Utilization of European Eel, Anguilla anguilla (L.)." (Kamstra A, Span JA, van Weerd JH : Aquacult. Res. 27, 903-911, which is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006], UNEP publications, {http://www.inchem.org/documents/sids/sids/7632000.pdf}) reports an LC50 of 708.2 mg/L.
- Executive summary:
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9) was investigated in a publication entitled "The Acute Toxicity and Sublethal Effects of Nitrite on Growth and Feed Utilization of European Eel, Anguilla anguilla (L.).", : (Kamstra A, Span JA, van Weerd JH : Aquacult. Res. 27, 903-911, 1996). This study is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications. No guideline was followed and the study was not conducted to GLP.
The study was assigned a reliability score of 2 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).
Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion, the counter anion in this case being "nitrite" (NO2−), it is considered prudent to read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
The study reported in this publication determined an LC50 (96 h) for sodium nitrite to be 708.2 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion.
In the case of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites that anion will be "nitrite" (NO2−) which will associate with other cations in solution. It is therefore prudent to consider read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method: According to the frequent seasonal extremes of temperature and salinity of the estuarine and coastal water where flatfishes were captured, two experimental conditions were established: the 'winter condition' (0% salinity and 12°C) and the 'summer condition' (30% salinity and 25°C).
- GLP compliance:
- no
- Specific details on test material used for the study:
- Constituent
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- other: Paralichthys orbignyanus
- Details on test organisms:
- TEST ORGANISM
- Common name: flatfish
- Source: Wild fish captured at the Cassino beach or at the estuary of the Patos Lagoon in Southern Brazil.
- Age at study initiation: juveniles
- Weight at study initiation: 88 ±17 g
- Feeding during test: no food
ACCLIMATION
- Acclimation period: Fish were acclimated in 1000 L tanks to 30% salinity and 20°C for 15 days. Water was continuously aerated
- Acclimation conditions (same as test or not):
- Type and amount of food: fry of mullet Mugil platanus or silverside Odonthestes sp. and small crustaceans like the shrimps Penaeus paulensis and Artemesia longinaris (ad libitum)
- Feeding frequency: daily - Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- Winter conditions: 12 °C; Summer conditions: 25 °C
- Salinity:
- Winter conditions: 0 %; Summer conditions: 30 %
- Nominal and measured concentrations:
- Test concentration ('winter conditions''; nominal): 0, 15, 20, 25, 30, 40, 60, 90 and 120 mg NO2-N/L.
Test concentration ('summer conditions''; nominal): 0, 10, 20, 25, 30, 40, 50 and 60 mg NO2-N/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: - Material, size, headspace, fill volume: 30 L plastic tanks
- Aeration: continuous
- Renewal rate of test solution: 100 % renewal daily
- No. of organisms per vessel: 10
OTHER TEST CONDITIONS
- Photoperiod: 12 h light and 12 h darkness - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 118.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Winter conditions. Values were converted from NO2-N into Na-NO2
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 150.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Summer conditions. Values were converted from NO2-N into Na-NO2.
- Sublethal observations / clinical signs:
LC50 (96 hr) of NO2-N for juveniles of the flatfish Paralichthys orbignyanus in "winter" and "summer" conditions. Data are expressed in mg/L. Data in brackets represent the 95% confidence limits of the LC50.
Time
Experimental conditions
Winter
Summer
24 h
107.86
55.78
(89.3 – 130.4)
(51.8 – 60.0)
48 h
52.98
38.52
(44.5 – 63.1)
(34.9 – 42.5)
72 h
33.69
31.30
(27.8 – 40.8)
(28.0 – 34.9
96 h
24.01
30.57
(21.3 – 27.1)
(27.3 – 34.2)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- A publication from 1996, "Toxicity of Nitrogenous Compounds to Juveniles of Flatfish Paralichthys orbignyanus." (Bianchini A, Wasielesky W Jr, Miranda Filho KC : Bull. Environ. Contam.Toxicol. 56, 453-459, which is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications, {http://www.inchem.org/documents/sids/sids/7632000.pdf}) reports LC50 values of 118.3 mg/L (Winter conditions) and 150.7 mg/L (Summer conditions).
- Executive summary:
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9) was investigated in a publication entitled " Toxicity of Nitrogenous Compounds to Juveniles of Flatfish Paralichthys orbignyanus", : (Bianchini A, Wasielesky W Jr, Miranda Filho KC : Bull. Environ. Contam.Toxicol. 56, 453-459, 1996). This study is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications. No guideline was followed and the study was not conducted to GLP.
The study was assigned a reliability score of 2 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).
Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion, the counter anion in this case being "nitrite" (NO2−), it is considered prudent to read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
The study reported in this publication determined LC50 values (96 h) for sodium nitrite to be 118.3 mg/L (Winter conditions) and 150.7 mg/L (Summer conditions).
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion.
In the case of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites that anion will be "nitrite" (NO2−) which will associate with other cations in solution. It is therefore prudent to consider read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method: Three different freswater fish species (Ictalurus punctatus, Tilapia aurea, Micropterus salmoides) were exposed over 96 hours in a static test system.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Constituent
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- other: Ictalurus punctatus (Channel Catfish), Tilapia aurea (Tilapia), Micropterus salmoides (Largemouth Bass)
- Details on test organisms:
- TEST ORGANISM
Largemouth Bass:
- Source: National Fish Hatchery and Technology Center, San Marcos, Texas
- Age at study initiation: no data
- Weight at study initiation (mean and range, SD): 2.8 g Channel Catfish:- Source: Texas Parks and Wildlife Department, San Marcos Fish Hatchery
- Age at study initiation: no data
- Weight at study initiation (mean and range, SD): 3.0 ±0.1 gTilapia:- Source: Texas Parks and Wildlife Department, San Marcos Fish Hatchery
- Age at study initiation: no data
- Weight at study initiation (mean and range, SD): 3.0 ±0.1 g
ACCLIMATION
- Acclimation period: min. 4 weeks
- Acclimation conditions (same as test or not): All fish were acclimatized in 252 L fiberglass tanks supplied by well water at a flow rate of seven turnovers per hour.
- Type and amount of food: commercial fish food (40% protein) ad libitum
- Feeding frequency: every 48 hours. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 202.4 4 mg/L as CaCO3
- Test temperature:
- 23 °C
- pH:
- 7.7 (max. 8.2)
- Dissolved oxygen:
- 8.1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: - Material, size, headspace, fill volume: 30 L glass aquaria.
- Aeration: Constant aeration to maintain dissolved oxygen levels near saturation.
- No. of organisms per vessel: 10 fish per dose (largemouth bass and channel catfish), 5 fish per dose (Tilapia).
- No. of vessels per concentration (replicates): three or more
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ground spring water
- Alkalinity: 165.5 mg/L as CaCO3- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Light intensity: room light - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 35 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Test organism: Channel Catfish; Value was converted from NO2-N into NaNO2
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 79.8 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Test organism: Tilapia; Value was converted from NO2-N into NaNO2
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 691 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Test organism: Largemouth Bass; Value was converted from NO2-N into NaNO2
- Sublethal observations / clinical signs:
Stability of the test chemical solutions: Measured nitrite concentrations ranged from 99.7+/-1.1% of nominal to 102.0+/-2.3% of nominal throughout the study.
Method of calculation: Median lethal concentrations were estimated using the method of Thompson (1947)
Analytical methods: Nitrite concentration determined using the method described by EPA (1974).
Determination of methemoglobin and plasma nitrite levels: Methemoglobin and plasma nitrite concentrations were determined using five fish per aquarium exposed to nitrite concentrations ranging from 1.5 to 194.9 mg/L. After 24 hours of exposure, fish were weighed, the caudal peduncle was severed, and blood was collected into capillary tubes from the haemal arch. Methemoglobin levels were determined spectrophotometrically (Evelyn & Malloy, 1938) after blood samples were lysed and centrifuged to remove turbidity. Total hemoglobin was determined according to Hainline (1958). Plasma nitrite levels were determined by a modification of the azo dye method (EPA 1974).
Analysis of variance followed by a student-Newman-keuls multiple range test was employed to test for difference among species.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The toxicity of nitrite varies considerably among species tested under similar water quality conditions. These differences are related to the the extent to which nitrite is concentrated in the plasma and the associated blood methemoglobin levels. Therefore, channel catfish have a lower resistance to nitrite than tilapia or largemouth bass because nitrite is more concentrated in catfish plasma. among the three species, largemouth bass has the greatest resistance to environmental nitrite as well as the lowest methemoglobin levels due to lower plasma nitrite concentrations. The low plasma nitrite levels may be indicative of a more selective chloride uptake cell located in the gills, resulting in exclusion of nitrite.
- Executive summary:
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9) was investigated in a publication entitled "Toxicity of Nitrite to Channel Catfish (Ictalurus punctatus), Tilapia (Tilapia aurea), and Largemouth Bass (Micropterus salmoides)", : ( Palachek & Tomasso : Can. J. Fish. Aquat. Sci. 41, 1739-1744, 1984). This study is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications. No guideline was followed and the study was not conducted to GLP.
The study was assigned a reliability score of 2 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).
Since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations in the aquatic environment and the counter anion, the counter anion in this case being "nitrite" (NO2−), it is considered prudent to read across from sodium nitrite (CAS Number 7632-00-0, EC Number 231-555-9) in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
The study reported in this publication determined LC50 values (96 h) for sodium nitrite to be 35 mg/L (Channel Catfish; Value was converted from NO2 -N into NaNO2), 79.8 mg/L
(Tilapia; Value was converted from NO2 -N into NaNO2) and 691 mg/L (Largemouth Bass; Value was converted from NO2 -N into NaNO2).
Referenceopen allclose all
Description of key information
Read across data is presented in order to demonstrate short term aquatic toxicity to fish of quaternary ammonium compounds, di-C12 -18 -alkyldimethyl nitrites.
The read across data is taken from Quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides, CAS Number 68391-05-9, EC Number 269-924-1 and from sodium nitrite, CAS Number 7632-00-0, EC Number 231-555-9. These substances were selected since quaternary ammonium substances are to be expected to dissociate into their quaternary ammonium cations and their counter anions in the aquatic environment.
Furthermore, in certain organic solvents it has been reported that the exchange constants between nitrite and chloride in quaternary ammonium salts (QAS) are approximately equal. [Zhurnal Analiticheskoi Khimii, 2010, Vol. 65, No. 6, pp. 579–584. (E.M. Rakhman’ko, M.S. Markovskaya, L.S. Stanishevskii, Yu.S. Zubenko, A.R. Tsyganov)]
Therefore, it is considered appropriate to read across from quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides to the structurally analogous quaternary dialkyldimethylammonium nitrites. In addition to this the counter anion is "nitrite" (NO2−) which will associate with other cations in solution. It is therefore prudent to consider read across from sodium nitrite in order to realise a fuller impression of the environmental properties of Quaternary ammonium compounds, di-C12-18-alkyldimethyl, nitrites.
Furthermore, in certain organic solvents it has been reported that the exchange constants between nitrite and chloride in quaternary ammonium salts (QAS) are approximately equal. (Zhurnal Analiticheskoi Khimii, 2010, Vol. 65, No. 6, pp. 579–584. (E.M. Rakhman’ko, M.S. Markovskaya, L.S. Stanishevskii, Yu.S. Zubenko, A.R. Tsyganov)
Therefore, four study endpoints are reported for Quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides, CAS Number 68391-05-9, EC Number 269-924-1 and four study endpoints are reported for from sodium nitite, CAS Number 7632-00-0, EC Number 231-555-9.
Quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides, CAS Number 68391-05-9, EC Number 269-924-1
1.
A study was conducted to evaluate the acute toxicity of test material (containing dicocodimethyl quaternary ammonium compound chloride 60 %, amines tallow alkyl ethoxylate 18%, isopropanol 13 % and water 9 %) with the salt water fish, Cyprinodon variegates (sheepshead minnow) under semi-static conditions in accordance with OECD guideline 203 and the Parcom protocol on methods of the testing of chemicals used in the offshore oil industry in compliance with GLP.
Under the test conditions, the 96 h NOEC and 19 h LC100 of the test material (containing 60 % dicocodimethyl quaternary ammonium compound chloride, 18 % amines tallow alkyl ethoxylate, 13 % isopropanol and 9 % water 9 %) was determined to be 0.53 mg/L and 1.17 mg/L respectively. The 96 h LC50 of the test material was determined to be 0.787 mg/L(98% CL: 0.470 and 1.170 mg/L).
2.
A study was conducted to evaluate the acute toxic effects of test material (76.4 % dicocodimethylammonium chloride) to Brachydanio rerio (zebra fish) under semi-static conditions in accordance with EEC method C.1 and OECD guideline 203.
Under the test conditions, the 96 h LC50 and NOEC value of the test material (76.4 % dicocodimethylammonium chloride) were 0.26 mg/L ( 95 % CL.: 0.24 - 0.29 mg/L) and 0.23 mg/L respectively.
3.
A study was conducted to evaluate the acute toxicity of test material (75 % dicocodimethylammonium chloride) with the salt water fish, Cyprinodon variegatus (sheepshead minnow) under semi-static conditions in accordance with OECD guideline 203 and the Parcom protocol on methods of the testing of chemicals used in the offshore oil industry in compliance with GLP.
Under the test conditions, the 96 h NOEC and 19 h LC100 value of the test material (75 % dicocodimethylammonium chloride) was determined to be 0.47 mg/L and 1.03 mg/L respectively. The 96 h LC50 of the test material was determined to be 0.696 mg/L at 98% confidence limits of 0.470 and 1.030 mg/L.
4.
A study was conducted to test the acute toxic effects of the test material (containing dicocodimethylammonium chloride, Isopropanol and water) to the fresh water fish, Brachydanio rerio(new name: Danio rerio) under static conditions in accordance with OECD guideline 203 in compliance with GLP.
Under the test conditions, the 96 h LC50 of the test material (containing dicocodimethylammonium chloride, Isopropanol and water) was 0.66 mg/L. The lowest concentration which caused 100 % mortality was 1.00 mg/L. The lowest concentration without mortalities was 0.25 mg/L.
Therefore, the lowest NOEC and LC50 values available are :-
NOEC = 0.23 mg/L (freshwater) and LC50 = 0.26 mg/L (freshwater)
NOEC = 0.47 mg/L (seawater) and LC50 = 0.696 mg/L (seawater)
Sodium nitite, CAS Number 7632-00-0, EC Number 231-555-9
1.
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9 was investigated in a publication entitled "Nitrite to Rainbow Trout (Salmo gairdneri): Effects of pH, Nitrite Species, and Anion Species", (Russo, R.C., Thurston, R.V. and Emerson, K. : Can. J. Fish. Aquatic. Sci 38, 387-393, 1981. This study was also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary sorce. No guideline was followed and the study was not conducted to GLP.
The study reported in this publication determined an LC50 (96 h) range for sodium nitrite to be 0.54 - 26.3 mg/L.
2.
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9) was investigated in a publication entitled "The Acute Toxicity and Sublethal Effects of Nitrite on Growth and Feed Utilization of European Eel, Anguilla anguilla (L.).", : (Kamstra A, Span JA, van Weerd JH : Aquacult. Res. 27, 903-911, 1996). This study is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications. No guideline was followed and the study was not conducted to GLP.
The study reported in this publication determined an LC50 (96 h) for sodium nitrite to be 708.2 mg/L.
3.
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9) was investigated in a publication entitled " Toxicity of Nitrogenous Compounds to Juveniles of Flatfish Paralichthys orbignyanus", : (Bianchini A, Wasielesky W Jr, Miranda Filho KC : Bull. Environ. Contam.Toxicol. 56, 453-459, 1996). This study is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications. No guideline was followed and the study was not conducted to GLP.
The study reported in this publication determined LC50 values (96 h) for sodium nitrite to be 118.3 mg/L (Winter conditions) and 150.7 mg/L (Summer conditions).
4.
The short-term toxicity to fish of sodium nitite (CAS Number 7632-00-0, EC Number 231-555-9) was investigated in a publication entitled "Toxicity of Nitrite to Channel Catfish (Ictalurus punctatus), Tilapia (Tilapia aurea), and Largemouth Bass (Micropterus salmoides)", : ( Palachek & Tomasso : Can. J. Fish. Aquat. Sci. 41, 1739-1744, 1984). This study is also referenced in the OECD Screening Information DataSet (SIDS) for Sodium Nitrite [2006] as a secondary source, UNEP publications. No guideline was followed and the study was not conducted to GLP.
The study reported in this publication determined LC50 values (96 h) for sodium nitrite to be 35 mg/L(Channel Catfish; Value was converted from NO2 -N into NaNO2), 79.8 mg/L
(Tilapia; Value was converted from NO2 -N into NaNO2) and 691 mg/L (Largemouth Bass; Value was converted from NO2 -N into NaNO2).