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EC number: 230-939-3 | CAS number: 7378-99-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 – 11 Dec 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Test item name: AD 1
Batch number: 4500083770EAS
Test item code: M-00000740
Appearance / colour: liquid / clear
Chemical name: N,N-dimethyloctylamine
Purity (nominal): > 95 % w/w
CAS number: 7378-99-6
Molecular weight: 157.3 g/mol
Relative density (nominal):0.765 g/cm³
Signal word(s): danger
Issue date of certificate: 21 Jan 2015
Expiry date: 23 Sep 2016
*Storage conditions: ambient ( ≤ +30 °C), dark, dry - Analytical monitoring:
- yes
- Details on sampling:
- Analytical data are required by the OECD guidelines to determine the concentration and stability of the test item over the entire test period.Due to limitation of the analytical method (LOQ = 1.00 mg/L AD 1) the stock solutions 15.0 and 3.00 mg/L and control were taken and analysed at test start (t = 0 hours) and after 72 hours.
- Vehicle:
- no
- Details on test solutions:
- Two stock solutions (S1 and S2) were prepared by directly weighing 15 mg into 1000 and 5000 mL AAP-medium, respectively. The stock solutions were homogenised by shaking and afterwards the solutions were clear and transparent. Both stock solutions were used for preparation of the test item concentrations V1 – V5 by dilution. All stock and test solutions were prepared with AAP-medium containing algae cells with a density of 0.5 × 104 algae per mL.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test organism is Pseudokirchneriella subcapitata Hindák, strain SAG 61.81. The algae used in this study are purchased from the MBM Sciencebridge GmbH, Hans-Adolf-Krebs-Weg 1, D-37077 Göttingen, Germany.The algae were grown semi-continuously in sterile cultures under permanent illumination in the laboratory. Old medium was periodically replaced by fresh mineral solution in order to keep the algae in an exponential growth state. Stock cultures were ordered regularly from the culture collection.
- Test type:
- static
- Water media type:
- other: AAP-Medium
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.0 - 22.4
- pH:
- 7.27 - 7.99
- Nominal and measured concentrations:
- see table below
- Details on test conditions:
- Test procedure: Static
Duration: 3 days (72 hours)
Initial cell density: 0.5 × 104 cells/mL
Temperature: 22.0 – 22.4°C
pH of control: 7.32 – 7.85
Illumination: Continuously from the side, 70.9 -82.1 µEm-2s-1
Culture flasks: 100 mL Erlenmeyer flasks with aluminium caps
CO2 supplied: By continuously shaking - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (EC50 growth rate = 1.77 mg/L; EC50 yield = 0.749 mg/L; EC50 biomass = 0.843 mg/L))
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Remarks:
- Growth rate
- Effect conc.:
- 0.048 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Remarks:
- Growth rate
- Effect conc.:
- 0.095 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- Growth rate
- Effect conc.:
- 0.264 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Remarks:
- Growth rate
- Effect conc.:
- 0.03 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Remarks:
- Growth rate
- Effect conc.:
- 0.01 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Remarks:
- Yield
- Effect conc.:
- 0.015 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Remarks:
- Yield
- Effect conc.:
- 0.025 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- Yield
- Effect conc.:
- 0.067 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Remarks:
- Yield
- Effect conc.:
- 0.03 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Remarks:
- Yield
- Effect conc.:
- 0.01 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- Statistically significant inhibitory effects for the test item were observed at concentrations of 0.0305 mg/L and above after 72 hours for growth rate and for yield. The EC50-value for growth rate (ErC50) was 0.264 mg/L. The EC50-value for yield (EyC50) was 0.0668 mg/L. The overall LOEC was determined to be at 0.0305 mg/L and the overall NOEC was determined to be at 0.00954 mg/L.
- Results with reference substance (positive control):
- Potassium dichromate is tested as the toxic reference item in a separate study twice a year to confirm the sensitivity of the test organism against compounds with known effects under the test conditions. The ECx values calculated in this reference test were considered to be within an acceptable range therefore it can be considered that the algae cells are sensitive.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item EC50-value for growth rate (ErC50) was 0.264 mg/L (nominal). The test item EC50-value for yield (EyC50) was 0.0668 mg/L (nominal). The LOEC was determined to be at 0.0305 mg/L and the NOEC was determined to be at 0.00954 mg/L (nominal) for growth rate and yield.
- Executive summary:
The measured content of AD 1 in both stock solutions was 83 and 86 % of nominal at test start. At test end the measured content of AD 1 was 72 % and 75 %. Since the measured concentrations at test start were within the range of 80 to 120% of nominal the toxicological endpoints were evaluated using the nominal concentrations.
Statistically significant inhibitory effects for the test item were observed at concentrations of 0.0305 mg/L and above after 72 hours for growth rate and for yield. The EC50-value for growth rate (ErC50) was 0.264 mg/L. The EC50-value for yield (EyC50) was 0.0668 mg/L.The overall LOEC was determined to be at 0.0305 mg/L and the overall NOEC was determined to be at 0.00954 mg/L.
Reference
Average cell number for each sampling time and concentration
Conc. |
Average cell numbers/mL1) |
|||
[mg/L] |
0 h |
24 h |
48 h |
72 h |
Control |
0.51 |
1.83 |
7.97 |
32.60 |
0.00954 |
0.51 |
1.63 |
8.23 |
35.74 |
0.0305 |
0.51 |
1.37 |
5.73 |
20.02 |
0.0977 |
0.51 |
1.34 |
5.17 |
15.64 |
0.313 |
0.51 |
1.15 |
2.59 |
3.37 |
1.00 |
0.51 |
0.84 |
1.00 |
0.62 |
1)Algae numbers are divided by 104.
Percentage inhibition of growth rate
Conc. |
% Inhibition of growth rate |
||
[mg/L] |
0 – 24h |
0h – 48h |
0h – 72h |
Control |
0.0 |
0.0 |
0.0 |
0.00954 |
9.0 |
-1.3 |
-2.41) |
0.0305 |
22.9 |
12.0 |
11.6* |
0.0977 |
24.8 |
15.6 |
17.5* |
0.313 |
36.5 |
40.7 |
54.5* |
1.00 |
62.0 |
75.5 |
96.0* |
1)Values were set to zero for ECx calculation
*statistically significant different to the control
Percentage inhibition of yield
Conc. |
% Inhibition of yield |
||
[mg/L] |
0 – 24h |
0h – 48h |
0h – 72h |
Control |
0.0 |
0.0 |
0.0 |
0.00954 |
15.2 |
-3.5 |
-9.81) |
0.0305 |
34.8 |
30.0 |
39.2* |
0.0977 |
37.1 |
37.5 |
52.9* |
0.313 |
51.5 |
72.1 |
91.1* |
1.00 |
75.0 |
93.4 |
99.7* |
1)Values were set to zero for ECx calculation
*statistically significant different to the control
Endpoints for test item after 72 h
|
Endpoint |
AD 1 [mg/L] nominal |
Growth rate |
ErC101) |
0.0483 |
|
95% confidential limits |
0.0126 – 0.0924 |
ErC201) |
0.0951 |
|
|
95% confidential limits |
0.0366 – 0.157 |
|
ErC501) |
0.264 |
|
95% confidential limits |
0.160 – 0.402 |
Yield |
EyC102) |
0.0152 |
|
95% confidential limits |
0.000898 – 0.0353 |
EyC202) |
0.0252 |
|
|
95% confidential limits |
0.00318 – 0.0531 |
|
EyC502) |
0.0668 |
|
95% confidential limits |
0.0254 – 0.163 |
|
LOEC3) |
0.0305 |
|
NOEC3) |
0.00954 |
1)probit analysis following the Gompertz distribution,
2)probit analysis following the normal distribution
3)Welch Bonferroni-Holms corrected for growth rate and yield
Concentrations of AD 1 in the test medium
AD 1 |
Sampling |
AD 1 found |
|
nominal (mg/L) |
mg L |
% of nominal |
|
0 |
0 h |
n.d. |
- |
72 h aged |
n.d. |
- |
|
3.00 |
0 h |
2.58 |
86 |
72 h aged |
2.24 |
75 |
|
15.0 |
0 h |
12.5 |
83 |
72 h aged |
10.8 |
72 |
Limit of quantification (LOQ) =1.00 mg/L AD 1
n.d. not detectable
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 66.8 µg/L
- EC10 or NOEC for freshwater algae:
- 9.54 µg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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