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EC number: 248-387-7 | CAS number: 27287-73-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 May 2016 to 09 June 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- Due to technical reason, temperature values (3 occasions, maximum of 25.5°C) outside the expected range of 17-23°C were recorded. This deviation is not considered to impact the results or integrity of this study as confirmed by the site veterinarian.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2-amino-6-methyl-1,2,4-triazolo[1,5-a]pyrimidin-5(1H)-one
- EC Number:
- 248-387-7
- EC Name:
- 2-amino-6-methyl-1,2,4-triazolo[1,5-a]pyrimidin-5(1H)-one
- Cas Number:
- 27287-73-6
- Molecular formula:
- C6H7N5O
- IUPAC Name:
- 2-amino-6-methyl-1,2,4-triazolo[1,5-a]pyrimidin-5(1H)-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- -Storage conditions: room temperature in the dark
-Colour: beige
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CRL:(WI)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, Research Models and Services, Germany GmbH, Sandhofer Weg 7, D-97633 Sulzfeld, Germany.
- Females (if applicable) nulliparous and non-pregnant: Yes
- Age at study initiation: Young adults (age range not given)
- Weight at study initiation: Between 221g and 254g (at dosing)
- Fasting period before study: Not reported
- Housing: Animals were housed individually in Type II. polypropylene/polycarbonate cages. Animals were housed with deep wood sawdust bedding to allow digging and other normal rodent activities.
- Diet (e.g. ad libitum): Yes
- Water (e.g. ad libitum): Yes
- Acclimation period: At least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21.0 – 25.5 °C
- Humidity (%): 30 – 68%
- Air changes (per hr): 15 - 20
- Photoperiod (hrs dark / hrs light): 12 hours light daily, from 6.00 a.m. to 6.00 p.m.
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- other: The test item was administered as supplied in a single dose. Sufficient water was used to dampen the test material to ensure good contact with the skin.
- Details on dermal exposure:
- TEST SITE
- Area of exposure: back of animal
- % coverage: Approximately 10% area of the toal body surface
- Type of wrap if used: Semi-occlusive plastic wrap
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Yes, using water at body temperature
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg body weight - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg body weight
- No. of animals per sex per dose:
- Five male and five female
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: Yes
- Other examinations performed: clinical signs, skin irritation, body weight
- Frequency of observations and weighing: Clinical observations at 1 and 5 hours after treatment and then daily. Body weights recorded on day ) (just before treatment) and on days 7 and 14.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality occured during the study
- Clinical signs:
- No adverse clinical signs or any type of local effect was observed after treatment with the test item or during the 14 day observation period
- Body weight:
- There were no treatment related changes in body weight. The body weights were within the range commonly recorded for the strain and animal age.
- Gross pathology:
- No macroscopic changes were observed
- Other findings:
- - Other observations: No treatment related skin irritation was observed in any animal throughout the study.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- An acute dermal toxicity study was performed according to OECD Guideline 402. The median lethal dose (LD50) of the test substance after a single dermal administration was greater than 2000 mg/kg bw in male and female CRL:(WI) rats.
- Executive summary:
An acute dermal toxicity study was performed according to OECD Guideline 402. Five male and five female CRL:(WI) rats were treated with a single, semi occlusive dermal application of the test substance at the limit dose of 2000 mg/kg body weight (bw). Test item was administered as supplied without dilution. The application period was 24 hours, followed by a 14-day observation period. Clinical observations along with a check of viability and mortality were performed on all animals at 1 and 5 hours after dosing and daily for 14 days thereafter. Body weight was measured prior to dosing on Day 0 and on Days 7 and 14. All animals were euthanized and subjected to a gross macroscopic examination at the end of the 2 weeks observation period.
No mortality occurred during the study. No adverse clinical signs or any type of local effect was observed after treatment with the test item or during the 14 day observation period. There were no treatment related changes in body weight. The body weights were within the range commonly recorded for this strain and age. No macroscopic changes were seen.
The median lethal dose (LD50) of the test substance after a single dermal administration was greater than 2000 mg/kg bw in male and female CRL:(WI) rats.
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