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EC number: 200-317-6 | CAS number: 57-15-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- Mutagenicity of cosmetics ingredients licensend by the European Communities
- Author:
- E. Gocke, M.-T. King, K. Eckhart, D. Wild
- Year:
- 1 981
- Bibliographic source:
- Mutation Research, 90 (1981) 91-109
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Test were performed according to standard procedures.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Chlorbutanol
- EC Number:
- 200-317-6
- EC Name:
- Chlorbutanol
- Cas Number:
- 57-15-8
- Molecular formula:
- C4H7Cl3O
- IUPAC Name:
- chlorbutanol
- Test material form:
- solid: crystalline
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- other: S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix from Aroclor-treated rats
- Test concentrations with justification for top dose:
- top dose: 3600 µg/plate
- Details on test system and experimental conditions:
- Chlorobutanol was tested in two different media: ZLM and VB. The concentration of citrate was 3.5 higher in VB medium than ZLM medium. The concentrations of the other ions are up to 2-fold higher in VB medium.
- Statistics:
- Significance was calculated according to the Kastenbaum-Bowman tables.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Remarks:
- in ZLM and VB medium
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 100
- Remarks:
- in ZLM and VB medium
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Remarks:
- in VB medium
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Remarks:
- in ZLM medium
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Species / strain:
- S. typhimurium TA 1535
- Remarks:
- in ZLM medium
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Remarks:
- in ZLM and VB medium
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Remarks:
- in ZLM and VB medium
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
Applicant's summary and conclusion
- Conclusions:
- Very extensive and reviewed study. Materials and methods are not described in details. Nevertheless, the study is valid and sufficient for classification.
- Executive summary:
Chlorobutanol and other cosmetic ingredients were tested for mutagenic effects: Ames test ( S. typhimurium TA 1535, TA 1537, TA 1538 TA 98 and TA 100), Basic test on Drosophila and the micronucleus test on mouse bone marrow. In all tests Chlorobutanol showed no mutagenic effects. Only in S. typhimurium TA 1535 in ZLM medium without metabolic activation Chlorobutanol revealed mutagenic effects. Tests with Chlorobutanol in typhimurium TA 1535 in ZLM medium with metabolic activation (S9 mix) and in VB medium with and without metabolic activation revealed no mutagenic potential. Overall and taking all results into account Chlorobutanol is not mutagenic. The positive result in S. typhimurium TA 1535 under specific conditions can be considered as false positive due to the high sensitivity of the Ames test.
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