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EC number: 457-660-1 | CAS number: 104797-47-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study with GLP. The study is well performed, except for using a loading rate of 100 mg of the insoluble test substance, and not a concentration at the solubility limit. Thereby mainly the enriched impurities of the substance were tested. A Klimisch 2 is allocated.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Actual concentrations of the test substance in the test media were determined immediately before placing the fish in the test vessels and then after 4 hours.
- Vehicle:
- no
- Details on test solutions:
- According to the scheme in Section 4.8 'Water solubility' a substance is classified as 'insoluble' if the solubility in water is <0.1 mg/L. The water solubility of T15 -AE is 0.077 mg/L at 20 °C.
Test medium was used to prepare the test substance solution.
The test medium with 100 mg/L loading rate was prepared by weighing in 0.512 g test substance and filling up to 5 L dilution water. This suspension was treated for 30 minutes by ultrasonication at approximately 20 °C. A water accommodated fraction, obtained after filtration with a 0.21µm cellulose acetate filter, was then tested.
Dilution water: Local tap water was mixed 1:1 with deionized water to achieve the appropriate water hardness of 250 mg CaCO3/L, determined in the control vessel. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Species: Zebrafish (Brachydanio rerio)
Supplier: Amazonas Aquarium, A-2840 Grimmenstein, Austria
Receipt of animals: 9 February 2005.
Length (cm): Approximately 2.5 cm (mean of the control group at the end of study).
Weight (g): ca. 0.3
Animal maintenance during acclimatisation and during exposure:
Water: Dilution water for holding and exposure
Aeration: Holding: continuous aeration by compressed air (water and oil filtered) and additional aeration by water recirculation using an EHEIM 2211 filter pump. Exposure: no aeration.
Temperature: Holding: ca. 20 °C. Exposure: 20 °C.
Tanks: Holding: full glass vessel (47 cm x 29 cm x 31 cm}, filled with ca. 40 L, covered with a transparent plastic lid. Water was purified by recirculation (power filter pump EHEIM 2211) through filter wool. Exposure: full glass vessels (29 cm x 20 cm x 24 cm}, filled with 5 litres of control or test medium, covered with transparent plastic lids.
Light: Artificial light from 4 a.m. to 8 p.m.
Feeding: Feed: TetraMin; Tetra Werke, Dr.rer.nat. Ulrich Baensch GmbH, D-49304 Melle. Holding: daily. Exposure: no feeding (last feeding about 24 hours before the start of the exposure).
Acclimatisation period: Approximately 9 weeks. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- n.a.
- Hardness:
- 250 mg CaCO3/L.
Local tap water was mixed 1:1 with deionized water to achieve the appropriate water hardness of 250 mg CaCO3/L, determined in the control vessel. - Test temperature:
- 20 °C
- pH:
- 7.8 - 8.1 in the test group.
7.7 - 8.1 in the control group. - Dissolved oxygen:
- 80 to 97 % in the test group.
93 to 98 % in the control group. - Salinity:
- n.a.
- Nominal and measured concentrations:
- Nominal loading rate: 100 mg/L.
- Details on test conditions:
- 7 fish were used per group.
- Reference substance (positive control):
- not required
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Concentration refer to the loading rate.
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Concentration refer to the loading rate.
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Concentration refer to the loading rate.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Concentration refer to the loading rate.
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Concentration refer to the loading rate.
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Concentration refer to the loading rate.
- Details on results:
- Mortality:
Negative control group: All animals survived until the end of the test.
Test substance group: All animals survived until the end of the test.
Animal observation:
All animals of the test substance group and of the negative control group were inconspicuous in behaviour and appearance until the end of the test.
Actual T15-AE concentration: At the start of theexposure the test substance concentration was 0.079 mg/L. Four hours after the start the test substance concentration was below the detection limit of the used method (0.0004 mg/L). - Results with reference substance (positive control):
- n.a.
- Reported statistics and error estimates:
- n.a.
- Sublethal observations / clinical signs:
Except for the maintenance of the test substance concentration all quality criteria were fulfilled:
• Constant conditions were maintained (temperature, pH).
• No mortality occurred in the negative control group.
• The dissolved oxygen concentration was higher than 60 % of the air saturation value throughout the test.
- Validity criteria fulfilled:
- yes
- Remarks:
- except for the
- Conclusions:
- The LC50,96h, based on the loading rate, is higher than 100 mg/L.
- Executive summary:
The acute toxicity of T15-AE and its degradation products to Zebrafish (Brachydanio rerio) was determined in a 96 hours static test according to the EU-method C.1. The solubility of T15-AE in water is very low. Therefore an aqueous extract (loading rate 100 mg/L in dilution water) was prepared to achieve a dissolved test substance concentration as high as possible. After approximately 30 minutes of ultrasonic treatment and subsequent filtration (filter size 0.2 µm) the obtained water accommodated fraction was tested (limit test). 7 fish were used each for the test substance group and for one control group (dilution water only). By this procedure predominantly the enriched and possibly better water soluble impurities are tested.
Results:
No mortality and no adverse effects were noted in the test substance group and in the negative control group.
The actual T15 -AE concentration of the filtrate was 0.079 mg/L at the start of the test and declined rapidly within 4 hours to below the detection limit.
pH and temperature were stable throughout the test period. Dissolved oxygen concentration was always higher than 60 % of the air saturation value at the appropriate temperature.
The LC50,96h, based on the loading rate, is higher than 100 mg/L.
Reference
Description of key information
The LC50,96h, based on the loading rate, for Brachydanio rerio is higher than 100 mg/L. The LC50 is higher than the solubility of the substance in the medium of ca. 0.08 mg/L. The result is not directly suitable for a classification, but it demonstrates that the mixture, i.e. the pure substance and its impurities, as far as they are soluble at 100 mg mixture/L, is not aquatic toxic.
The impurity benzothiazole-2-thiol (typically 1.2 % in T15-AE) is classified in CLP as Aquatic Acute 1 H400 and Aquatic Chronic 1 H410. The LC50 of Benzothiazole-2-thiol = 0.73 mg/L, the water solubility is 118 mg/L at 25 °C and the substance is not readily biodegradable (results obtained from the ECHA website). Applying the additivity formula of section 4.1.3.5.2. in CLP, an EC50 of the mixture (=T15-AE) of 61 mg/L is calculated. This is in acceptable agreement with the experimentally obtained result.
An LC50 far above the solubility limit is confirmed by a QSAR-calculation leading to an LC50 of 42 or 189 mg/L, depending on the model used.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 61 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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