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EC number: 285-089-6 | CAS number: 85029-63-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-12-07 to 2017-03-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Series on Testing and Assessment, No. 23, "Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures"
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.
Additional samples of the control were taken at test start and test end without any sample treatment. - Vehicle:
- no
- Details on test solutions:
- Concentrations of nominal 100, 45.5, 20.7, 9.4 and 4.3 mg/L and a control were tested.
Each concentration was prepared by dissolving test substance in test water and filtration.
Corresponding to mean measured concentrations of 44.2, 24.1, 14.6, 9.07 and 5.06 mg test item/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna (Straus), clone 5
Age at Test Start: From 1.0 to 18.5 hours old
Sex: Female
Origin: The Daphnia introduced in the test were taken from ibacon's in house laboratory culture.
The daphnids were bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids was performed in Elendt M4 medium. The test organisms were not first brood progeny. The daphnids in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Water Hardness: 2.5 mmol/L (= 250 mg/L) as Calcium Carbonate
- Test temperature:
- 19 to 22 °C at test start;
19 °C at test end - pH:
- 7.9 to 8.1 at test start;
7.7 to 7.8 at test end; and thus the pH-value did not vary by more than 1.5 units - Dissolved oxygen:
- 8.3 to 8.9 mg/L at test start;
8.5 to 8.9 mg/L at test end - Nominal and measured concentrations:
- Concentrations of nominal 100, 45.5, 20.7, 9.4 and 4.3 mg/L and a control were tested.
Corresponding to mean measured concentrations of 44.2, 24.1, 14.6, 9.07 and 5.06 mg test item/L. - Details on test conditions:
- Test Environment: Controlled environment room
Measurement of pH, Dissolved
Oxygen and Water Temperature:
The water temperature, pH-values and dissolved oxygen concentrations were determined at test start and test end in each treatment group.
Water Temperature:19 to 22 °C at test start; 19 °C at test end
pH-Values: 7.9 to 8.1 at test start;
7.7 to 7.8 at test end; and thus the pH-value did not vary by more than 1.5 units
Dissolved Oxygen Concentration: 8.3 to 8.9 mg/L at test start;
8.5 to 8.9 mg/L at test end
Light Regime: 16 h light : 8 h dark
Light Intensity: The light intensity was 200 to 710 lux (measured once during the test). - Reference substance (positive control):
- yes
- Remarks:
- For the evaluation of the quality of the Daphnia clone and the consistency of the experimental conditions, the reference item potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 24.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 14.6 mg test item/L. At the concentration of 24.1 mg test item/L, one animal was immobile and 20 animals were immobile at the concentration of 44.2 mg test item/L.
- Results with reference substance (positive control):
- In the most recent test with the reference item potassium dichromate the EC50 after 24 hours was determined to be 0.959 mg test item/L, indicating that the sensitivity of the daphnids was consistent with the level proposed by the OECD 202 guideline (EC50-24 h between 0.6 and 2.1 mg potassium dichromate/L).
- Reported statistics and error estimates:
- For the highest test concentration the mean measured value was used for the statistical analyses. In the results all values above the water solubility were determined to be above the water solubility limit (26 mg/L).The 24-hour and 48-hour EC50, EC20 and EC10 and the 95 % confidence limits were calculated by probit analysis.
The NOEC and LOEC after 24 and 48 hours were determined directly from the raw data.
The software used to perform the statistical analysis was ToxRat Professional, Version 3.2.1, ToxRat Solutions GmbH. - Validity criteria fulfilled:
- yes
- Remarks:
- Control Immobilisation Rate:Was 0 % and furthermore no daphnid showed signs of disease or stress Dissolved Oxygen Concentration:Was >= 8.5 mg O2/L in the control and test vessels at the end of the test. The validity criteria were met.
- Conclusions:
- 48h-EC50 (Daphnia magna, immobility) > 26 mg/L (above water solubility); 48h-NOEC (Daphnia magna, immobility) = 24.1 mg test item/L; 48h-LOEC (Daphnia magna, immobility) = 26 mg test item/L (mean measured)
- Executive summary:
The purpose of this study was to determine the influence of the test item Hostastat FE 20 LIQ on the mobility of Daphnia magna.For this purpose, young daphnids (< 24 hours old) were exposed in a static test to various concentrations under defined conditions for 48 hours. The recorded effects were the mobility of the daphnids after 24 and 48 hours.The test method of application and the test system are recommended by the test guidelines and Daphnia magna is the recommended test species.The purpose of the analytical part of this study is to verify the concentrations of the test item in the test medium.
Guidelines:
Commission Regulation (EC) No 440/2008, Annex, Part C, C.2.: "Daphnia sp. Acute Immobilisation Test", Official Journal of the European Union (EN), dated May 30, 2008
OECD Guideline for Testing of Chemicals No. 202: "Daphnia sp., Acute Immobilisation Test" adopted April 13, 2004
OECD Series on Testing and Assessment, No. 23, "Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures", December 15, 2000
SANCO/3029/99 rev.4 11/07/00: Residues: Guidance for generating and reporting methods of analysis in support of pre-registration data requirements for Annex II (part A; Section 4) and Annex III (part A; Section 5) of directive 91/414
Material and Methods
Test Item: Hostastat FE 20 LIQ; batch no.: ESD0021912; purity: 100 % (w/w), according to certificate of analysis
Test Species: Daphnia magna, clone 5; 1.0 to 18.5 hours old
Source: The daphnids introduced in the test were taken from ibacon's in-house laboratory culture.
Test Design: This study encompassed 6 treatment groups (5 dose rates of the test item and a control) each containing 20 individuals. The mobility of the daphnids was determined in a static 48-hour test by visual observation after 24 and 48 hours.
Endpoints: Number of immobile organisms after 24 and 48 hours
Test Concentrations: Concentrations of nominal 100, 45.5, 20.7, 9.4 and 4.3 mg/L and a control were tested.
Each concentration was prepared by dissolving test substance in test water and filtration.
Corresponding to mean measured concentrations of 44.2, 24.1, 14.6, 9.07 and 5.06 mg test item/L. The highest concentration is above the water solubility (26 mg test item/L).
Test Conditions: Water temperature: 19 to 22 °C; pH value: 7.7 to 8.1; dissolved oxygen concentration: 8.3 to 8.9 mg/L; photoperiod: 16 h light - 8 h dark; light intensity: 200 to 710 lux.
Results
Biological test results: After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 14.6 mg test item/L. At the concentration of 24.1 mg test item/L, one animal was immobile and 20 animals were immobile at the concentration of 44.2 mg test item/L.
Five % immobilisation at the concentration of 24.1 mg test item/L concentration are not considered as an effect, According to the validity criteria of the guideline, up to 10 % mortality is accepted in the controls and therefore can be expected as coincidental.
Conclusion: The toxic effect of the test item Hostastat FE 20 LIQ to Daphnia magna was assessed in a static concentration-response test. The 48-hour NOEC was determined to be 24.1 mg test item/L. Taking into account the water solubility of the substance (26 mg/L), the immobilization rate of 100 % at the highest concentration is clearly above the water solubility and cannot be used for the toxicity assessment.The 48-hour LOEC was determined to be 26 mg test item/L (above water solubility) and the 48-hour EC50 value was calculated to be > 26 mg test item/L (above water solubility). The quantification of the test item Hostastat FE 20 LIQ in the test samples was performed by analysing the Total Organic Carbon content (TOC).
This study was classified as acceptable and satisfied the guideline requirements for an acute toxicity study with freshwater invertebrates.
Reference
Description of key information
48 h-EC50 (Daphnia magna, immobility) > 26 mg/L (above water solubility); 48h-NOEC (Daphnia magna, immobility) = 24.1 mg test item/L; 48h-LOEC (Daphnia magna, immobility) = 26 mg test item/L (mean measured, OECD 202, 2017)
Note: The key value was inserted in order to enable the application of the PNEC calculator. The inserted concentration is higher than the effect concentration used as basis for the PNEC derivation and therefore does not bias the PNEC calculation performed by the PNEC calculator.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 26 mg/L
Additional information
In a reliable key study, acute toxicity of the submission substance on the mobility of Daphnia magna was investigated (RL1). Young daphnids were exposed to five test concentrations (nominal 100, 45.5, 20.7, 9.4 and 4.3 mg/L) for 48 hours in a static test. The test concentrations were analytically verified by analysing the Total Organic Carbon content (TOC). A control was run in parallel.
After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 14.6 mg test item/L. One animal was immobile at test concentration of 24.1 mg test item/L (5% effect), and 20 animals were immobile at 44.2 mg test item/L test concentration. Effects occurred in a dose-response relationship. Reported effect concentrations refer to mean measured concentrations: 46h-EC50 (Daphnia magna, immobility) > 26 mg/L (above water solubility); 48h-NOEC (Daphnia magna, immobility) = 24.1 mg test item/L; 48h-LOEC (Daphnia magna, immobility) = 26 mg test item/L.
This study was classified acceptable and satisfied the guideline requirements for fish acute test studies. In conclusion, the test was considered reliable and adequate for the environmental hazard assessment for aquatic organisms.
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