Ammonium dodecylbenzenesulphonate

Endpoint summary

Description of key information

The counter ion (ammonium) is not conisdered a potential irritant at low concentrations under neutral conditions, so the assessment is based on other salts.

Two GLP, Guideline in vitro tests were performed in order to assess the skin corrosive/irritating potential of LAS IPA salt and these are considered directly comparable to the ammonium salt. The results show that the substance is not corrosive to the skin, but it does have irritating properties. This is further supported by two older in vivo tests with benzenesulfonic acid, dodecyl-, branched, compd. with 2-propanamine. One GLP in vitro eye irritation test suggests that the substance is irritating to the eye. However, this test is not sofar officially accepted for regulatory purposes, and hence, additional information was used from an older sin vivo study performed with benzenesulfonic acid, dodecyl-, branched, compound. with 2-propanamine. The study suggest that the substance is an eye irritant.

Key value for chemical safety assessment

Skin irritation / corrosion

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Eye irritation

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin irritation

The potential skin effects of benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with 2-propanamine (97%) were tested in two in vitro studies with the use of reconstructed human epidermis models (EPISKIN^TM). The principle of the assays is based on the measurement of cytotoxicity in the human epidermal cultures following topical exposure to the test material, with the MTT reduction assay. The in vitro test for skin corrosion gave a negative result, while the in vitro test for skin irritation suggests that the substance has skin irritating properties. Additional supporting information taken from two in vivo studies performed with benzenesulfonic acid, dodecyl-, branched, compd. with 2-propanamine suggest that LAS IPA is an irritant to the skin of the rabbit.

Eye irritation

An in vitro eye irritation test was performed with the use of the SkinEthic Human Corneal Epithelium model in order to examine the eye irritation potential of benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with 2-propanamine (97%). The results showed a relative mean viability of the test item treated tissues after a 10 min exposure period of 16.6 %, i.e. the test the test item shall be considered an eye irritant. Nonetheless, currently, the aforementioned test is not fully validated on a regulatory context. Therefore, the classification of the test item cannot be based solely on this test. In an additional in vivo test performed with benzenesulfonic acid, dodecyl-, branched, compd. with 2-propanamine, eye irritation was also observed. Taken together, LAS IPA shall be classified and labelled as irritating to the eye.

Justification for selection of skin irritation / corrosion endpoint:

The weight of evidence approach was applied to address the endpoint of skin irritation, since no study available can  per se address this requirement; information from four available studies were used.

Justification for selection of eye irritation endpoint:

The weight of evidence approach was applied to address the endpoint of skin irritation, since no study available can  per se address this requirement; information from two available studies were used.

Effects on skin irritation/corrosion: irritating

Effects on eye irritation: irritating