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EC number: 207-894-3 | CAS number: 499-83-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- At one sampling point during the exponential degradation phase samples were tak-en after 4 days instead after 2 – 3 days. Because the available date were plausible and sufficient this can be stated as uncritical for the outcome of the study.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pyridine-2,6-dicarboxylic acid
- EC Number:
- 207-894-3
- EC Name:
- Pyridine-2,6-dicarboxylic acid
- Cas Number:
- 499-83-2
- Molecular formula:
- C7H5NO4
- IUPAC Name:
- pyridine-2,6-dicarboxylic acid
- Test material form:
- solid: crystalline
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 21. Apr. 2017, batch no: 20170421.
6.3.2.2 Pre-Treatment
The sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated until use. The dry matter was determined as 4040 mg suspended solids/L. - Duration of test (contact time):
- ca. 14 d
Initial test substance concentration
- Initial conc.:
- ca. 39.9 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its DOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2.
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 89.2
- Sampling time:
- 14 d
- Remarks on result:
- other: St.dev. could not be determined
Any other information on results incl. tables
Degradation values in %
Day |
Positive Control 1 |
Positive Control 2 |
Positive Control Mean |
Test 1 |
Test 2 |
Test Mean |
Abiotic Control |
Toxicity Control |
2 |
-0.6 |
1.5 |
0.5 |
11.1 |
2.0 |
6.5 |
2.4 |
2.0 |
4 |
17.9 |
21.2 |
19.5 |
33.8 |
21.4 |
27.6 |
1.8 |
45.5 |
8 |
55.6 |
69.1 |
62.3 |
87.9 |
76.0 |
81.9 |
1.0 |
81.6 |
10 |
68.8 |
80.7 |
74.7 |
94.0 |
83.1 |
88.6 |
0.6 |
86.3 |
14 |
84.4 |
86.7 |
85.5 |
98.3 |
91.2 |
94.7 |
0.4 |
89.2 |
18 |
85.0 |
84.7 |
84.8 |
96.5 |
90.3 |
93.4 |
0.4 |
88.1 |
23 |
86.8 |
85.4 |
86.1 |
98.2 |
88.6 |
93.4 |
0.1 |
88.8 |
29 |
86.4 |
82.9 |
84.6 |
98.2 |
88.9 |
93.5 |
-0.6 |
89.0 |
Because the values of day 29 are the sum of the IC values in scrubber flasks A and B, an increase (IC values in flasks B of the test higher than in those of the control) or a decrease (IC values in flasks B of the test lower than in those of the control) of degradation can be observed.
As the measured IC values in the abiotic control are very low, measurement uncertainties lead to negative degradation values while in fact no degradation has taken place.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- All validity criteria were met.
Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation was not observed. Both replicates of the test item showed good correspondence.
If degradation in the toxicity flask is below 25% after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 89.2 % after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 39.9 mg/L”.
Ready degradability is defined in the guidelines as degradation surpassing 60% within 10 days after reaching a level of 10%. Therefore, the test item Dipicolinic acid (DPA) can be considered as “readily biodegradable”.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid. - Executive summary:
The test itemDipicolinic acid (DPA)was tested using a concentration of nominally 20 mg organic carbon/L (corresponding to 39.7 mg Dipicolinic acid (DPA)/L) in test medium following OECD 301B and EU-Method C.4-C.
Aniline was chosen as positive control.
Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days.
All validity criteria were met. Degradation of the positive control was 62 % after 8 days.
The following data were determined for the test itemDipicolinic acid (DPA):
10-day-window: day 3 – 13
degradation at the end of 10-day-window 92 %
degradation at the end of the test 94 %
pass level following guideline: 60% at the end of 10-day-window for pure substancesrespective 60 % at the end of the test for mixtures
Therefore, when applying the 10-day-window, Dipicolinic acid (DPA)is readily biodegradable following OECD 301B and EU C.4-C respectively.
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