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EC number: 248-813-1 | CAS number: 28065-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Nov 2017 - 04 Jan 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
Test material
- Reference substance name:
- Oleic acid, compound with (Z)-octadec-9-enylamine (1:1)
- EC Number:
- 248-813-1
- EC Name:
- Oleic acid, compound with (Z)-octadec-9-enylamine (1:1)
- Cas Number:
- 28065-97-6
- Molecular formula:
- C34H69NO2 C36H67NO2 C36H69NO2 C36H71NO2 C36H73NO2
- IUPAC Name:
- oleic acid, compound with (Z)-octadec-9-enylamine (1:1)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: activated sludge from sewage plant at Taunusstein-Bleidenstadt, Germany
- Storage conditions: sludge was aerated for 1d with CO2-free compressed air (use of a 'CO2 scrubbing apparatus' described in the OECD Test Guideline 301 B)
- Storage length: 1d
- Pretreatment: The activated sludge was washed twice with mineral nutrient solution. After resolution with mineral nutrient medium the sludge was aerated by means of compressed humidified air for about 4 h. Before use as inoculum for the CO2-Evolution-Test the sludge was homogenised in a "Waring Blender" at low speed for 2 min. The filtrate was used as inoculum. Within the study the test solutions were stirred by means of magnetic stirrers in order to distribute the test item (or control item) and oxygen in a way to dissolve them at the maximum solubility.
- Water filtered: yes
- Type and size of filter used, if any: activated sludge was filtered through a cotton filter previously carefully rinsed with deionised water. The filtrate was used as inoculum (1% of the final volume of the test solution) on the same day of preparation. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 47.144 other: mg/3.5 L
- Based on:
- test mat.
- Initial conc.:
- ca. 10 mg/L
- Based on:
- TOC
- Initial conc.:
- 2 852 other: mg CO2/g test item
- Based on:
- ThCO2
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the guideline
- Solubilising agent: dichloromethane solvent was evaporated passively under a hood
- Test temperature: 20.2 to 22.3 °C
- Aeration of dilution water: The aeration rate of the test system was controlled at 4 L/h being assured by precise flowmeters
TEST SYSTEM
- Culturing apparatus: 5-L amber carboys closed (fill volume: 3500 mL) with stoppers with tubing for gas inlet and outlet (gas exit line)
- Number of culture flasks/concentration: 2 (per test item) and 1 reactor (for toxicity control, blank and reference, respectively)
- Method used to create aerobic conditions: Before starting the test, the mineral nutrient solution with inoculum but without any test component were aerated for 1d with CO2-free compressed air, in order to purge the system of carbon dioxide. Hereafter the test was started by addition of the test item. The gas outlet (exit air line) was connected to at least three CO2 absorber bottles filled with 100 mL 0.025 N Ba(OH)2 (connection in series), and bubbling of CO2 free compressed air through the solution was continued. Periodically the CO2 absorber nearest the test vessel was removed for analysis, and a new CO2 absorber was connected farest from the carboy. Precipitation of BaCO3 in the second CO2 trap indicated that the absorber bottle nearest the test vessel had to be changed and analysed for CO2.
- Measuring equipment: Degradation was calculated as a percentage of the theoretical CO2 (%TCO2) that should have been produced from the organic matter of the test item by complete combustion. The CO2 being generated was calculated to the nearest 0.01 mg and biodegradation values were rounded up to the nearest full percent (Schott Instruments, Titronic universal Ser.-No. 00693538).
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: Resting Ba(OH)2 (0.025 N) in the CO2 trap removed from the trap system was titrated with HCl (0.05 N) using phenolphthalein as an indicator. Each millilitre HCl of difference between the blank control and the test series with the test item titrated corresponds to 1.1 mg of CO2 produced. For this purpose values of titration for the 'Blank' were subtracted from that of the test unit with the test item.
SAMPLING
- Sampling frequency: t2d, t5d, t9d, t13d, t19d, t23d, t28d, and t29d
- Sampling method: At t28 d 1 mL conc. HCl was given into the test solutions in order to stop the test and to make the dissolved CO2 or inorganic carbon volatile and to purge the system of CO2
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 reactors
- Toxicity control: yes, 1 reactor
- Reference control: yes, 1 reactors
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 79
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of two replicates
BOD5 / COD results
- Results with reference substance:
- The biodegradation of the reference compound reached the pass level of 75% ThCO2 by day 9.
Any other information on results incl. tables
Table 1: 1st Test Solution, Test Item
Time |
Concentration: 47.144 mg / 3.5 L ThCO2: 134.5 mg CO2/ 3.5 L |
|
mg CO2 produced in the Test solution, cumulative |
% TCO2 |
|
2 |
3.18 |
2 |
5 |
23.63 |
18 |
9 |
76.14 |
57 |
13 |
84.82 |
63 |
19 |
92.87 |
69 |
23 |
100.77 |
75 |
28 |
107.31 |
80 **** |
29 |
112.13 |
83 ***** |
*TOC: Total Organic Carbon
**ThCO2: Theoretical amount of CO2 that can be generated by the test item
***% TCO2: Percentage of total CO2 the test item has generated in relation to the ThCO2
****The test solution was stopped by the addition of 1 mL conc. HCl;
*****The difference between this titration and that before is due to the liberation of CO2 after acidification of the test solution.
Table 2: 2nd Test Solution, Test Item
Time |
Concentration: 47.144 mg / 3.5 L ThCO2: 134.5mg CO2/ 3.5 L |
|
mg CO2 produced in the Test solution, cumulative |
% TCO2 |
|
2 |
2.58 |
2 |
5 |
25.03 |
19 |
9 |
71.16 |
53 |
13 |
80.53 |
60 |
19 |
88.74 |
66 |
23 |
92.97 |
69 |
28 |
96.47 |
72 **** |
29 |
101.42 |
75 ***** |
*TOC: Total Organic Carbon
**ThCO2: Theoretical amount of CO2 that can be generated by the test item
***% TCO2: Percentage of total CO2 the test item has generated in relation to the ThCO2
****The test solution was stopped by the addition of 1 mL conc. HCl;
*****The difference between this titration and that before is due to the liberation of CO2 after acidification of the test solution.
Table 3: Results of Sodium Benzoate
Time |
Concentration: 126.6 mg / 3.5 L ThCO2: 269.0 mg CO2/ 3.5 L |
|
mg CO2 produced in the Test solution, cumulative |
% TCO2 |
|
2 |
73.04 |
27 |
5 |
141.66 |
53 |
9 |
201.77 |
75 |
13 |
215.00 |
80 |
19 |
221.80 |
82 |
23 |
227.51 |
85 |
28 |
231.41 |
86 **** |
29 |
237.07 |
88 ***** |
*TOC: Total Organic Carbon
**ThCO2: Theoretical amount of CO2 that can be generated by the test item
***% TCO2: Percentage of total CO2 the test item has generated in relation to the ThCO2
****The test solution was stopped by the addition of 1 mL conc. HCl;
*****The difference between this titration and that before is due to the liberation of CO2 after acidification of the test solution.
Table 4 Toxicity Control - Oleylamine Oleate and Sodium Benzoate
Time |
Concentration: 126.4 mg Sodium Benzoate +47.144 mg Test Item ThCO2: 403.1 mg CO2/ 3.5 L |
|
mg CO2 produced in the Test solution, cumulative |
% TCO2 |
|
2 |
67.53 |
17 |
5 |
150.74 |
37 |
9 |
267.15 |
66 |
13 |
283.95 |
70 |
19 |
305.74 |
76 |
23 |
323.30 |
80 |
28 |
333.95 |
83 **** |
29 |
347.89 |
86 ***** |
*TOC: Total Organic Carbon
**ThCO2: Theoretical amount of CO2 that can be generated by the test item
***% TCO2: Percentage of total CO2 the test item has generated in relation to the ThCO2
****The test solution was stopped by the addition of 1 mL conc. HCl;
*****The difference between this titration and that before is due to the liberation of CO2 after acidification of the test solution.
Table 5: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
<20% |
Yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
70% |
Yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
70% |
Yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
<40 mg/L |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Calculated from the organic carbon content of the test item and the measured CO2 generation, 83% of the theoretical CO2 (ThCO2) has been generated by the test item within 28d of test period in the first culture with 47.144 mg of test item. 75.4% of the theoretical CO2 (ThCO2) has been generated by the test item within 28d in the second culture with 47.144 mg of test item. The calculated mean degradation value of the test item was 79%. Even the “10-days-window” was met though it is not considered for the kind of test item tested. Thus, the test item should be regarded as “readily biodegradable". The control item sodium benzoate was degraded 88%, and the conditions of the "10-days-window" were met within 9 days (75% degradation). The total CO2-evolution of the Blank was 93.4 mg CO2. Thus the test is regarded to be valid. The results obtained with the toxicity control indicate that there was no toxicity of the test item towards microorganisms at the concentration used within the test as a degradation value of >30% (here: 86%) was achieved in total within that treatment.
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