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EC number: 911-501-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-09 - 2018-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- Adopted July 22 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2015-03-09
Test material
- Reference substance name:
- Dioctyl hydrogen phosphate
- EC Number:
- 221-485-7
- EC Name:
- Dioctyl hydrogen phosphate
- Cas Number:
- 3115-39-7
- Molecular formula:
- C16H35O4P
- IUPAC Name:
- Dioctyl hydrogen phosphate
- Reference substance name:
- Octyl dihydrogen phosphate
- EC Number:
- 223-638-3
- EC Name:
- Octyl dihydrogen phosphate
- Cas Number:
- 3991-73-9
- Molecular formula:
- C8H19O4P
- IUPAC Name:
- octyl dihydrogen phosphate
- Reference substance name:
- Trioctyl phosphate
- EC Number:
- 217-305-1
- EC Name:
- Trioctyl phosphate
- Cas Number:
- 1806-54-8
- Molecular formula:
- C24H51O4P
- IUPAC Name:
- trioctyl phosphate
- Reference substance name:
- Octanol
- EC Number:
- 249-405-6
- EC Name:
- Octanol
- Cas Number:
- 29063-28-3
- Molecular formula:
- C8H18O
- IUPAC Name:
- octan-1-ol
- Test material form:
- liquid
Constituent 1
Constituent 2
impurity 1
impurity 2
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
The activated sludge was obtained from the sewage treatment works in Flörsheim/Main (Germany) treating predominantly domestic sewage.
- Method of cultivation:
Washing medium for activated sludge: reconstituted water (OECD 1992)
Frequency of washing: three times; between washings the activated sludge was centrifuged for 2 minutes at 1000 g
Storage time before test start: 2 days
Storage temperature: 20 ± 2 °C
During storage 50 mL (nominal) of synthetic sewage feed were added per litre activated sludge and per day. The activated sludge was stirred and aerated during storage
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20.2–20.7 °C (n = 34)
- pH:
- pH-value of the activated sludge: 6.8
pH value in the test solutions (before addition of the inoculum): 7.0–7.6 (adjusted with NaOH) - Dissolved oxygen:
- Aeration of test vessels: 0.5–1 L of air per hour (ambient air, oil-free air-compressor)
- Nominal and measured concentrations:
- 0, 62.5, 125, 250, 500, 1000 mg/L of test item
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL glass beakers (sterilised in a drying oven at 150 °C for 3 hours)
- Material, size, headspace, fill volume: Volume of test mixture per test vessel: 500 mL; Volume of microbial inoculum per test mixture: 200 mL; Volume of synthetic sewage feed per test mixture: 16 mL
- Aeration: 0.5–1 L of air per hour (ambient air, oil-free air-compressor)
- No. of vessels per concentration: 3
- No. of vessels per control: 6
- Sludge concentration (weight of dry solids per volume): 1.56 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic sewage = 16 g peptone; 11 g meat extract; 3 g urea; 0.7 g NaCl; 0.4 g CaCl2.2H2O; 0.2 g MgSO4.7H2O; 2.8 g K2HPO4; filled up to 1 litre with deionised water.
OTHER TEST CONDITIONS
- Adjustment of pH: Adjusted with NaOH
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The inhibition of the bacterial respiration activity, the 3-hour EC10 EC20 and EC50 including the 95% confidence limits were determined.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
The non-GLP range-finding test including a nitrification inhibition control showed less than 50% of control respiration at 1000 mg test item/L. Inhibition of nitrification was 70% of the control respiration (corresponding to 9.7% of control total respiration).
Since even at 1000 mg/L (highest level to be tested according to the guideline) the overall inhibition of total respiration was less than 50%, a separate evaluation of nitrification was not considered relevant.
There was less than 10% inhibition of total respiration at 100 or 10 mg/L.
A relevant specific inhibition of nitrification by the test item was not observed in the rangefinder. The definitive test was therefore performed without ATU.
- Test concentrations: 0, 62.5, 125, 250, 500, 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 356 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The definitive test showed a concentration-response relationship with an inhibition of total respiration rate of up to 36.7% of control respiration at 1000 mg test item/L
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: the 3-hour EC50 was found to be 6.7 mg/L thus laying in the range of 2-25 mg/L recommended by the guidelines. The result confirms the suitability of the activated sludge used.
Any other information on results incl. tables
Decrease of total respiration rate (inhibition in % of control) as caused by the test item after 3 h.
Treatment [mg test item/L] |
mean respiration rate [mg O2/(l*h)] |
standard deviation |
number of replicates |
inhibition of respiration rate [% of control value] |
Control |
69.9 |
5.29 |
6 |
0.0 |
62.5 |
69.4 |
6.26 |
3 |
0.7 |
125 |
68.2 |
4.85 |
3 |
2.4 |
250 |
61.2 |
6.20 |
3 |
12.5 |
500 |
62.7 |
5.63 |
3 |
10.3 |
1000 |
44.3 |
2.46 |
3 |
36.7 |
Selected effective concentrations (ECx) of the test item, and their 95%- confidence limits (according to Fieller's theorem).
Parameter |
EC10 |
EC20 |
EC50 |
EC80 |
Value [mg/L] |
356 |
613 |
1397 |
n.d. |
lower 95%-cl |
1.76 |
80.8 |
968 |
n.d. |
upper 95%-cl |
566 |
862 |
43783 |
n.d. |
cl: confidence limit; n.d.: not determined.
Results of the non-GLP range-finding test
Code |
Test conc. [mg test item/L] |
Mean respiration rate[mg O2/(L*h)] |
sd |
Inhibition [% of control] |
C0 |
0.0 |
53.3 |
2.66 |
- |
C1 |
10.0 |
54.1 |
- |
-1.6 |
C2 |
100 |
54.1 |
- |
-1.6 |
C3 |
1000 |
28.5 |
- |
46.5 |
|
|
|||
C0ATU |
0.0 |
34.9 |
- |
- |
C1ATU |
10.0 |
35.9 |
- |
-3.0 |
C2ATU |
100 |
31.7 |
- |
9.2 |
C3ATU |
1000 |
23.3 |
- |
33.1 |
|
|
|||
C0nitrif |
0.0 |
18.4 |
- |
- |
C1nitrif |
10.0 |
18.2 |
- |
0.9 |
C2nitrif |
100 |
22.4 |
- |
-22.1 |
C3nitrif |
1000 |
5.17 |
- |
71.9 |
Legend: Blank controls (C0), blank controls + ATU (C0ATU), treatments (C1: 10 mg test item/L; C2: 100 mg test item/L; C3: 1000 mg test item/L) with and without ATU. Nitrification-related respiration (C0nitrif, C1nitrif, C2nitrif, C3nitrif) calculated as difference between total and and ATU-inhibited respiration
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The CV% of O2 consumption rate in controls was ≤30%: 7.6%; The O2 consumption rate in the controls was ≥20: 44.9 mg O2/(g dw × h); The 3h-EC50 of 3,5-dichlorophenol was in the accepted range of 2–25 mg/L: 6.7 mg/L
- Conclusions:
- The 3 -hour EC10, EC20 and EC50 for Reaction mass of dioctyl hydrogen phosphate and octyl dihydrogen phosphate are 356, 613 and > 1000 mg/L, respectively.
- Executive summary:
The influence of the test item Reaction mass of dioctyl hydrogen phosphate and octyl dihydrogen phosphate on the activated sludge was evaluated by measuring the respiration rate under defined conditions, according to the OECD 209 Guideline. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item (0, 62.5, 125, 250, 500 and 1000 mg/L) after an incubation period of 3 hours.
The inhibition of the bacterial respiration activity was in the range of 0.7 to 36.7 % for the test concentrations of 62.5 to 1000 mg/L. The maximum inhibition was 36.7% at a test concentration of 1000 mg/L.
Based on measured inhibition rates, the 3 -hour EC10, EC20 and EC50 are 356, 613 and > 1000 mg/L, respectively. The definitive test showed a concentration-response relationship with an inhibition of total respiration rate of up to 36.7% of control respiration at 1000 mg test item/L.
The positive control 3,5 -Dichlorophenol was tested in the same way as the test item with nominal concentrations of 1.8, 5.9, 18.8 and 60.0 mg/L. The 3 -hour EC50 was found to be 6.7 mg/L thus laying in the range of 2 -25 mg/L recommended by the test guidelines. The result confirms the suitability of the activated sludge used.
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