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EC number: 231-601-8 | CAS number: 7647-18-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was generated according to valid testing guideline: OECD guideline 474
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- not specified
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Diantimony trioxide
- EC Number:
- 215-175-0
- EC Name:
- Diantimony trioxide
- Cas Number:
- 1309-64-4
- Molecular formula:
- Sb2O3
- IUPAC Name:
- dioxodistiboxane
- Test material form:
- not specified
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: male and female CD-1 mice supplied by Charles River Breeding Laboratories (Margate, UK)
- Age at study initiation: 5-11 weeks
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-23°C
- Humidity (%): 40-70%
- Photoperiod (hrs dark / hrs light): 12-hours light/dark cycle
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- 0.5% w/vhydroxypropylmethylcellulose in 0.1% w/v aqueous polysorbate 80.
- Duration of treatment / exposure:
- single oral gavage dose
- Frequency of treatment:
- once
- Post exposure period:
- Sampling times were 24 and 48 hours post dosing.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
400 mg/kg
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
667 mg/kg
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
1000 mg/kg
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5 male and 5 female
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Cyclophosphamide was used as positive control.
Examinations
- Tissues and cell types examined:
- polychromatic erythrocytes
- Details of tissue and slide preparation:
- DETAILS OF SLIDE PREPARATION:
- Slides were scored blindly.
METHOD OF ANALYSIS:
Two thousand polychromatic erythrocytes were examined for micronuclei per animal.
- Statistics:
- The statistical analysis consisted of a one-sided Student´s t-test on transformed data.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- A significant decrease in the percent polychromatic erythrocytes was only seen in females at the 24 h sampling time in the single dose study.
Any other information on results incl. tables
Table 1: Assessment of antimony trioxide in the mouse bone marrow micronucleus assay: single dose, male
treatment |
dose |
males |
|||
. |
. |
mean incidence of MPE/1000 PE ± SD |
mean % of polychromatic erythrocytes ± SD |
||
. |
. |
24 hours |
48 hours |
24 hours |
48 hours |
Vehicle control |
10 ml/kg |
1.5 ± 0.6 |
0.2 ± 0.3 |
42.5 ± 8.9 |
37.6 ± 11.1 |
Cyclophosphamide |
65 mg/kg |
19.2 ± 5.2** |
41.3 ± 5.2 |
||
Antimony trioxide |
5000 mg/kg |
0.8 ± 0.6 |
0.6 ± 0.7 |
41.9 ± 5.7 |
34.6 ± 13.9 |
PE: polychromatic erythrocytes; MPE: micronucleated polychromatic erythrocytes; SD: standard deviation. All means of MPE/1000 PE based on ten observations (two counts of 1000 PE per animal). All means of % PE based on five observations (one count of 1000 erythrocytes per animal). ** statistically significant increase or decrease over controls (p0.01 in Students t-test (one-sided) on transformed data). |
Table 2: Assessment of antimony trioxide in the mouse bone marrow micronucleus assay: single dose, female
treatment |
dose |
females |
|||
. |
. |
mean incidence of MPE/1000 PE ± SD |
mean % of polychromatic erythrocytes ± SD |
||
. |
.. |
24 hours |
48 hours |
24 hours |
48 hours |
Vehicle control |
10 ml/kg |
0.8 ± 0.5 |
0.6 ± 1.1 |
41.4 ± 8.8 |
44.2 ± 5.1 |
Cyclophosphamide |
65 mg/kg |
16.2 ± 2.8** |
43.0 ± 6.6 |
||
Antimony trioxide |
5000 mg/kg |
1.4 ± 0.9 |
1.2 ± 0.9 |
26.7 ± 7.2** |
39.9 ± 12.8 |
PE: polychromatic erythrocytes; MPE: micronucleated polychromatic erythrocytes; SD: standard deviation. All means of MPE/1000 PE based on ten observations (two counts of 1000 PE per animal). All means of % PE based on five observations (one count of 1000 erythrocytes per animal). ** statistically significant increase or decrease over controls (p0.01 in Students t-test (one-sided) on transformed data). |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
No statistically significant increase in the incidence of micronuclei was observed in the single or repeated dose study.
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