Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-370-7 | CAS number: 614-18-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethyl nicotinate
- EC Number:
- 210-370-7
- EC Name:
- Ethyl nicotinate
- Cas Number:
- 614-18-6
- Molecular formula:
- C8H9NO2
- IUPAC Name:
- ethyl nicotinate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling Intervals and Solution Composition: 0 Hour Exposure solutions in volumetric flasks prior to division into replicate vessels; 72 Hour Composite of all replicates within
each treatment and control; additional replicate for solution without algae.
Number of Samples Taken from Each Solution: One sample at each interval
Sampling Location: Approximate midpoint from the surface, bottom, and sides of the vessel
Sampling Device: Pipet
Archive Samples Collected: Yes
Quality Control (QC) Samples: Three samples per sampling interval, prepared in dilution
water at nominal concentrations approximating the test concentration range
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata, formerly Selenastrum
capricornutum
- Strain: 1648
- Source (laboratory, culture collection): UTEX The Culture Collection of Algae at the University of Texas, Austin, Texas (maintained in stock culture at Smithers Viscient)
- Age of inoculum (at test initiation):
- Method of cultivation: Three days since previous transfer
ACCLIMATION
- Acclimation period: N/A
- Culturing media and conditions (same as test or not): Same
- Any deformed or abnormal cells observed: N.D.
Study design
- Test type:
- static
- Water media type:
- other: Algal Assay Procedure (AAP) medium
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- N/A
- Test temperature:
- 23-25 celsius
- pH:
- 7.1-8.1
- Dissolved oxygen:
- N/A
- Salinity:
- N/A
- Conductivity:
- 92-96 µS/cm
- Nominal and measured concentrations:
- nominal: 0, 3.1, 6.3, 13, 25, 50, 100 mg/L
measured: 0, 2.4, 4.6, 10, 20, 37, 80 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250-mL glass flasks, fitted with stainless steel caps
which permitted gas exchange
- Material, size, headspace, fill volume: 100 mL
- Aeration: cultures stirred
- Initial cells density: 10,000 cells/mL
- Control end cells density:
- No. of organisms per vessel: 10^6
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): N/A
GROWTH MEDIUM
- Standard medium used: yes Algal Assay Procedure medium
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AAP (Algal Assay Procedure) medium
- Total organic carbon: 0.87 mg/L
- Particulate matter: N.D.
- Metals: None detected
- Pesticides: None detected
- Chlorine:None detected
- Culture medium different from test medium: no
- Intervals of water quality measurement: periodic (monthly)
OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH: yes- to 7.5 +-0.1
- Photoperiod: continuous
Light Intensity (photosynthetically-
active radiation, PAR):60 to 120 µE/m2/S maintained within ± 15%, i.e., 60 to 73 µE/m2/S, measured at initiation and at each 24-hour
interval during the exposure period using a Licor ModelLI-189 photometer and radiation sensor LI-190SA
Light Intensity (lux): Approximately 4870 to 5740 lux, measured at exposure initiation using a Fisher Scientific Traceable dual range
light meter
Bulb Type: Premira VitaLux fluorescent bulbs
Agitation: Continuous, 100 ± 10 rpm on an orbital shaker, rate monitored & recorded daily
- Light intensity and quality:
- Salinity (for marine algae): N/A
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter;
- Chlorophyll measurement: No
- Other:
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study
- Test concentrations:0, 0.01, 0.1, 1, 10, 100
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Zinc Chloride
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 74 - < 78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 79 - < 81 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 80 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 37 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 80 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 75 - < 77 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 76 - < 78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 77 - ca. 79 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- Reference Substance: Zinc chloride (ZnCl2)
Duration: 96 hours
Date Performed: 26 to 30 May 2017
EC50: 0.076 mg Zn/L, with 95% confidence intervals of
0.069 to 0.083 mg Zn/L (historical mean =0.089 mg/L, March 2005 to present)
A reference test was conducted at Smithers Viscient to evaluate the sensitivity of
Pseudokirchneriella subcapitata. The result was within the expected range for
Pseudokirchneriella subcapitata reported by Greene et. al. (1991); therefore, the culture is
considered of appropriate sensitivity for use in toxicity testing. - Reported statistics and error estimates:
- Response Variables Used for Statistical Analyses: 72-hour average specific growth rate and 72-hour yield
Endpoints Determined: No-Observed-Effect Concentration (NOEC), the highest test concentration which demonstrated no statistically adverse
effect (p ≤ 0.05) when compared to the control data ;
Lowest-Observed-Effect Concentration (LOEC), the lowest concentration which demonstrated a statistically adverse effectwhen compared to the control dat
EC10, EC20, EC50 (the concentrations of test substance which caused a 10, 20, or 50% reduction in the response variable compared to the control data)
Any other information on results incl. tables
Prior to NOEC and LOEC determinations, the data were first checked for normality using
Shapiro-Wilk’s Test (U.S. EPA, 2002) and for homogeneity of variance using Bartlett's Test
(U.S. EPA, 2002). If the data sets passed the tests for homogeneity and normality, then a
parametric statistical test, e.g., Dunnett’s Multiple Comparison Test (U.S. EPA, 2002) was used
to determine the NOEC and LOEC. If the data did not pass the tests for homogeneity and
normality, then the NOEC and LOEC were determined using an appropriate non-parametric
statistical test, e.g., Dunn’s Test with Bonferroni-Holm’s Adjustment (U.S. EPA, 2002). All
statistical determinations were made at the 95% level of certainty, except in the case of ShapiroWilk’s
and Bartlett's Tests, where the 99% level of certainty was applied.
If possible, EC10, EC20, and EC50 values were calculated using a nonlinear regression model.
The test concentrations bracketed the EC values so that the EC value came from interpolation
rather than an extrapolation. The EC10 , EC 20 , and EC 50 values were estimated so that (i) the
95% confidence intervals reported for the EC value did not contain zero and was not overly
wide, (ii) the 95% confidence interval for the predicted mean at the EC10, EC20, or EC50 value did
not contain the control mean and (iii) there was no significant lack-of-fit of regression model to
the data. If no concentration resulted in a 10, 20, or 50% reduction, the EC values were
empirically estimated to be greater than the highest concentration tested. CETIS Version 1.8
(Ives, 2013) was used to perform all statistical calculations.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72 hour specific growth rate EC50 for Pseudokirchinella subcapita exposed to the test substance is >80mg/L with no Confidence interval calculable. The 72 hour yield EC50 is 79 mg/L with a 95% confidence interval of 77-ND mg/L. The NOEC and LOEC are 37 mg/L and 80mg/L respectively for both growth rate and yield.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.