5-amino-3-[[4-[[4-[[4-anilino-2-hydroxyphenyl]azo]phenyl]amino]-3-sulphophenyl]azo]-4-hydroxynaphthalene-2,7-disulphonic acid, sodium salt

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 (48h): 64.9 mg/l (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

64.9 mg/L

Additional information

The following data was obtained for the Similar Substance 01. It is expected that the Target substance will present similar effect levels to daphnia. Justification for the use of a read-across approach is provided in Section 13 of IUCLID.

The acute toxicity of the substance to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline 202. The nominal concentrations tested were 31.3, 62.5, 125, 250 and 500 mg test substance/l. The 24-hour EC50 of the test substance was calculated to be 240.6 mg test substance/l with 95 % confidence limits from 204.5 to 283.0 mg test substance/l. After 48 hours test duration the toxicity of the test substance has highly increased. The 48-hour EC50 was calculated to be 64.9 mg test substance/l with 95 % confidence limits from 53.8 to 78.3 mg test substance/l. The calculated 48-hour EC10 was 35.5 mg test substance/l (95 % confidence limits: 26.4 47.8 mg/l), the 48-hour EC90 amounted to 118.8 mg test substance/l (95 % confidence limits: 89.0 - 158.5 mg/l). The 48-hour EC100 was determined to be 250 mg test substance/l.

In the control no immobility or mortality of the test animals was observed. The test substance concentrations in the analysed test media were in the range of 95.8 - 112.3 % of the nominal values during test duration. The test substance was sufficiently stable under test conditions.