Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 435-780-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- minor deviation to include use of DMF as a solvent
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- liquid: viscous
- Details on test material:
- - Appearance: brown viscous liquid
- Storage conditions: room temperature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the test additional sacrificial vessels with dispensed test solution were taken for whole sample analysis and at the end of the test, replicate vessels of the dilution water control, solvent control and the 1.0 mg/L test concentration was taken for whole sample analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- This study was run with a culture medium control, solvent control and nominal exposure concentration of 1.0 mg/L.
A primary stock concentrate of NITTA, with a nominal concentration of 10 g/L, was prepared by adding a nominal 0.1 g of test substance to 10 mL of dimethylformamide (DMF). The stock solution was used to prepare the test solution. This was achieved by the direct addition of the appropriate amount of concentrate to dilution water via a microlitre syringe into a stirring solution in a volumetric flask. The solvent control was prepared in the same way using solvent only. Test solutions were all observed to be clear and colourless. The appropriate test soluition (100 mL volume) was dispensed to each test and blank vessel.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata strain CCAP 278/4 from laboratory cultures maintained under axenic conditions. A 4-day old culture of the alga in the exponential growth phase was used as inoculum for the test. The culture was grown in the medium, and under the environmental conditions, described for the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- Range 20.5 - 22.3°C
- pH:
- Range 7.06 - 7.50
- Nominal and measured concentrations:
- 1.0 mg/L (nominal)
0.89 mg/L (mean measured concentration) - Details on test conditions:
- Six replicates of the culture medium control, solvent control and a single concentration of the test substance were employed. The position of each test replicate vessel in the incubator was randomised daily. One blank vessel (without algal inoculum) was incubated concurrently for each control and the single test concentration. The algal cell densities of the inoculum and test cultures were determined by electronic particle counting, using a Coulter counter and counting between a lower and upper threshold equivalent spherical diameter of approximately 2.3 and 5.0 μm respectively. After 24, 48, and 72 hours, samples were removed from each test and blank vessel. The appropriate blank particle count was subtracted from that of the test culture to obtain the cell density.
At the end of the test microscopic observations were made on samples taken from a single replicate of the control, solvent control and the 1.0 mg/L test substance concentration. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for NITTA is reported to be >1 mg/L (nominal cocentration) based on growth rate and NOEC is 1 mg/L.
- Executive summary:
Data available from a GLP-compliant study following OECD guideline 201, with minor deviation in that it employed the solvent DMF. Results are reported based on measured and nominal concentrations. This study is considered to be suitable for use as the key study for this endpoint. The EC50s for NITTA is reported to be >1.0 mg/L (nominal) or > 0.89 mg/L (measured; total dissolved and undissolved) based on growth rate and yield. These values are above the measure water solubilty of ca 0.3 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Παρόλο που ο Οργανισμός παρέχει μεγάλο μέρος του διαδικτυακού περιεχομένου στη γλώσσα σας, ένα μέρος αυτής της σελίδας είναι μόνο στα αγγλικά. Περαιτέρω πληροφορίες για την πολύγλωσση πρακτική του ECHA.
Καλώς ήρθατε στον δικτυακό τόπο του ECHA. Η παρούσα ιστοθέση δεν υποστηρίζεται πλήρως από τον Internet Explorer 7 (ή προγενέστερες εκδόσεις). Αναβαθμίστε τον φυλλομετρητή σας Internet Explorer σε πιο πρόσφατη έκδοση.
Ο παρών ιστότοπος χρησιμοποιεί cookies για να διασφαλίζεται ότι αποκομίζετε την καλύτερη δυνατή εμπειρία από την περιήγησή σας στους ιστοτόπους μας.
Μάθετε περισσότερα σχετικά με τον τρόπο που χρησιμοποιούμε τα cookies.