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EC number: 258-420-7 | CAS number: 53185-52-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 October 2006 - 19 December 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ''Method for Testing the Biodegradability of Chemical Substances by Microorganisms" stipulated in the "Testing Methods for new Chemical Substances" (November 21, 2003, Yakushokuhatsu No.1121002, November 13, 2003 Seikyoku No.2, Kanpokihatsu No.031121002)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Solubility in water: 300 g/100 g or more
Solubility in hexane: 50 g/100 g
Stability under storage conditions: stable - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: sludge was collected from 10 different sites in Japan, among which three city sewage plants, one industrial sewage plant, three rivers, one lake and two bays.
- Method of sampling: Sewage plants: return sludge from the sewage plants was collected; River, lake and sea: surface water and surface soil, which was in contact with the atmosphere, was collected.
- Preparation of sludge: in order to maintain uniformity of the inoculum used in the test, filtrate (5 L) of the supernatant of cultivated sludge (cultivated for approx. 3 months) was mixed with filtrate (5 L) of the supernatant of a sludge collected newly at each location. The mixture (10 L) was aerated after the pH value of the mixture was adjusted to 7.0 ± 1.0.
- Method of cultivation: Roughly 30 minutes after ceasing aeration of the sludge mixture, supernatant corresponding to about 1/3 of the whole volume was removed. Dechlorinated water was added to the remaining portion so that the total volume reached 10 L. This mixture was aerated (for 30 minutes or more). Subsequently, synthetic sewage was added to 50 g/L of the synthetic water so that the concentration of the synthetic sewage was 0.1% in the volume of dechlorinated water added. This procedure was repeated once every day. Cultivation was carried out at 25 ± 2 °C.
- Concentration of sludge: 2890 mg suspended solids/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 3 mL solutions were made up to 1L with purified water and the pH was adjusted to 7.0. This was according to OECD 301C.
- Test temperature: 25 +/- 1 °C
- pH: 6.1 - 7.0 in different vessels at the start of the test
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: 300 mL vessels
- Number of culture flasks/concentration:
Abiotic control (water + test item): 1
Positive control (aniline): 1
100 mg test item/L: 3
Blank control: 1
- Measuring equipment: closed system oxygen consumption measuring apparatus
- Details of trap for CO2: Soda lime was used as a CO2 absorber
SAMPLING
- Sampling frequency: at the end of the exposure period
- Sampling method: 10 mL of test solution was collected and centrifuged for 15 minutes, the supernatant was used for TOC analysis and HPLC analysis.
- Sample storage before analysis: samples were analysed directly after collection
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 1 replicate
- Abiotic sterile control: yes, 1 replicate
- Toxicity control: no
- Other: aniline was used as a reference control to test the activity of the sludge
STATISTICAL METHODS: none - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 2
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean of 3 replicates
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean of 3 replicates
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean of 3 replicates
- Details on results:
- - The test item was not sufficiently biodegraded by microorganisms to pass the criterium of 60% biodegradation.
- Results with reference substance:
- 78% biodegradation after 14 days and 87% biodegradation after 28 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- The reference item biodegraded for 78% in 14 days, the difference between vessels containing test solution was ≤6%, the BOD value of the blank control was 8.1 mg (corresponding to 27 mg O2/L).
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the conditions of the Modified Miti Test, MDMPA is not readily biodegradable.
- Executive summary:
A Biodegradation test was performed according to OECD 301C (Modified Miti Test) to assess the ready biodegradability of MDMPA over a period of 28 days. The concentration tested was 100 mg/L, with an activated sludge concentration of 30 mg/L (suspended solids). Besides three replicates containing test item, an abiotic control, a reference control and a blank control were included. At the end of the exposure period, samples from all test vessels were taken for TOC and HPLC analysis. Results showed the test item biodegraded for 1% based on oxygen consumption (BOD), 2% based on DOC and 0% based on HPLC analysis. Therefore, MDMPA is determined to be not readily biodegradable under the conditions of the Modified Miti Test. The reference item (aniline) biodegraded for 78% in 14 days. All acceptability criteria were met and the study was considered to be valid.
Reference
Description of key information
A Biodegradation test was performed according to OECD 301C (Modified Miti Test) to assess the ready biodegradability of MDMPA over a period of 28 days. The concentration tested was 100 mg/L, with an activated sludge concentration of 30 mg/L (suspended solids). Besides three replicates containing test item, an abiotic control, a reference control and a blank control were included. At the end of the exposure period, samples from all test vessels were taken for TOC and HPLC analysis. Results showed the test item biodegraded for 1% based on oxygen consumption (BOD), 2% based on DOC and 0% based on HPLC analysis. Therefore, MDMPA is determined to be not readily biodegradable under the conditions of the Modified Miti Test. The reference item (aniline) biodegraded for 78% in 14 days. All acceptability criteria were met and the study was considered to be valid.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Also an Inherent Biodegradation test was conducted according to OECD 302C and in compliance with GLP.
The inherent biodegradability of the test substance was determined in triplicate in a 28-day test (non-adapted micro-organisms) in an aerobic aqueous medium based on Biochemical Oxygen Demand (BOD) and the analysis of residual test substance in BOD bottles. In parallel, an abiotoc control, a reference substance and untreated control were tested.
During the test, the temperature was kept at 25±1 °C. The test was valid because the level of biodegradation of the reference substance sodium benzoate exceeded 40% after 7 days, and 65% after 14 days.
Based on the BOD results, biodegradation of the test substance was 3.83% during the testing period. Based on residue analysis, biodegradation of the test substance was 1.96% after 28 days. Therefore, the test substance is not inherently biodegradable under the conditions of the Modified MITI (II) test.
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