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EC number: 284-510-0 | CAS number: 84929-26-0 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Commiphora myrrha, Burseraceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-04-25 to 2018-XX-XX
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- none
- Analytical monitoring:
- yes
- Remarks:
- unspecific
- Details on sampling:
- - Sample storage conditions before analysis: Refrigeration (+2°C to +8°C); store in closed container.
- Vehicle:
- no
- Details on test solutions:
- The study was carried out using WAFs (Water Accommodated Fractions). The WAFs were prepared under closed conditions and by slow-stirring.
After 22 hours of gentle stirring in the dark at room temperature, the WAFs were allowed to stand for at least 1 hour before use. The first 100 mL were discarded via the drain port. Then the WAFs were filtered and added into test flasks containing a fixed amount of inoculum (5.103 cells.mL-1 per vessel) that were immediately sealed after filling with a minimum of headspace. At the start of the test, the solution was observed to be clear and colourless. - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Algae stock cultures were started by inoculating growth medium (= test water) with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 23 ± 2°C.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- n.a.
- Test temperature:
- 23 ± 2°C
- pH:
- 7.76 to 9.45
- Dissolved oxygen:
- >60% of saturation
- Salinity:
- n.a.
- Conductivity:
- n.a.
- Nominal and measured concentrations:
- nominal: 1, 3, 10, 31, 99 and 313 mg.L-1
- Details on test conditions:
- 100 mL, all-glass closed flasks with ground glass stopper, completely filled with test solution with minimum headspace.
Each test vessel was uniquely identified with study code, replicate number, date of experimentation and treatment group.
72hr
static - Reference substance (positive control):
- yes
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 60.371 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Details on results:
- 95% conf. limits = 27.425 – 178.292mg/L
- Results with reference substance (positive control):
- KA17-002, the 72h-EC50 was 1.457 mg.L-1 for the parameter growth rate.
- Reported statistics and error estimates:
- Probit analysis by Toxrat.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions and based on nominal loading rates of test item, the 72-hour EL50 for the parameters growth rate is 60.371mg/L.
- Executive summary:
A study was performed to assess the test item Essential oil of Commiphora myrrha for its ability to generate toxic effects on the unicellular algal species Pseudokirchneriella subcapitata. The followed method was designed to be compliant with OECD Guideline for Testing of Chemicals No. 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test", referenced as Method C.3 of Commission Regulation No. 440/2008 amended by Commission Regulation (EU) 2016/266 and with the “Guidance document on aquatic toxicity testing of difficult substances and mixtures” (OECD No. 23).
Following a preliminary range-finding test, algal cells were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading values of 1, 3, 10, 31, 99 and 313 mg.L-1 and to a control. The inhibition of growth in comparison to control cultures was determined over a test period of 72 hours, and thus over several algal generations. Concentration of dissolved organic material in the controls and the WAFs was checked by analysis of Total Organic Carbon (TOC) at the startand the end of the test.
The analytical results of this test showed that WAF concentrations were overall stable between the start and the end of the test, within or close to the ± 20% of the initial TOC concentrations values.
After 72 hours of exposure, ErLx and EyLx values were as follows:
Parameter
Growth rate (mg.L-1)
Yield (mg.L-1)
72-hour EL10
1.529
n.d.
95% conf. limits
0.055 – 5.310
n.d.
72-hour EL20
5.401
n.d.
95% conf. limits
0.603 – 13.626
n.d.
72-hour EL50
60.371
0.760
95% conf. limits
27.425 – 178.292
0.135 – 1.785
n.d.: not determined due to mathematical reasons or inappropriate data
Statistical analyses were performed by the computer program ToxRat
The toxic effect of the test item Essential oil ofCommiphora myrrhato the unicellular algal speciesPseudokirchneriella subcapitatawas investigated in a closed static test usingWater Accommodated Fractions. Under the experimental conditions andbased on nominal loading rates,the 72-hour EL50for the parameters growth rate and yield were determined to be 60.371 mg.L-1and 0.760 mg.L-1, respectively. The 72-hour EL10for growth rate was 1.529 mg L-1.
Reference
TheErLx and EyLx were as follows:
Parameter |
Growth rate (mg.L-1) |
Yield (mg.L-1) |
72-hour EL10 |
1.529 |
n.d. |
95% conf. limits |
0.055 – 5.310 |
n.d. |
|
|
|
72-hour EL20 |
5.401 |
n.d. |
95% conf. limits |
0.603 – 13.626 |
n.d. |
|
|
|
72-hour EL50 |
60.371 |
0.760 |
95% conf. limits |
27.425 – 178.292 |
0.135 – 1.785 |
n.d.: not determined due to mathematical reasons or inappropriate data
Description of key information
The toxic effect of the registered substance to the unicellular algal species Pseudokirchneriella subcapitata was investigated in a closed static test using Water Accommodated Fractions. Under the experimental conditions and based on nominal loading rates, the 72-hour EL50 for the parameters growth rate and yield were determined to be 60.371 mg.L-1 and 0.760 mg.L-1, respectively. The 72-hour EL10 for growth rate was 1.529 mg L-1.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 60.371 mg/L
- EC10 or NOEC for freshwater algae:
- 1.529 mg/L
Additional information
For that endpoint, an experimental GLP study with the registered substance was available.
This study was performed to assess the test item for its ability to generate toxic effects in the unicellular algal species Pseudokirchneriella subcapitata. The method followed was designed to be compliant with OECD Guideline for Testing of Chemicals No. 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test", referenced as Method C.3 of Commission Regulation No. 440/2008 amended byCommission Regulation (EU) 2016/266 and with the “Guidance document on aquatic toxicity testing of difficult substances and mixtures” (OECD No. 23).
In this 72 hour acute toxicity study, the cultures of P. subcapitata were exposed to test item at nominal concentrations 1, 3, 10, 31, 99 and 313 mg.L-1 under static conditions in accordance with the OECD 201. WAF preparation was performed to prepare the sample. The EL50 values based on growth rate was at 60.371 mg/L.
All the validity criteria were fulfilled. This toxicity study is classified as acceptable and satisfies the guideline requirements for OECD 201 toxicity study.
Results Synopsis
Test Organism: P. subcapitata
Test Type: static
72hr EL50: 60.371.mg/L
Endpoint(s) Effected: growth rate inhibition
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