Zinc acrylate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Deviations:

yes

Remarks:

see 'Principles of method if other than guideline'

Principles of method if other than guideline:

Comparable to guideline study with acceptable restrictions (Documentation of the older study falls short of today´s requirements, but corresponds to the standards at the time the study was conducted; the quality of the study performance is nevertheless not influenced. Sufficient data suitable for assessment.)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: Municipal activated sludge from laboratory wastewater treatment plant fed with municipal sewage and synthetic wastewater.
- Initial biomass concentration: Concentration of dry substance = 1 g/L

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Nominal and measured concentrations:

Nominal: control, 100, 500, 1000 mg/L

Details on test conditions:

- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

900 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Key result

Duration:

30 min

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC20 = 3 mg/L
EC50 = 10 mg/L
EC80 = ca. 150 mg/L

Conclusions:

Under the study conditions, the 3 min NOEC established based on inhibition of nitrification rate was 100 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the test substance to microorganisms according to ISO Method 8192. Municipal activated sludge was used as inoculum and initial biomass concentration of dry substance was at 1 g/L. Test substance concentration applied was: 100, 500, 1000 mg/L plus control. Under the study conditions, the 3 min NOEC established based on inhibition of nitrification rate was 100 mg/L (BASF, 1993).

Reference 2

Endpoint:

activated sludge nitrification inhibition testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Activated sludge from a municipal wastewater treatment plant

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

4 h

Test temperature:

20°C

pH:

6.5-7.5

Dissolved oxygen:

5-6 mg/L

Nominal and measured concentrations:

between 0-1.2 mg Zn/L.

Reference substance (positive control):

no

Key result

Duration:

4 h

Dose descriptor:

other: IC20

Effect conc.:

0.16 mg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

inhibition of nitrification rate

Key result

Duration:

4 h

Dose descriptor:

IC50

Effect conc.:

0.35 mg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

inhibition of nitrification rate

Key result

Duration:

4 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

inhibition of nitrification rate

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, measured IC50 and NOECs were established based on inhibition of nitrification rate. The 4 h IC50 was determined at 0.35 mg/L and the 4 h NOEC at 0.1 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the test substance to microorganisms according to ISO Method 9509. The experiments were performed in two isothermal, closed and well-mixed reactors, one as a reference of the non-inhibited system, and one with the inhibitor to be tested. Each reactor was filled with 400 mL of activated sludge collected from a municipal wastewater treatment plant at 3–3.5 g/L MLSS, 80 mL of substrate fluid. The concentration range of Zn2 + used was 0.3 to 1.2 mg/L. Under the study conditions, measured IC50 and NOECs were established based on inhibition of nitrification rate. The 4 h IC50 was determined at 0.35 mg/L and the 4 h NOEC at 0.1 mg/L (Juliastuti, 2003).

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

0.1 mg/L

Additional information

Zinc sulphate studies

A study was conducted to determine the toxicity of the test substance to microorganisms according to ISO Method 9509. The experiments were performed in two isothermal, closed and well-mixed reactors, one as a reference of the non-inhibited system, and one with the inhibitor to be tested. Each reactor was filled with 400 mL of activated sludge collected from a municipal wastewater treatment plant at 3–3.5 g/L MLSS, 80 mL of substrate fluid. The concentration range of Zn2 + used was 0.3 to 1.2 mg/L. Under the study conditions, measured IC50 and NOECs were established based on inhibition of nitrification rate. The 4 h IC50 was determined at 0.35 mg/L and the 4 h NOEC at 0.1 mg/L (Juliastuti, 2003).

Acrylic acid studies

A study was conducted to determine the toxicity of the test substance to microorganisms according to ISO Method 8192. Municipal activated sludge was used as inoculum and initial biomass concentration of dry substance was at 1 g/L. Test substance concentration applied was: 100, 500, 1000 mg/L plus control. Under the study conditions, the 3 min NOEC established based on inhibition of nitrification rate was 100 mg/L (BASF, 1993).