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EC number: 200-345-9 | CAS number: 57-67-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 1998
- Deviations:
- yes
- Remarks:
- Medium: ADaM; feed: S. dimorphus 8x10^5 cells/mL
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Sulfaguanidine (minimum purity: 99%) was purchased from Sigma–Aldrich.
- Analytical monitoring:
- yes
- Details on sampling:
- Sublethal effects on growth were determined as follows. Sixty haphazardly selected juveniles (<24 h old) were transferred in clean ADaM, fixed by progressively adding ethanol to a 70% concentration, and their length measured (from the top of the eye to the base of the tail spine) by means of a DMD108 digital microimaging device (Leica Microsystems, Milano, Italy). The same procedure was repeated on the surviving daphnids at the end of tests and the average daily growth rate calculated over the 21-days period.
- Vehicle:
- yes
- Remarks:
- ADaM medium
- Details on test solutions:
- Fresh stock solutions were prepared just before each experiment by diluting in the proper medium (ADaM) the compound to be tested. Where necessary, the solubilization was achieved by bringing back the pH of the medium to the original value using 1 M NaOH. The pH was measured using a BASIC 20 pH meter (CRISON, Carpi, Italy). All working solutions used in tests were prepared by appropriately diluting the stock solution with medium to the requested concentration.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna, <24-h old
- Strain/clone: Straus
- Source:from a single clone, cultured and maintained in ADaM medium and obtained from the second to fifth brood
FEEDING DURING TEST:
- Food type: S. dimorphus
- Amount: 8 x 10^5 cells mL-1
- Frequency: every other day - Test type:
- semi-static
- Water media type:
- other: ADaM medium
- Total exposure duration:
- 21 d
- Hardness:
- 193 mg CaCO3 L-1
- Test temperature:
- 20 ± 1°C
- pH:
- 8.0–8.3
- Dissolved oxygen:
- 6.2–6.8 mg/L
- Salinity:
- not specified
- Conductivity:
- 1016–1170 µS/cm
- Nominal and measured concentrations:
- As the concentration of all the tested substances was maintained within ±20% of the initial concentration, test results were based on nominal values. The nominal values were: control, 0.098, 0.195, 0.395, 0.781 and 1.562 mg/L.
- Details on test conditions:
- The ADaM medium was used for the controls and the dilution of test solutions. For each of the five concentration tested and for the control, 10 young daphnids were allocated individually in 50 mL of solution and incubated at the same conditions (light, temperature) used for culturing. During the tests, solutions were renewed every other day, the neonates removed and counted, and the feed (S. dimorphus, 8 x 10^5 cells/mL) supplied. Old (48-h) solutions from each concentration level were then pooled and monitored for pH, conductivity and dissolved oxygen using YSI 85 multiparameter instrument (YSI Incorporated, Yellow Springs, OH, USA).
- Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.395 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.781 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- other: EC20
- Effect conc.:
- 0.322 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.869 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- Statistically significant effects were observed on reproduction at concentrations of sulfaguanidine (SGD) equal or above 0.781 mg L-1 and these were concentration dependent. At concentration of 1.562 mg/L significant effects on growth were also observed. No mortality was observed in the control, with an average of 97 neonates per survived female.
CONFIDENCE INTERVALS (95% confidence level):
EC20: 0.152 - 0.559 mg/L
EC50: 0.630 - 1.097 mg/L - Results with reference substance (positive control):
- None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item sulfaguanidine (SGD) to Daphnia magna was determined in a 21-day test, resulting in a 21-day 0.395 mg/L NOEC and 0.781 mg/L LOEC.
EC20 was calculated to be 0.3217 mg/L while EC50 was calculated to be 0.869 mg/L. - Executive summary:
The aquatic toxicity of sulfaguanidine (SGD) was evaluated on Daphnia magna reproduction in general accordance with OECD guideline 211 of 1998. Statistically significant effects were observed on reproduction at concentrations equal or above 0.781 mg/L and these were concentration dependent. At concentration of 1.562 mg/L significant effects on growth were also observed. Based on results from statistical analysis, 0.781 mg/L was taken as the LOEC and 0.395 mg/L was taken as the NOEC. The estimated EC50 of SGD for D. magna reproduction was 0.87 mg/L.
Reference
Nominal concentration (mg L-1) |
Survival rate (%) |
Average daily growth (length, µm) |
Total number of neonates |
Neonates per female (mean ± sd) |
1.562 |
20 |
125.2 a |
17 |
8.50 a |
0.781 |
90 |
147.7 ± 13.8 |
499 |
55.44 ± 31.70 a |
0.395 |
80 |
145.5 ± 10.3 |
603 |
75.38 ± 36.79 |
0.195 |
100 |
154.3 ± 6.5 |
806 |
80.60 ± 30.63 |
0.098 |
100 |
152.9 ± 11.2 |
928 |
96.90 ± 16.86 |
Control |
100 |
156.6 ± 10.6 |
974 |
97.40 ± 14.23 |
a Significantly different from the control (p< 0.05). |
Description of key information
The aquatic toxicity of sulfaguanidine (SGD) was evaluated on Daphnia magna reproduction in general accordance with OECD guideline 211 of 1998. Statistically significant effects were observed on reproduction at concentrations equal or above 0.781 mg/L and these were concentration dependent. At concentration of 1.562 mg/L significant effects on growth were also observed. Based on results from statistical analysis, 0.781 mg/L was taken as the LOEC and 0.395 mg/L was taken as the NOEC. The estimated EC50 of SGD for D. magna reproduction was 0.87 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.395 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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