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EC number: 600-254-8 | CAS number: 10195-54-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations and the control
- Sample storage conditions before analysis: In pre-experiments significant losses of test article were determined in deep-frozen test medium samples. Therefore, all samples were analyzed immediately after sampling without prior storage. - Vehicle:
- yes
- Remarks:
- Reconstituted test water
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test medium of the highest test concentration of nominal 100 mg/L was prepared by dissolving 482 mg test article in 3 liters test water by intense stirring and short ultrasonic treatment. The amount of test article required was adjusted based on its purity (62.2%). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Clone: Straus
- Source: University of Sheffield/UK in 1992
- Age of animals: 6 - 24 h (not first brood progeny)
- Feeding during test: no
ACCLIMATION
not necessary, breed under test conditions - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 21 - 22 °C
- pH:
- 7.5 - 8.1
- Dissolved oxygen:
- 8.2 - 8.4 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 4.6, 10, 22, 46 and 100 mg/L
Measured concentrations: 3.6, 7.4, 17.6, 40 and 94 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: n100 mL glass beaker
- Material, fill volume: glass, 50 mL
- Aeration: untill oxygen saturation was reached before test, no aeration during test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST WATER PARAMETERS
- Preparation of dilution water: analytical grade salts were added to purified water to obtain the following nominal concentrations:
CaCI2 x 2H20: 2.0 mmol/L (= 294.0 mg/L)
MgS04 x 7H20: 0.5 mmol/L (= 123.0 mg/L)
NaHC03: 0.75 mmol/L (= 65.0 mg/L)
KCI: 0.075 mmol/L (= 5.8 mg/L)
Water Hardness Alkalinity: 2.5 mmol/L (= 250.0 mg/l) as CaC03 0.8 mmol/L
Ratio of Ca : Mg = 4 :1 (based on molarity)
Na : K =10:1 (based on molarity)
OTHER TEST CONDITIONS
- Adjustment of pH: yes, for the nominal concentrations of 22, 46 and 100 mg/L adjustment with 0.1M (NaOH) to values between 8.0 and 8.1
- Photoperiod: 16-hour light to 8-hour darkness
- Light intensity: 200 - 1200 Lux
EFFECT PARAMETERS MEASURED
Immobility of daphnids tested after 24 and 48h, pH and Oxygen after 0 and 48 h
RANGE-FINDING STUDY
- Test concentrations: not available
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 %CI: 11 - 14 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 40 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Effect concentrations exceeding solubility of substance in test medium: no - Reported statistics and error estimates:
- The 24-hour and 48-hour EC50 and the 95% confidence limits were calculated by Moving average interpolation. The NOEC, ECO and EC100 were determined directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to OECD 202 the test item showed a EC50 of 13 mg/L.
- Executive summary:
The acute toxicity of the test substance was evaluated with Daphnia magna STRAUS using a 48-hour static test according to OECD guideline No. 202 and EU method C.2. A preliminary test preceded the definitive test. Five concentrations of the test substance were used together with a control at: 4.6, 10, 22, 46 and 100 mg/L. Twenty daphnids (in two replicates of ten) were exposed to each concentration for 48 hours and immobilization was recorded at 0, 24 and 48 hours. Initial concentrations of the test item measured in the definitive test solutions were 69% till 82 % of the corresponding nominal values. Therefore, the reported biological results are related to the total mean measured test article concentrations: 3.6, 7.4, 17.6, 40 and 94 mg/L. Under the experimental conditions, the 48-hour EC50 of the test substance in a static test system is 13 mg/L (95% CI: 11 - 14 mg/L) for Daphnia magna.
Reference
Description of key information
According to OECD 202 the test item showed a EC50 of 13 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 13 mg/L
Additional information
The acute toxicity of the test substance was evaluated with Daphnia magna STRAUS using a 48-hour static test according to OECD guideline No. 202 and EU method C.2. A preliminary test preceded the definitive test. Five concentrations of the test substance were used together with a control at: 4.6, 10, 22, 46 and 100 mg/L. Twenty daphnids (in two replicates of ten) were exposed to each concentration for 48 hours and immobilization was recorded at 0, 24 and 48 hours. Initial concentrations of the test item measured in the definitive test solutions were 69% till 82 % of the corresponding nominal values. Therefore, the reported biological results are related to the total mean measured test article concentrations: 3.6, 7.4, 17.6, 40 and 94 mg/L. Under the experimental conditions, the 48-hour EC50 of the test substance in a static test system is 13 mg/L (95% CI: 11 - 14 mg/L) for Daphnia magna.
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