Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Hazard for aquatic organisms

Freshwater

Hazard assessment conclusion:
PNEC aqua (freshwater)
PNEC value:
27.2 µg/L
Assessment factor:
1 000
Extrapolation method:
assessment factor
PNEC freshwater (intermittent releases):
272 µg/L

Marine water

Hazard assessment conclusion:
PNEC aqua (marine water)
PNEC value:
27.2 µg/L
Assessment factor:
10 000
Extrapolation method:
assessment factor

STP

Hazard assessment conclusion:
PNEC STP
PNEC value:
65 000 µg/L
Assessment factor:
1
Extrapolation method:
assessment factor

Sediment (freshwater)

Hazard assessment conclusion:
no exposure of sediment expected

Sediment (marine water)

Hazard assessment conclusion:
no exposure of sediment expected

Hazard for air

Air

Hazard assessment conclusion:
no hazard identified

Hazard for terrestrial organisms

Soil

Hazard assessment conclusion:
PNEC soil
PNEC value:
3.6 µg/kg soil dw
Extrapolation method:
equilibrium partitioning method

Hazard for predators

Secondary poisoning

Hazard assessment conclusion:
no potential for bioaccumulation

Additional information

Arabinofuranosidase was tested for aquatic toxicity in the algal growth inhibition assay and the daphnia immobilisation assay. No fish test was performed, but read-across to alpha-amylase an enzyme belonging to the same sub-subclass was applied for this end point.

For fish: Alpha-amylase was not toxic at the the tested concentration of 100 mg/L (58.3 mg aep/L) and thus the NOEC was determined to be 100 mg/L (58.3 mg aep/L). The LC50 value could not be determnined due to the absence of toxicity of the test compound at the tested concentration. Since the two enzymes belong to the same enzyme subsubclass, similar results can be expected for the arabinofuranosidase.

For crustacean Daphnia magna: Under the conditions of the test, arabinofuranosidase, batch PPH40331 was found not to be toxic to Daphnia magna at a nominal concentration up to 100 mg TOS/L (27.2 mg aep/L) after 48h exposure. Thus, no 48h EC50 could be calculated and must be > 100 mg TOS/L (> 27.2 mg aep/L). The 48 h EC50 value for alpha-amylase with Daphnia magna was 2000 mg test material/L corresponding to 212 mg active enzyme protein/L.

For algae: Arabinofuranosidase, batch PPH40331 up to a concentration of 100 mg/L (27.2 mg aep/L) was not toxic to Pseudokirchneriella subcapitata as no significant growth inhibition was observed in the present test system. Thus no 72h ErC50 could be calculated and must be > 100 mg TOS/L  (> 27.2 mg aep/L).

Conclusion on classification

Based on the aquatic toxicity studies and the ready biodegradation of the enzyme, arabinofuranosidase is not classified.