Castor oil, sulfated

Endpoint summary

Description of key information

Short term toxicity to fish:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Castor oil, sulfated on Zebra fish (Danio rerio) was determined to be 25 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. As the chemical was readily degradable in water thus before causing any toxicity on the fishes it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

1. As the chemical was not highly soluble in water thus test conducted at 10 mg/l. After the exposure of 48 hrs, only 4 % immobility were observed in Daphnia magna. Thus on that criteria chemical was not consider as toxic. Based on the study chemical was may be consider as nontoxic and notclassified as per the CLP criteria.

2. Based on the immobility of daphnia magna by providing the exposure of test chemical for 48 hrs, the EC50 was determine to be > 5 mg/l.

On the basis of the experimental studies and all the data summarized for Castor oil, sulfated (8002-33-3), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Toxicity to aquatic algae:  

Based on the growth inhibition of green alga Chlorella vulgaris by the test chemical Castor oil, sulfated, the EC50 was determine to be > 200 mg/l. Based on the EC50, it can be concluded that the chemical Castor oil, sulfated was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Additional information

Summarized result for the toxicity of test chemical on the growth of aquatic life are as follows:

Short term toxicity to fish:

The key study was used from the experimental data 2018 in which toxicity was measured. This study was designed to assess the toxic effects of the test compound Castor oil, sulfated on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Castor oil, sulfated on Zebra fish (Danio rerio) was determined to be 25 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. As the chemical was readily degradable in water thus before causing any toxicity on the fishes it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity to aquatic invertebrates of the test chemical Castor oil, sulfated. The studies are as mentioned below: 

 

Study from experimental study report 2017, Aim of this study was to assess the short term toxicity of to test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline 202 in a static system for the total exposure period of 48 hrs. The stock solution 10 g/l was prepared by dissolving white powder in acetone. For a final concentration of 100 mg/l in the test solution the final stock solution was mixed in reconstitute water.  It was possible to test at higher substance concentration due to the formation of precipitate. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. As the chemical was not highly soluble in water thus test conducted at 10 mg/l. After the exposure of 48 hrs, only 4 % immobility were observed in Daphnia magna. Thus on that criteria chemical was not consider as toxic. Based on the study chemical was may be consider as nontoxic and not classified as per the CLP criteria.

 

Similarly another study from authoritative database was conducted to evaluate the effect of test chemical on the mobility of daphnia magna by providing the exposure period of 48 hrs. Test conducted in accordance with the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Study performed under the static system. Based on the immobility of daphnia magna by providing the exposure of test chemical for 48 hrs, the EC50 was determine to be > 5 mg/l. As the chemical was readily degradable in water thus before causing any toxicity on the daphnia and other aquatic invertebrates it degrades. Thus on the overall criteria chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

On the basis of the experimental studies and all the data summarized for Castor oil, sulfated (8002-33-3), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Toxicity to aquatic algae: 

The study from experimental report 2018 was designed to assess the toxic effects of the test compound Castor oil, sulfated on the green alga Chlorella vulgaris. Test was conducted in compliance with the OECD guideline 201 (Alga, Growth Inhibition Test). All the tests were carried out in 100mL conical flasks which were carefully autoclaved and sterilized. The test solution in each of these test vessels was kept constant which is 60 ml so that a sufficient amount of head space was left. The test item Castor oil, sulfated was prepared by adding 50 mg of test item in 250 ml of BBM to get the final concentration of 200mg/L. This stock solution was kept for stirring/ sonication for 0 minutes to obtain a homogenous solution for the experiment. The test concentrations 6.25mg/l,12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200 mg/l all the six concentration were in geometric series spaced by a factor of 2 were chosen according to the available data of the test item. The concentrations chosen were set up to the water solubility limit. The remaining test solutions were prepared by dilution from the above stock solution. For the assessment of algal growth, the test was conducted in replicates. The control flask was maintained in triplicates as recommended in the OECD guideline and the test concentration were selected in geometric series which were maintained in duplicates. To obtain a quantitative concentration-response relationship by regression analysis, a linearizing transformation of the response data into probit was performed. Using the same, effective concentration (EC) were determined. Algal growth was calculated daily by counting the cells microscopically with the help of haemocytometer. For microscopic observations the cultures were observed daily with the help of a microscope to verify a normal and healthy appearance of the algal culture and also to observe any abnormal appearance of the algae (as may be caused by the exposure of the test item). Apart from this, the cell count of each test vessel was also noted with the help of a microscope and haemocytometer. By spectrophotometer the absorbance values of each test vessel and control vessel was noted at 680nm.The BBM was taken as blank for both control and test vessels. The absorbance value of each vessel was in line with the average specific growth rate. As per OECD 201, the biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72 hr test period. This corresponds to a specific growth rate of 0.92 per day. Thus, the observed specific growth rate in the control cultures during the experiment was 0.358 per day. Secondly the mean coefficient of variation for section by section specific growth rates (days 0-1, 1-2 & 2-3, for 72 hr tests) in the control cultures must not exceed 35%. Thus, the observed mean coefficient of variation in the control cultures during the experiment was 33.42%. Thirdly the coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 10%. Thus, the observed coefficient of variation of average specific growth rates during the experiment in control cultures was 8.26%. Hence, the test is considered valid as per OECD guideline, 201. Based on the growth inhibition of green alga Chlorella vulgaris by the test chemical Castor oil, sulfated, the EC50 was determine to be > 200 mg/l. Based on the EC50, it can be concluded that the chemical Castor oil, sulfated was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Based on the all experimental studies for castor oil, sulfated (8002-33-3), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.