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EC number: 269-304-0 | CAS number: 68214-62-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From April 06th to September 27th, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Justification for type of information:
- The read across approach is detailed into the document attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Version / remarks:
- May 12, 1981
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Microorganisms (activated bacteria sludge) from a domestlc waste-water sewage plant (ARA, Sissach/Switzerland).
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Sliudge amount: an amount of sludge from a domestic waste-water sewage plant corresponding to 7.5 mg dry material for the positive control and 25.0 mg dry material for the test article were added to 250 ml of the flnal test medium.
- Temperature: 22.5 - 25 °C
- Continuous darkness: flasks incubated protected from light.
TEST MEDIUM
Stock solution:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 44.6 g Na2HPO4 × 12H2O, 1.7 g NH4Cl dissolved in 1000 ml bidistllled water.
b) 22.5 g MgSO4 × 7H2O dissolved in 1000 ml water.
c) 27.5 g CaCl2 dissolved in 1000 ml water.
d) 0.25 g FeCl3 × 6H2O dissolved in 1000 ml water.
Three ml each of the stock solutlons (a-d) were added to 1000 ml bidistilled water (final test medium pH adjusted to 7.0).
CONTROL AND BLANK SYSTEM
- Inoculum blank: untreated inoculated control was run.
SAMPLEN PREPARATION
At the end of the incubation period, samples of at least 10 ml from all flasks were centrifuged and thereafter directly analysed. - Reference substance:
- aniline
- Remarks:
- 100 mg/l; ThOD of 241 mg O2 per 100 g aniline
- Parameter:
- other: % BOD/COD
- Value:
- 23.8
- Sampling time:
- 28 d
- Parameter:
- other: % BOD/DOC
- Value:
- 31.8
- Sampling time:
- 28 d
- Results with reference substance:
- Degradation (28d): 73.2 % (BOD/ThOD).
- Interpretation of results:
- other: possible inherently biodegradable
- Conclusions:
- The test article was degradable to 23.8 %, based on BOD/COD assay, or 31.8 %, based on BOD/DOC assay, within 28 days.
- Executive summary:
The biodegradability of test item exposed to activated sludge of a municipal sewage treatment plant was investigated under aerobic conditions. The procedures outlined into the OECD guideline 302 C were followed.
The biodegradation of the test article was determined by means of the biochemical oxygen demand (BOD) for 28 days. Results obtained were compared with those obtained with aniline tested simultaneously under the same conditions.
The test article was degradable to 23.8 %, based on BOD/COD assay, or 31.8 %, based on BOD/DOC assay, within 28 days.
The standard compound, aniline, was degraded within 28 days to 73.2 %, based on BOD/ThOD.
Conclusion
The test article was degradable to 23.8 %, based on BOD/COD assay, or 31.8 %, based on BOD/DOC assay, within 28 days.
Reference
Biodegradation of test item in activated sludge determined by means of DOC
Flask | mg DOC/l after 28 days | % BD | |
Test item flask 1 | sb | 2.2 | - |
Test item flask 2 | sa | 1.5 | 31.8 |
Test item flask 3 | sa | 1.4 | 36.4 |
Test item flask 4 | sa | 1.6 | 27.3 |
Mean | - | 31.8 |
sa = remaining amount of test article after 28 days by means of DOC-analyses (mg)
sb = remaining amount of test article after 28 days in the control flask test article without inoculum.
Biochemical oxygen demand of test item and aniline
Concentration mg test article per 250 ml | mg Hg* after | BOD mg O2/100 ml test article after | % BD | |||
14 d | 28 d | 14 d | 28 d | BOD/COD (28d) | BOD/ThOD (28d) | |
**Test item 7.5 | 0 | 0 | 0 | 0 | 0 | - |
Test item 7.5 | 0 | 2.5 | 0 | 41.7 | 34 | - |
Test item 7.5 | 1 | 1 | 16.7 | 16.7 | 13.6 | - |
Mean | - | - | - | - | 23.8 | - |
Aniline 25 | 37.5 | 38.5 | 187.5 | 192.5 | - | 79.9 |
Aniline 25 | 32 | 32 | 160 | 160 | - | 66.4 |
Mean | - | - | - | - | - | 73.2 |
* Corrected for control value (0 - 4.5 mn Hg) on the respective day.
** Flask leakage
Description of key information
Not readily biodegradable.
Key value for chemical safety assessment
Additional information
Acid Blue 280 is an anthraquinone derivative; it is not expected to be ready biodegradable because of its chemical structure and its specific function. Commonly, dyes undergo a primary transformation, i.e. discolouration due to the interruption of the conjugation. Nevertheless, the degradation process involves more steps and take more time.
The assumption above mentioned is confirmed by the experimental data available on the structural analogous Similar Substance 01. The read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).
The biodegradability of Similar Substance 01 was investigated according to the testing procedures outlined into the OECD guideline 302 C. The test article was degradable to 23.8 %, based on BOD/COD assay, or 31.8 %, based on BOD/DOC assay, within 28 days.
On the basis of the curve shape, a lag-phase of 7 - 15 days can be observed, followed by a period of ca 6 days during which the substance reached the ca. 24 % of degradation. Then, from ca. the 21st up to the 28th day, the curve described a plateau.
Similar Substance 01 is deemed to be non-readily biodegradable. Although, biodegradation above 20 % of theoretical may be regarded as evidence of inherent, primary, biodegradability, a clear conclusion cannot be traced on the basis of the available information.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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