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EC number: 232-266-0 | CAS number: 7803-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on three toxicity to microorganism studies as-
WoE 2., WoE 3 and WoE 4. - GLP compliance:
- not specified
- Analytical monitoring:
- no
- Remarks:
- WoE 2 and WoE 3: not specified and WoE 4: no
- Vehicle:
- not specified
- Test organisms (species):
- other: WoE 2: Microcystis aeruginosa, WoE 3 and WoE 4: Chlorella vulgaris
- Details on test organisms:
- WoE 2:
TEST ORGANISM
- Common name: green algae
- Strain: FACHB 315
WoE 3:
TEST ORGANISM
- Common name: green algae
- Strain: A-23
- Method of cultivation: The culture was centrifuged, the harvested cells washed with distilled water and resuspended in medium without nitrogen.
WoE 4:
TEST ORGANISM
- Common name: green algae - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 120 h
- Remarks on exposure duration:
- WoE 2: 96 hrs and WoE 3 & WoE 4: 120 hrs
- Test temperature:
- WoE 2: 25°C
WoE 3 and 4: 26°C - pH:
- WoE 2: 8.3
WoE 3: 7.0
WoE 4: no data available - Details on test conditions:
- WoE 2: no data available
WoE 3:
TEST SYSTEM
- Aeration: Test culture was aerated with air containing 3% CO2.
GROWTH MEDIUM
- Standard medium used: Modified Prat's mineral medium was used as a test medium. Composition of the medium includes KH2PO4-0.135g, MgSO4.7H2O-0.5g, FeSO4.7H2O-0.003g, Sodium Citrate-0.006g, micronutrients solution-1ml, H2O-1000ml, respectively.
OTHER TEST CONDITIONS
- Light intensity: 6000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : After a period of 120 hrs, number of cells, percentage of living cells, average cells size and pH was measured. - Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 199.79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: WoE 2: 95% C. I. = 153.574 to 259.969 mg/l
- Duration:
- 120 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 1 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: WoE 3
- Duration:
- 120 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 5 080 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality
- Remarks on result:
- other: WoE 3
- Duration:
- 120 h
- Dose descriptor:
- IC50
- Effect conc.:
- 330 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: WoE 4
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the comparatively similar read across chemical and applying the weight of evidence approach, the 96 EC50 and 120 hr EC50/LC50/IC50 value of the test chemical on test organism can be expected to be 199.79 mg/l and in the range of 330 to 5080 mg/l, respectively.
- Executive summary:
Data available for the comparatively similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic algae. The studies are as mentioned below:
An acute toxicity test was conducted for 96 hrs for assessing the effect of test chemical on green algae. Microcystis aeruginosa FACHB 315 (green algae) was used as a test organism. The study was performed taking cells from an exponential growth phase culture in a static system at a temperature of 25°C and pH 8.3, respectively. On the basis of effect of test chemical on decrease in growth rate of the test organism, the 96 hr ErC50 value was determined to be 199.79 mg/l (95% C. I. = 153.574 to 259.969 mg/l).
Another acute toxicity test was conducted for 120 hrs for assessing the effect of test chemical on green algae.Chlorella vulgarisA-23 (green algae) was used as a test organism. Study was performed in a static system. Modified Prat's mineral medium was used as a test medium. Composition of the medium includes KH2PO4-0.135g, MgSO4.7H2O-0.5g, FeSO4.7H2O-0.003g, Sodium Citrate-0.006g, micronutrients solution-1ml, H2O-1000ml, respectively. The culture was centrifuged, the harvested cells washed with distilled water and resuspended in medium without nitrogen. Test culture was aerated with air containing 3% CO2. 200ml aliquots were distributed into culture vessels containing appropriate amounts of test chemical as a nitrogen source. These test vessels were incubated for 120 hrs (5 days) under test conditions i.e, at a temperature of 26°C, pH 7.0 with light intensity of 6000 lux. After an exposure period of 120 hrs, number of cells, percentage of living cells, average cells size and pH was measured. On the basis of the effect on growth rate and mortality of the test organism, the 120 hr ErC50 and LC50 was determined to be 1300 mg/l and 5080 mg/l.
For the test chemical, toxicity to aquatic algae study was conducted for 120 hrs for assessing the effect of test chemical. Chlorella vulgaris(green algae) was used as a test organism. Study was performed in a static system at a temperature of 26°C. On the basis of the effect on growth rate of the test organism, the 120 hr IC50 value was determined to be 330 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 96 EC50 and 120 hr EC50/LC50/IC50 value of the test chemical on test organism can be expected to be 199.79 mg/l and in the range of 330 to 5080 mg/l, respectively. Thus, based on these values, test chemical was considered as non-toxic to aquatic algae and hence, considered to be ‘not classified’ as per the CLP classification criteria.
Reference
Description of key information
On the basis of the experimental studies of the comparatively similar read across chemical and applying the weight of evidence approach, the 96 EC50 and 120 hr EC50/LC50/IC50 value of the test chemical on test organism can be expected to be 199.79 mg/l and in the range of 330 to 5080 mg/l, respectively
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 199.79 mg/L
Additional information
Data available for the comparatively similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic algae. The studies are as mentioned below:
An acute toxicity test was conducted for 96 hrs for assessing the effect of test chemical on green algae. Microcystis aeruginosa FACHB 315 (green algae) was used as a test organism. The study was performed taking cells from an exponential growth phase culture in a static system at a temperature of 25°C and pH 8.3, respectively. On the basis of effect of test chemical on decrease in growth rate of the test organism, the 96 hr ErC50 value was determined to be 199.79 mg/l (95% C. I. = 153.574 to 259.969 mg/l).
Another acute toxicity test was conducted for 120 hrs for assessing the effect of test chemical on green algae. Chlorella vulgaris A-23 (green algae) was used as a test organism. Study was performed in a static system. Modified Prat's mineral medium was used as a test medium. Composition of the medium includes KH2PO4-0.135g, MgSO4.7H2O-0.5g, FeSO4.7H2O-0.003g, Sodium Citrate-0.006g, micronutrients solution-1ml, H2O-1000ml, respectively. The culture was centrifuged, the harvested cells washed with distilled water and resuspended in medium without nitrogen. Test culture was aerated with air containing 3% CO2. 200ml aliquots were distributed into culture vessels containing appropriate amounts of test chemical as a nitrogen source. These test vessels were incubated for 120 hrs (5 days) under test conditions i.e, at a temperature of 26°C, pH 7.0 with light intensity of 6000 lux. After an exposure period of 120 hrs, number of cells, percentage of living cells, average cells size and pH was measured. On the basis of the effect on growth rate and mortality of the test organism, the 120 hr ErC50 and LC50 was determined to be 1300 mg/l and 5080 mg/l.
For the test chemical, toxicity to aquatic algae study was conducted for 120 hrs for assessing the effect of test chemical. Chlorella vulgaris(green algae) was used as a test organism. Study was performed in a static system at a temperature of 26°C. On the basis of the effect on growth rate of the test organism, the 120 hr IC50 value was determined to be 330 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 96 EC50 and 120 hr EC50/LC50/IC50 value of the test chemical on test organism can be expected to be 199.79 mg/l and in the range of 330 to 5080 mg/l, respectively. Thus, based on these values, test chemical was considered as non-toxic to aquatic algae and hence, considered to be ‘not classified’ as per the CLP classification criteria.
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