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EC number: 250-792-9 | CAS number: 31765-95-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- published in O.J. L 142 (2008)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: Saturn Grey LCGCAS: 31765-95-4Batch No.: 8010/2008Appearance: black powderStorage: dark dry room, closed container, at laboratory temperatureExpiration date: February 2018
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical determination of the test substance concentrations were performed at the beginning of test, before renewal of the test solutions, after renewal of the test solutions and at the end of the test. The samples for analysis were taken from the highest (100 mg/L) and the lowest test concentration (1 mg/L). All samples for analysis were delivered in transport box to analytical laboratory immediately after preparation and after each test period (0-24 hours and 24-48 hours). An analysis of samples was started immediately after delivery. All samples were stored at laboratory temperature. The analytical results showed that the test substance was stable in dilution water under current test conditions.
- Vehicle:
- no
- Details on test solutions:
- The stock solution of the test substance was prepared in the dilution water. 50 mg of the test substance was weighed into 500 mL of the dilution water for the preliminary semi-static test. The concentrations of solutions used in the preliminary semis-static test were obtained by dilution of the stock solution with dilution water. The exact concentrations were determined using HPLC.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM - Common name: Daphnia magna - Strain/clone: Straus - Source: own laboratory breeding - Age: max. 24 hours
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 20±2°C, with a variation of ± 1°C at each individual test
- pH:
- 7.7 - 8.3
- Dissolved oxygen:
- 7.0 - 7.7 mg/L
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L 0 hours: 94.8 mg/L 24 hours (old): 93.8 mg/L 24 hours (new): 95.4 mg/L 48 hours: 96.5 mg/LNominal concentration: 1 mg/L 0 hours: 1.0 mg/L 24 hours (old): 1.0 mg/L 24 hours (new): 1.0 mg/L 48 hours: 0.8 mg/L
- Details on test conditions:
- TEST SYSTEM - Test vessel: laboratory glass beaker - Renewal rate of test solution (frequency/flow rate): 24 hours - No. of organisms per vessel: 10 - No. of vessels per concentration (replicates): 2 - No. of vessels per control (replicates): 2 TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: M4 medium OTHER TEST CONDITIONS - Adjustment of pH: no - Photoperiod: daylight
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- see Table 1
- Results with reference substance (positive control):
- Reference substance: K2Cr2O7EC50 = 1.17 mg/L (24 h)EC50 = 0.62 mg/L (48 h)Interlaboratory test: EC50 = 0.46 – 1.06 mg/L (48 h, K2Cr2O7)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The experiment with the concentration of 100 mg/L included in the preliminary semi-static test has been performed using 20 daphnia, therethrough the conditions of the limit test given in guidelines have been fulfilled. It was demonstrated that the EC50 is higher than this concentration.No immobilisation was observed in the preliminary semi-static test. Therefore exact values of EC50 could not be calculated and the values of EC are given in the form of a range. The EC0 and EC100 values are identified by direct observation. There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.EC50 > 100 mg/L (24 h, nominal concentration)EC50 > 100 mg/L (48 h, nominal concentration)EC0 = 100 mg/L (24 h, nominal concentration)EC0 = 100 mg/L (48 h, nominal concentration)EC100 > 100 mg/L (24 h, nominal concentration)EC100 > 100 mg/L (48 h, nominal concentration)
- Executive summary:
The test substance, Direct Black 112, was tested in acute immobilisation test on Daphnia magna. The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in range of the test substance nominal concentrations 1 – 100 mg/L. The test was performed as semi-static; the test solutions were renewed after 24 hours. The test substance concentrations was measured at the beginning, after 24 hours (before and after renewal) and at the end of the test at the highest (100 mg/L) and the lowest concentration (1 mg/L). The analytical results from the preliminary semi-static test showed, that the test substance was stable in dilution water at conditions of the test. Based on no toxicity of the test substance found in preliminary semi-static test, the study was terminated. The experiment with the nominal concentration of 100 mg/L included in the preliminary semi-static test has been performed using 20 daphnia, therethrough the conditions of the limit test given in guideline have been fulfilled. It was demonstrated that the EC50is higher than this concentration.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
EC50> 100 mg/L (24 h, nominal concentration)
EC50> 100 mg/L (48 h, nominal concentration)
EC0= 100 mg/L (24 h, nominal concentration)
EC0= 100 mg/L (48 h, nominal concentration)
EC100> 100 mg/L (24 h, nominal concentration)
EC100> 100 mg/L (48 h, nominal concentration)
Reference
Table 1: Immobilisation of daphnia – preliminary semi-static test
Nominal concentration | mg/L | 100 | 50 | 10 | 5 | 1 | C |
Number of mobile animals | 24 hours/1stseries | 10 | 10 | 10 | 10 | 10 | 10 |
Number of mobile animals | 24 hours/2ndseries | 10 | 10 | 10 | 10 | 10 | 10 |
Number of mobile animals | 24 hours/total | 20 | 20 | 20 | 20 | 20 | 20 |
Immobilisation % | 24 hours | 0 | 0 | 0 | 0 | 0 | 0 |
Number of mobile animals | 48 hours/1stseries | 10 | 10 | 10 | 10 | 10 | 10 |
Number of mobile animals | 48 hours/2ndseries | 10 | 10 | 10 | 10 | 10 | 10 |
Number of mobile animals | 48 hours/total | 20 | 20 | 20 | 20 | 20 | 20 |
Immobilisation % | 48 hours | 0 | 0 | 0 | 0 | 0 | 0 |
Description of key information
The experiment with the nominal concentration of 100 mg/L included in the preliminary semi-static test has been performed using 20 daphnia, therethrough the conditions of the limit test given in guideline have been fulfilled. It was demonstrated that the EC50is higher than this concentration.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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