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EC number: 278-248-6 | CAS number: 75535-16-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-[2-[4-(diethylammonio)phenyl]vinyl]-1,3,3-trimethyl-3H-indolium oxalate
- EC Number:
- 281-588-8
- EC Name:
- 2-[2-[4-(diethylammonio)phenyl]vinyl]-1,3,3-trimethyl-3H-indolium oxalate
- Cas Number:
- 83969-11-3
- Molecular formula:
- C23H29N2.C2HO4
- IUPAC Name:
- 2-{2-[4-(diethylammonio)phenyl]vinyl}-1,3,3-trimethyl-3H-indolium oxalate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Basic Violet 16
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix from rat liver
- Test concentrations with justification for top dose:
- 1, 5, 10, 50, 100, 500 µg/plate
- Vehicle / solvent:
- water
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-Aminoanthracene; 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, 4-nitro-o-phenylenediamine
- Details on test system and experimental conditions:
- Bacterial strains
Salmonella typhimuriurn TA100, TA98, TA1535, TA1537 and TA1538 were obtained from Professor B.N. Ames, University of California, U.S.A.
Escherichia coli WP2 uvrA was provided by Dr. T. Kada, National Institute of Genetics, Mishima, Japan.
Cultures of each strain were prepared by incubating an aliquot of parent stock culture in nutrient broth at 37°C for 18 hours.
After mixing 0.1 ml of test material solution, 0.5 ml of phosphate buffer or S-9 mix, 0.1 ml of culture of tester strain, and 2 ml of top agar in a test tube, the mixture was poured on minimal agar plate. The plates were incubated at 37°C for 2 days and scored, for revertants.
For each concentrations 2 plates were prepared and scored.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Under the study conditions, the test substance was found to be not mutagenic in the bacterial reverse mutation assay.
- Executive summary:
A bacterial reverse mutation assay (Ames) was carried out, to evaluate the test substance for the mutagenic potential in a microbial assay with and without the addition of mammalian metabolic activation preparation. The test was carried out in strains of Salmonella typhimuriurn TA100, TA98, TA1535, TA1537 and TA1538 and Escherichia coli WP2 uvrA. Cultures of each strain were prepared by incubating an aliquot of parent stock culture in nutrient broth at 37°C for 18 hours. After mixing 0.1 ml of test material solution, 0.5 ml of phosphate buffer or S-9 mix, 0.1 ml of culture of tester strain, and 2 ml of top agar in a test tube, the mixture was poured on minimal agar plate. The plates were incubated at 37°C for 2 days and scored, for revertants.
Cytotoxicity was observed at concentations of 100 and 500 µg/plate. The test substance was tested negative with and without metabolic activation in all strains tested.
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