Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 279-506-0 | CAS number: 80584-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Results of the harmful effects of water pollutants to Daphnia magna in the 21 day reproduction test.
- Author:
- Kuehn R, Pattard M, Pernak KD and Winter A
- Year:
- 1 989
- Bibliographic source:
- Water Research 23(4): 501-510.
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Provisional proposal by German Federal Environment Agency: Extended toxicity test using Daphnia magna (determination of NOEC for reproduction rate, mortality and the time of appearance of first offspring; 01 Jan 1984)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: This internal test method of the German Federal Environment Agency is a precursor of the OECD guidelines for the Daphnia reproduction test (initial version: OECD 202, part II; recent version: OECD 211)
- Principles of method if other than guideline:
- German title: Umweltbundesamt: Vorläufiger Testverfahrensvorschlag: Verlängerter Toxizitätstest bei Daphnia magna (Bestimmung der NOEC für Reproduktionsrate, Mortalität und den Zeitpunkt des ersten Auftretens von Nachkommen; 21 d). Stand: 01.01.1984", gegeben als "Empfehlung des Umweltbundesamtes zur Durchführung der Prüfung nach § 5 Abs. 1 Nr. 3 der Verordnung über Anmeldeunterlagen und Prüfnachweise nach dem ChemG.
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,2',2''-nitrilotriethanol
- EC Number:
- 203-049-8
- EC Name:
- 2,2',2''-nitrilotriethanol
- Cas Number:
- 102-71-6
- Molecular formula:
- C6H15NO3
- IUPAC Name:
- 2,2',2''-nitrilotriethanol
- Details on test material:
- - Name of test material (as cited in study report): Triethanolamine
- Analytical purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling occasions: first sampling: on day of water exchange before 7th day (no offspring); second sampling: on day of water exchange between 16th and 21st day
- Concentrations: stock solution and selected dilution levels (in control and test concentrations without animals and food: fresh and aged after 48/72 h)
- Sampling method: not specified
- Sample storage conditions before analysis: not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution (2000 mg/L), stirred for 24 h, dilution water heated to 30 °C to facilitate the solution process
- Controls: negative control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: waterfleas
- Strain/clone: IRCHA
- Justification for species other than prescribed by test guideline: not applicable
- Source: The stock culture was kept since 1982 at the testing laboratory under the conditions described below.
- culture:
- 20-30 animals in 40 2-L beakers filled with >= 1.6 L tap water (hardness: 285.76 mg/L as CaCO3; pH 7.6-7.7; oxygen-saturated; water exchange: two times per week)
- beakers covered with watch glasses
- temperature: 20 °C
- fluorescent light (9 h light : 15 h dark)
- offspring removed daily from cultures
- feeding: dried algae (Scenedesmus sp.; suspension: 9 g dried algae/L, 2 mL/beaker)
- Age of parental stock (mean and range, SD): no data
- Age of test animals: <= 24 h
- Feeding during test
- Food type: fish feed (Tetramin Hauptfutter; suspension of 200 g/L; day 1 to 7: 1 mL; day 8 to 21: 1.5 mL) and activated sludge from municpal sewage treatment plant (0.1 mL per day = COD 10 mg/L ± 15%)
- Amount: 15-20 mg COD/L per day
- Frequency: 3 times per week
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 25±1 °C
- pH:
- >= 7.0 at end of test period
- Dissolved oxygen:
- average minimum oxygen saturation: 58%
- Nominal and measured concentrations:
- nominal: 8, 16, 32, 64, 128, 256, 512, 1000 mg/L (dilution ratio: 1:2)
measured: deviation < 20% - Details on test conditions:
- TEST SYSTEM
- Test vessel: bottles with ground glass stoppers
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 250 mL, no headspace, 250 mL
- Aeration: no (dilution water was oxygen-saturated prior to use)
- Renewal rate of test solution (frequency/flow rate): semistatic conditions (3 times/week)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 (one additional vessel was set up in order to replace animals without egg packets or very small animals until registration of first offspring)
- No. of vessels per control (replicates): 4 (one additional vessel was set up in order to replace animals without egg packets or very small animals until registration of first offspring)
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: 1 animal / 50 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to German Industrial Standard DIN 38412, Part I and Part II (1982): 11.76 g/l CaCl2*2H2O, 4.93 g/l MgSO4*7H2O, 2.59 g/l NaHCO3, 0.23 g/l KCl
- pH: 8.0±0.2
- Na:K ratio: 10:1
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 9 h light : 15 h dark
- Light intensity: fluorescent light (1.2 to 1.4 W/m²)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- observations: 3 times/week: dead/immobile animals, offspring, pH, dissolved oxygen
VEHICLE CONTROL PERFORMED: not applicable as no vehicle was used
RANGE-FINDING STUDY
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: 24-h EC0 = 1530 mg/L; 24-h EC50 = 2038 mg/L - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks:
- of parent animals
- Remarks on result:
- other: test concentration analytically verified (deviation < 20% from nominal value)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks:
- (reproduction rate)
- Remarks on result:
- other: test concentration analytically verified (deviation < 20% from nominal value)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks:
- (appearance of first offspring)
- Remarks on result:
- other: test concentration analytically verified (deviation < 20% from nominal value)
- Details on results:
- - Mortality of parent animals: 3.3% (9.1% based on 29 control runs)
- No. of offspring produced per day per female: 69 (68; SD = 10.6%; coefficient of variation = 14.8%; based on 29 control solutions)
- Time to first brood release or time to hatch: 7 days (7 days based on 29 control solutions)
- ACR ratio (24-h EC50 = 2038 mg/L) = 127 - Results with reference substance (positive control):
- - Results with reference substance valid? yes (potassium dichromate)
- Relevant effect levels: 24-h EC50 = 0.93 mg/L (range as stated in OECD TG 202: 0.6 to 2.1 mg/L) - Reported statistics and error estimates:
- Student t-test, u-test according to Sachs (1969) as well as graphic determination of dosis-effect curve for parental mortality, reproduction rate and appearance of first offspring
Any other information on results incl. tables
Parental mortality
- Control after 21 d: 3.3%
- Highest value not different from control value: 16 mg/L
- Lowest value different from control value: 32 mg/L (50%)
Reproduction rate
- Number of offspring per animal in control after 21 d: 69
- Highest value not different from control value: 125 mg/L
- Lowest value different from control value: 250 mg/L ( 59)
First appearance of offspring
- First day of appearance of offspring in control: 7
- Highest value not different from control value: 250 mg/L
- Lowest value different from control value: 500 mg/L (day 9)
Chemical analysis
The lowest retrieval was 78% (32 mg/L, aged test solutions, day 7), while the average value is 88% (range 78% to 96%). Therefore it can be concluded that the substance was stable over the exposure period. The measured test concentrations did not deviate by more than 20% from the nominal values, therefore effect values can be based on nominal values.
pH values
The dilution water had a pH of 8.0±0.1. The lowest value determined in the test solutions was 7.2. This range is not harmful to Daphnia
Dissolved oxygen
The lowest value determined during the 21-d test period was 55% (test solution of 32 mg/L). According to the recent OECD TG 211, the oxygen level should not fall below 60%. Nevertheless, this value can be tolerated by the Daphnia, a negative impact can be excluded on the reproductive behaviour of the test animals regarding the determined effect values.
Test solution |
Chemical analysis |
pH |
Dissolved oxygen |
||||||
(mg/L, nominal) |
initial |
day 7 |
day 21 |
day 7 |
day 21 |
day 7 |
day 21 |
||
fresh |
aged (2 d) |
fresh |
aged (2 d) |
||||||
0 (control) |
not stated |
||||||||
8 |
not stated |
||||||||
16 |
not stated |
7.5 |
7.2 |
64 |
65 |
||||
32 |
28/ 30 |
28 |
25 |
30 |
27 |
7.8 |
7.5 |
73 |
70 |
32* |
not stated |
26 |
28 |
not stated |
|||||
63 |
not stated |
52 |
55 |
not stated |
|||||
63* |
58/ 60 |
55 |
57 |
not stated |
|||||
125 |
115 |
120 |
not stated |
||||||
125* |
122/ 123 |
117 |
118 |
not stated |
|||||
250 |
not stated |
||||||||
500 |
not stated |
||||||||
1000 |
not stated |
||||||||
stock solution |
1995/ 1996 |
1994 |
|||||||
* blank vessels (without test animals and feed) |
Validity criteria
The validity criteria have been fulfilled.
- First offspring after < 10 d
- Reproduction rate > 40
- Parental mortality <= 20%
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.