Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 237-029-5 | CAS number: 13590-82-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15.08.2017 to 18.08.2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The cell numbers were determined at 24, 48 and 72 hours after start of exposure by manual cell counting using a microscope with counting chamber. The morphological changes of algal cells compared to the control were examined at 24, 48 and 72 hours after starting the test using a microscope.
- Vehicle:
- no
- Details on test solutions:
- The test solutions used in the test was prepared by mechanical dispersion without using any solubilising agents. The test item is poorly water soluble material. For preparation of the test solutions a supersaturated solution (100 mg/L nominal loading) was first prepared by dispersing/dissolving an amount of 0.1 g test item into 1000 mL test medium (OECD medium). This solution was agitated by orbital shaker overnight and then the non-dissolved test material was separated by filtration through a membrane filter (0.22 µm) in order to obtain the saturated test solution (i.e. 100 % v/v saturated solution). The pH of this stock solution was adjusted to that of the culture medium using 1N NaOH. The test solutions of subsequent lower test concentrations were prepared by appropriate diluting of this stock solution.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Raphidocelis subcapitata (Printz-Starr)
- Strain: 61.81 SAG (identical strains: CCAP 278/4; UTEX 1648; ATCC 22662)
- Source: SAG: Collection of Algal Cultures, Inst. Plant Physiology, University of Göttingen, Untere Karspüle 2, D-37073 Göttingen, Germany
- Method of cultivation: Small algal cultures that are regularly planted on agar are transferred to fresh medium at least once every two months under standardised conditions according to the test guidelines. Pre-culture was prepared with Algal Mineral Salts Culture Medium, incubated under the conditions of the test and used when still exponentially growing, normally after an incubation period of 2 - 4 days. - Test type:
- static
- Water media type:
- other: OECD medium
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- not specified
- Test temperature:
- Temperature in the flask ranged from 23.0 – 23.4 °C and within the climate chamber between 22.3 and 23.9 °C.
- pH:
- 7.52 - 9.37 (Control: 8.77 - 9.32)
- Dissolved oxygen:
- not specified
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- The following nominal concentrations were tested: 6.25, 12.5, 25, 50 and 100 % v/v saturated solution. The corresponding calculated geometric mean concentrations were: 0.298, 0.391, 0.555, 0.786 and 1.105 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml Erlenmeyer flask
- Type: covered with air-permeable stoppers
- Medium volume per flask: 100 mL
- Initial cells density: 10^4 cells/mL
- Control end cells density: 104×10^4 cells/mL (mean value)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (OECD medium according to OECD 201)
OTHER TEST CONDITIONS
- Adjustment of pH: pH of stock solution was adjusted to that of the culture medium using 1N NaOH.
- Photoperiod: continuously
- Light intensity and quality: 7984 lux, fluorescent lamps
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber
- Morphological Changes of Algal Cells: microscopy
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: yes
- Test concentrations: 0.1, 1, 10 and 100 mg/L plus a control
- Results used to determine the conditions for the definitive study: 32.5% growth inhibition at the highest concentration, no growth inhibition at 10 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, test period: 07 – 10 March 2017
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.658 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.507 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.786 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.391 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.555 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.298 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.541 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: calculated value for anhydrous substance
- Results with reference substance (positive control):
- The 72h ErC50: 0.99 mg/L (95 % confidence limits: 0.68 – 1.49 mg/L). The 72h EyC50: 0.59 mg/L (95 % confidence limits: 0.47 – 0.75 mg/L).
- Reported statistics and error estimates:
- For the determination of the LOEC and NOEC, ANOVA and Dunnett’s test (α = 0.05; 2-sided) was used and for the determination of the ECx values Probit analysis was used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 72-h algal growth inhibition test with Raphidocelis subcapitata the effects of Cerium(IV) sulfate tetrahydrate on the growth of Raphidocelis subcapitata was determined. The EC50 values for inhibition of growth rate (ErC50) was 0.658 mg/L (hydrated substance) and 0.541 mg/L (calculated value for anhydrous substance),and for inhibition of yield (EyC50) 0.507 mg/L (hydrated substance) and 0.417 mg/L (calculated value for anhydrous substance). The EC10 value was determined to be 0.462 mg/L (hydrated substance) and 0.380 mg/L (calculated value for anhydrous substance) based on growth rate (ErC10) and 0.346 mg/L (hydrated substance) and 0.284 mg/L (calculated value for anhydrous substance) based on yield (EyC10).The results are based on measured geometric mean test item concentrations.
- Executive summary:
The purpose of this study was to determine the effect of the test itemCerium(IV) sulfate tetrahydrateon the growth of a unicellular green algal speciesRaphidocelis subcapitata. Exponentially growing cultures of Raphidocelis subcapitatawere exposed to test item concentrations of 6.25, 12.5, 25, 50 and 100 % v/v saturated solution ((corresponding to 0.298, 0.391, 0.555, 0.786 and 1.105 mg/L (calculated geometric mean measured concentrations)).The algal growth in relation to a control culture was determined over a fixed test period of 72 hours. The EC50 values for inhibition of growth rate (ErC50) was 0.658 mg/L (hydrated substance) and 0.541 mg/L (calculated value for anhydrous substance), and for inhibition of yield (EyC50) 0.507 mg/L (hydrated substance) and 0.417 mg/L (calculated value for anhydrous substance). The EC10 value was determined to be 0.462 mg/L (hydrated substance) and 0.380 mg/L (calculated value for anhydrous substance) based on growth rate (ErC10) and 0.346 mg/L (hydrated substance) and 0.284 mg/L (calculated value for anhydrous substance) based on yield (EyC10). The results are based on measured geometric mean test item concentrations.
Reference
Description of key information
The effect of the test item on the growth of a unicellular green algal species Raphidocelis subcapitata was determined according to OECD 201, Regulation (EC) No. 226/2016 Method C.3 and EPA OPPTS 850.5400 and under GLP (reference 6.1.5-1). The study was conducted under static conditions with an initial cell density of 1x104 cells/mL for a period of 72 hours. The EC50 and EC10 based on growth rate were determined to be 0.658 mg/L (hydrated) and 0.541 mg/L ( calculated value for anhydrous substance), and 0.462 mg/L (hydrated substance) and 0.380 mg/L (calculated value for anhydrous substance) (mean measured), respectively.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 0.38 mg/L
Additional information
The effect of the test item on the growth of a unicellular green algal species Raphidocelis subcapitata was determined according to OECD 201, Regulation (EC) No. 226/2016 Method C.3 and EPA OPPTS 850.5400 and under GLP (reference 6.1.5-1). The study was conducted under static conditions with an initial cell density of 1x104 cells/mL for a period of 72 hours. A stock solution of 100 mg/L was freshly prepared with OECD Medium and shaken overnight and the non-dissolved test material was separated by filtration. Six concentrations were tested in a geometrical series with a dilution factor of 2: 6.25, 12.5, 25, 50 and 100 % v/v saturated solution ((corresponding to 0.298, 0.391, 0.555, 0.786 and 1.105 mg/L (calculated geometric mean concentrations)). The EC50 values for inhibition of growth rate (ErC50) was 0.658 mg/L (hydrated substance) and 0.541 mg/L (calculated value for anhydrous substance), and for inhibition of yield (EyC50) 0.507 mg/L (hydrated substance) and 0.417 mg/L (calculated value for anhydrous substance). The EC10 value was determined to be 0.462 mg/L (hydrated substance) and 0.380 mg/L (calculated value for anhydrous substance) based on growth rate (ErC10) and 0.346 mg/L (hydrated substance) and 0.284 mg/L (calculated value for anhydrous substance) based on yield (EyC10). The results are based on measured geometric mean test item concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.