Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-894-6 | CAS number: 625-45-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Study was performed before the implementation of OECD Guidelines, but followed in principle the test procedure described in OECD Guideline 401.
- GLP compliance:
- no
- Remarks:
- test predated GLP
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Methoxyacetic acid
- EC Number:
- 210-894-6
- EC Name:
- Methoxyacetic acid
- Cas Number:
- 625-45-6
- Molecular formula:
- C3H6O3
- IUPAC Name:
- 2-methoxyacetic acid
- Reference substance name:
- 2-methoxyacetic acid
- IUPAC Name:
- 2-methoxyacetic acid
- Details on test material:
- Name of test material:
Methoxyacetic acid
Impurities (identity and concentrations):
appr. 0.2 % formic acid
max. 0.5 % water
max. 0.1 % formaldehyde
< 0.1 % glycolic acid
< 0.1 % oxalic acid
< 0.5 % varied ester
< 0.001 % N
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Male rats: 180 - 200 g in weight.
Female rats: 160 - 170 g in weight.
Rats were fasted 15 - 20 hours prior to application.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- Concentration in vehicle (%): 6.81, 10.0, 14.7, 21.5, 31.6, 46.6
Application volume per dose: 10 mL/kg - Doses:
- 681, 1,000, 1,470, 2,150, 3,160 or 4,640 mg/kg bw
- No. of animals per sex per dose:
- 5 rats per sex and dose
- Control animals:
- no
- Details on study design:
- Several groups of 5 rats per sex and dose were treated by gavage with preparations of the test substance in water as vehicle. The concentrations of these preparations were usually adjusted to achieve a comparable volume of 10 mL per kg bw per animal. An observation period of 14 days was chosen.
Body weight was determined before the start of the study and 2-4, 7 and 13 days after dosing, too. Clinical signs were recorded and gross pathology was performed at the end of the observation period (day 14). - Statistics:
- On the basis of the observed lethality, the LD50 value was determined.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 1 000 - < 1 470 mg/kg bw
- Remarks on result:
- other: Level of significance: > 1000 mg/kg bw: 10%, < 1500 mg/kg bw: 1%
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 1 000 - < 1 470 mg/kg bw
- Remarks on result:
- other: Level of significance: 5 %
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 000 mg/kg bw
- Mortality:
- 681 mg/kg bw: males: 0/5; females: 0/5
1,000 mg/kg bw: males: 0/5; females: 2/5 (within 7 days)
1,470 mg/kg bw: males: 5/5 (within 2 days); females: 5/5 (within 2 days)
2,150 mg/kg bw: males: 5/5 (within 1 day); females: 5/5 (within 1 day)
3,160 mg/kg bw: males: 5/5 (within 1 day); females: 5/5 (within 1 day)
4,640 mg/kg bw: males: 5/5 (within 1 day); females: 5/5 (within 1 day) - Clinical signs:
- other: Dyspnoea, abnormal position, rattling, apathy, tumbling, atonia, spastic gait, tonic spasms, ruffled fur, cyanosis, dehydration, bulging eye, gummy eyes, abducted extremities, paresis, grave general condition. No clinical signs were observed in animals t
- Gross pathology:
- Animals which died during the observation period:
Heart: acute dilatation, acute congestive hyperaemia
Lung: acute swelling of moderate severity,
Stomach: extensive bloody ulceration, haemorrhagic corrosive gastritis
Gut: necrosis of the mucosa, bloody contents, severe congestion of the vessels
Animals sacrificed at the end of the observation period:
Organs without findings. - Other findings:
- No data.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Methoxyacetic acid is considered to be harmful following acute oral exposure.
- Executive summary:
In an acute oral toxicity study, groups of Sprague-Dawley rats (5/sex, males: 180 – 200 g, females: 160 – 170 g) were given a single oral dose of Methoxyacetic acid diluted in water at doses of 681, 1000, 1470, 2150, 3160 or 4640 mg/kg bw. Animals were then observed for 14 days. Clinical signs and dissection findings were registered.
Oral LD50 Males > 1000 < 1470 mg/kg bw
Oral LD50 Females = appr. 1000 mg/kg bw
Oral LD50 Combined > 1000 < 1470 mg/kg bw
Treatment related clinical signs were dyspnoea, abnormal position, rattling, apathy, tumbling, atonia, spastic gait, tonic spasms, ruffled fur,cyanosis, dehydration, bulging eye, gummy eyes, abducted extremities, paresis, grave general condition.
Animals that died during the observation period revealed following gross pathological changes: Heart: acute dilatation, acute congestive hyperaemia, lung: acute swelling of moderate severity, stomach: extensive bloody ulceration, haemorrhagic corrosive gastritis, Gut: necrosis of the mucosa, bloody contents, severe congestion of the vessels.
No macroscopic findings were observed in animals sacrificed at the end of the observation period.
This acute oral study is classified as acceptable. This study was performed prior to the implementation of GLP and OECD Guidelines, but is in compliance with the principles described in OECD Guideline 401.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.