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EC number: 422-210-5 | CAS number: 68957-94-8 T3P
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 95-07-10 till 95-07-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Adopted: Juli 17, 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Dated 07/31/1992
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Remarks:
- Not possible, because the composition of the test water disturbs the analysis by paramagnetic effects
- Vehicle:
- no
- Details on test solutions:
- No auxiliary agent was used.
The substance was diluted in water, homogenized with the ultra-turrax and given into test vessels. Afterwards the dilution was stirred for 60 min and the pH was adjusted to a pH of 8 with 10 m NaOH. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Brachydanio rerio
- Strain: Hamilton-Buchanan
- Source: Corporate Toxicology (Hoechst AG)
- Date of hatching: 10/07/95
- Length at study initiation: 3.2 - 3.5 cm, mean 3.3 cm
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test conditions
- Type and amount of food during acclimation: Tetra Min, Tetra Werke, Melle (FRG)
- Feeding frequency during acclimation: twice daily ad libitum
FEEDING DURING TEST
no feeding during test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 2.1 - 2.5 mmol Ca2+ + Mg2+/L
- Test temperature:
- 21 - 23 °C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 6.6 - 9.2 mg/L
- Nominal and measured concentrations:
- Nominal concentration: 0 and 100 mg/L
no measured concentrations - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass test chamber
- Material, size, fill volume: glass, length 30cm, width 22 cm, height 24 cm, 10 L volume
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1 g fish/L water
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to ISO/DIS 7346/1
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: In the 100 mg/L concentration vessel the pH was adjusted with 10 m NaOH
- Photoperiod: from 6 am to 6 pm
- Light intensity: 700 lux - Reference substance (positive control):
- not required
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Observations on body length and weight: no
- Mortality of control: no
- Abnormal responses: no - Validity criteria fulfilled:
- yes
- Conclusions:
- The test item was examined for acute toxicity to freshwater fish in a 96-h static test according to OECD Guideline 203 and EU method C.1. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.
- Executive summary:
The acute toxicity of the test item to fish (zebrafish) was determined according to the OECD Guideline 203 and EU method C.1.
A static test was performed as a limit test with a concentration of 100 mg/L. Seven test organisms were exposed to the test concentration and the control, respectively. Mortality, visible abnormalities and changes in behaviour were determined after 24, 48, 72 and 96 hours. The test substance undergoes fast hydrolysis in water with subsequent temperature and pH-dependent changes in the composition of the hydrolysis products. Analytical determination of the test compound or of the hydrolysis products in the test batches was not possible, because the composition of the test water disturbs the analysis by paramagnetic effects.
Test organisms exposed to 100 mg/L of the test item showed no changes in behaviour and appearance. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.
Reference
Description of key information
The test item was examined for acute toxicity to freshwater fish in a 96-h static test according to OECD Guideline 203 and EU method C.1. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item to fish (zebrafish) was determined according to the OECD Guideline 203 and EU method C.1.
A static test was performed as a limit test with a concentration of 100 mg/L. Seven test organisms were exposed to the test concentration and the control, respectively. Mortality, visible abnormalities and changes in behaviour were determined after 24, 48, 72 and 96 hours. The test substance undergoes fast hydrolysis in water with subsequent temperature and pH-dependent changes in the composition of the hydrolysis products. Analytical determination of the test compound or of the hydrolysis products in the test batches was not possible, because the composition of the test water disturbs the analysis by paramagnetic effects.
Test organisms exposed to 100 mg/L of the test item showed no changes in behaviour and appearance. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.
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