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EC number: 411-490-4 | CAS number: 7027-11-4 ISOPHORONENITRILE, IPN
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-10-21 to 1985-11-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- EC Number:
- 411-490-4
- EC Name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- Cas Number:
- 7027-11-4
- Molecular formula:
- C10H15NO
- IUPAC Name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- Details on test material:
- isophorone nitrile of Hüls AG, purity 99.3 %; impurity isophorone (78-59-1) 0.33 %
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ORGANISMS:
- Strain: Bor: WISW (SPF TNO)
- Source: F. Winkelmann, Borchen (Germany)
- Weight at study initiation: 5 males mean 121 g, 5 females mean 110 g
- Fasting period before study: 16 hours
- Diet: ad libitum, R10 special diet for rats, SSniff R
- Water: ad libitum, tab water
- Acclimation period: 4 - 8 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 1 °C
- Humidity (%): 60 +/- 5 %
- Photoperiod (hrs dark / hrs light): 12 hours artificial light, 12 hours dark
- Air changes (per hr): 15 per hour
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: corn oil
- Details on oral exposure:
- ADMINISTRATION:
- Preparation of test substance: melting at 80 °C and added to warm (50°C) corn oil
- Doses per time period: single dose (gavage)
- Volume administered or concentration: 10 ml/kg bw, 1.25-1.99 %
- Post dose observation period: 14 days - Doses:
- 125; 158; 199 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- EXAMINATIONS:
- Body weights: before, and 1, 7, 14 days post dosing
- Clinical signs and mortality: within 6 hours after dosing, thereafter daily
- Necropsy: all animals (macroscopic) - Statistics:
- LD50 is generally determined according to Litchfield and Wilcoxon, reported with 95 % confidence limits.
Means of body weights were calcultated
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 178 mg/kg bw
- 95% CL:
- 158 - 201
- Remarks on result:
- other: no remarks
- Mortality:
- MORTALITY:
- Number of deaths at each dose:
125 mg/kg bw: no deaths
158 mg/kg bw: 3 males, 1 female dead within 20 hours
199 mg/kg bw: 2 males, 4 females dead within 15 hours
- Clinical signs:
- CLINICAL SIGNS:
- 2-10 minutes after dosing: saltatory, rotatory, and extension spasms as well as tonic spasms with vocalization (in part), distinct opisthotonos
and exophthalmos, salivation, bleeding eyes and nose, ruffled fur
- later: prone or lateral position, gasping breathing or increased breathing rate, crouched posture and, from time to time, tremor
- 30-60 minutes after dosing: additionally slight sedation and ataxia in some animals
- 4-6 hours after dosing: ruffled fur and crouched posture frome time to time left in some animals
- 24 hours after dosing: no more signs of toxicity - Body weight:
- BODY WEIGHT: Body weight gain was not affected
- Gross pathology:
- NECROPSY FINDINGS:
- animals that died during the study: hyperemia of the gastric mucosa and reddish stomach contents in some animals, light flecks on liver
in 1 animal
- terminal sacrifice: No evidence of macroscopically discernible organ changes - Other findings:
- no other findings
Any other information on results incl. tables
no further remarks
Applicant's summary and conclusion
- Interpretation of results:
- toxic
- Remarks:
- Migrated information Criteria used for interpretation of results: other: EU Directive 67/548/EEC
- Conclusions:
- According to this study the LD50 value (oral) was determined to be 178 mg/kg bw in rats for the test item isophoronnitrile.
- Executive summary:
The test item Isophoronnitrile was applied once to 3 dose-groups of rats (5 male and 5 female Wistar rats per dose-group) in doses of 125, 158, 199 mg/kg bw as solution in corn oil. The observation period was 14 days. Animals died within 15 hours after oral application of the test item. Clinical signs were 2-10 minutes after dosing saltatory, rotatory, and extension spasms as well as tonic spasms with vocalization (in part), distinct opisthotonos and exophthalmos, salivation, bleeding eyes and nose, ruffled fur. Later they showed prone or lateral position, gasping breathing or increased breathing rate, crouched posture and, from time to time, tremor. After 24 hours all animals were free of signs of toxicity. Post mortem dessection revealed hyperaemia of the gastric mucosa and reddish stomach contents in some animals. Dissection at the end of the experiment revealed no evidence of mactoscopically descernible organ changes. According to this study the LD50 value (oral) was determined to be 178 mg/kg bw in rats for the test item isophoronnitrile.
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