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EC number: 806-984-5 | CAS number: 1392411-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 19, 2017 to February 9, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods'
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods'
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- Measurement of the O2-concentration
- Vehicle:
- no
- Details on test solutions:
- Due to the poor solubility of the test substance, the test substance was weighed directly into the test vessels.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used. The chosen plant treats mostly domestic sewage. Source: the sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant in D-67435 NW-Lachen-Speyerdorf. Pre-Treatment: Upon arrival in the test facility, the sludge was filtered, washed with tap water 3 times and re-suspended in tap water. The activated sludge was aerated until usage in the test and fed daily with 50 mL synthetic sewage feed /L. inoculum. The dry matter was determined as 3.2 g suspended solids/L, giving a concentration of 1.6 g suspended solids/L in the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 0.83 mmol/L
- Test temperature:
- 19.3 – 20.8°C (first experiment (pre-test))
19.1 – 20.5°C (second experiment (main test))
19.5 – 20.5°C (third experiment) - pH:
- 8.16
- Dissolved oxygen:
- Aeration: purified air, using Pasteur pipettes
- Salinity:
- sodium concentration: 4.7 mg/L
- Conductivity:
- 228 μS/cm (at 25°C)
- Nominal and measured concentrations:
- Nominal: 4 concentrations ranging from 1000 to 1 mg/L
- Details on test conditions:
- Replicates:
1 replicate/concentration (positive control, all experiments)
1 replicate/concentration test substance 100 / 10 / 1 mg/L (first experiment)
5 replicates/concentration test substance 1000 mg/L (first experiment)
5 replicates/concentration test substance (second and third experiment)
Blank Control 2 replicates before and 2 after measuring positive control and test substance, respectively.
On the day before each experiment, the inoculum was taken from its source, washed, aerated and the dry matter was determined. Volume was adapted to the desired content of dry matter. The nutrient solution was thawed and the sludge was fed with 50 mL nutrient solution / L sludge. On the day of the experiment, the dry matter was determined once more. The stock solution of the positive control was prepared.
In the blank control vessels, 16 mL nutrient solution was mixed with 234 mL water. The positive control vessels and the treatments were prepared by putting the appropriate amount of positive control solution respectively test substance into the respective test vessel, adding 16 mL nutrient solution and water to reach a total volume of 250 mL. Then, 250 mL inoculum was added in 5 min intervals and the mixtures were aerated. After 3 h of incubation, the content of the first vessel was poured in a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of max. 5 min. The following vessels were measured likewise in 5 min intervals. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 360 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- 3,5-Dichlorophenol (CAS No. 591-35-5) was used as positive control. Stock solutions in deionised water containing 500 mg/L (first experiment), 501 mg/L (second experiment) and 500 mg/L (third experiment) were freshly prepared for each experiment.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 3 h EC50 and NOEC of the substance to sludge microorganisms were determined to be >1000 mg/L and 22 mg/L (nominal) respectively.
- Executive summary:
A study was conducted to determine the toxicity of the substance on the respiration of sludge microorganisms according to OECD Guideline 209 and EU Method C.11, in compliance with GLP. The dry matter of the inoculum was determined to be 3.2 g suspended solids/L, giving a concentration of 1.6 g suspended solids/L in the test. The sludge was exposed to the test substance at concentrations ranging from 1 to 1000 mg/L for 3 h. Due to the poor solubility of the test substance in water, the test substance was weighed directly into the test vessels to which sludge was added later. Inhibition of respiration at the lowest treatment concentration was significant in the two initial tests, and a third experiment was performed to determine the NOEC at lower concentrations. In the third experiment the test substance was tested using 3 concentrations ranging from 22 to 4.6 mg/L (nominal concentration). Under the study conditions, the 3 h EC50 and NOEC of the substance to sludge microorganisms were determined to be >1000 mg/L and 22 mg/L (nominal) respectively (Muckle, 2017).
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 22 mg/L
Additional information
A study was conducted to determine the toxicity of the substance on the respiration of sludge microorganisms according to OECD Guideline 209 and EU Method C.11, in compliance with GLP. The dry matter of the inoculum was determined to be 3.2 g suspended solids/L, giving a concentration of 1.6 g suspended solids/L in the test. The sludge was exposed to the test substance at concentrations ranging from 1 to 1000 mg/L for 3 h. Due to the poor solubility of the test substance in water, the test substance was weighed directly into the test vessels to which sludge was added later. Inhibition of respiration at the lowest treatment concentration was significant in the two initial tests, and a third experiment was performed to determine the NOEC at lower concentrations. In the third experiment the test substance was tested using 3 concentrations ranging from 22 to 4.6 mg/L (nominal concentration). Under the study conditions, the 3 h EC50 and NOEC of the substance to sludge microorganisms were determined to be >1000 mg/L and 22 mg/L (nominal) respectively (Muckle, 2017).
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