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EC number: 252-512-0 | CAS number: 35325-02-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08/08/2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg CaCO3 /L
- Dissolved oxygen:
- no data
- Nominal and measured concentrations:
- 100,10000 mg/L Nominal
measured 88.57 and 9543.58 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: /
- Aeration: /
- Type of flow-through (e.g. peristaltic or proportional diluter): /
- Renewal rate of test solution (frequency/flow rate):/
- Initial cells density: 1*10E4 cells/ml
- Control end cells density: at the beginning of the test, cells were counted by microscope, using a counting chamber. Thereafter cell densities were determined by spectrophotometric measurement of samples at 720 nm using a Varian Cary 50 single beam spectrophotometer with immersion probe.
- No. of organisms per vessel:/
- No. of vessels per concentration (replicates):/
- No. of vessels per control (replicates):/
- No. of vessels per vehicle control (replicates):/
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:tap water purified by reverse osmosis and then passed over activated carbon and ion-exchange cartridges.
- Total organic carbon:/
- Particulate matter:/
- Metals:/
- Pesticides:/
- Chlorine:/
- Alkalinity:/
- Ca/mg ratio: hardness: 0.24 mmol/l
- Conductivity:/
- Culture medium different from test medium:/
- Intervals of water quality measurement: /
OTHER TEST CONDITIONS
- Sterile test conditions: yes/
- Adjustment of pH:/
- Photoperiod:continuously
- Light intensity and quality: TLD-lamps of the type 'cool-white' of 30 watt (Sylvania), with a light intensity within the range of 60 to 120 µE.m-2.s-1, not varying by more than 202%.
- Salinity (for marine algae):/
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber
- Chlorophyll measurement:/
- Other: NH4Cl: 15 mg/l
MgCl2.6H2O: 12 mg/l
CaCl2.2H2O: 18 mg/l
MgSO4.7H2O: 15 mg/l
KH2PO4: 1,6 mg/l
FeCl3.6H2O: 80 µg/l
Na2EDTA.2H2O: 100 µg/l
MnCl2.4H2O: 415 µg/l
ZnCl2: 3µg/l
CoCl2.6H2O: 1,5 µg/l
CuCl2.2H2O: 0,01 µg/l
Na2MoO4.2H2O: 7µg/l
NaHCO3: 50 mg/l
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline: /
- Range finding study
- Test concentrations: a range of 0,1 to 100 mg/l, increasing by a factor 10.
- Results used to determine the conditions for the definitive study:based on the result of the range-finding test the EC50 for effects on algal growth was expected to lie between 10 and 100 mg/l.
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2 340 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Unusual cell shape:
- Colour differences:
- Flocculation:
- Adherence to test vessels:
- Aggregation of algal cells:
- Other:
- Any stimulation of growth found in any treatment:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium:no - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for cell growth inhibition (EC50: 0-72h) was 2340 mg/l
As the concentrations of N-(hydroxypropyl)benzene sulphonamide measured by HPLC remains within 80-120 of the nominal concentration, the result are based on nominal concentrations
Reference
Description of key information
The result of a range finding study was >1000 mg/kg
The 72 h Algae Pseudokirchneriella subcapitata EC 50 =2340 mg/kg
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2 340 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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