2-[N-(2-cyanoethyl)-4-[(2,6-dichloro-4-nitrophenyl)azo]anilino]ethyl acetate

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From December 3, 2007 to March 12, 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)

GLP compliance:

not specified

Details on sampling:

Samples of solutions and test organisms from both the control and test solutions were taken daily from the 26th day to the last day during the exposure test period.

Vehicle:

yes

Remarks:

dechlorinated drinking water

Details on preparation of test solutions, spiked fish food or sediment:

Dechlorinated drinking water was used in the test. Waters with total hardness of between 10 and 250 mg CaCO3 per liter, and with a pH 6.0 to 8.5 are preferable.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Test organisms were obtained from Amazonas Commercial Aquarium, Shanghai.

Prior to the test start, fish were acclimated for one week under conditions of water quality and illumination similar to those used in the test. During holding and acclimatization until one day before the start of the test, the fish were fed with a commercial fish diet. Fish were fed 2 h before water renewal during the test to keep their lipid content unaltered.

From the acclimated test fish batch, 10 fish were measured at the start of the test. The mean body length of the fish was 3.91±0.18 cm (Mean ± SD), and the mean body weight was 0.32±0.06 g (Mean ± SD).

During holding and acclimatization no fish died in the test fish batch and all fish were healthy.

Route of exposure:

aqueous

Test type:

semi-static

Water / sediment media type:

other: Dechlorinated drinking water

Total exposure / uptake duration:

28 d

Hardness:

between 10 and 250 mg CaCO3 per liter

Test temperature:

23.1 +/- 1°C

pH:

between 7.22 and 8.01

Dissolved oxygen:

>80%

Nominal and measured concentrations:

20 mg/L

Lipid content:

> 0.57 - < 1.25 %

Time point:

other: From Day 26 to 28 of exposure

Key result

Conc. / dose:

20 mg/L

Temp.:

23.1 °C

Type:

BCF

Value:

< 100 dimensionless

Basis:

other: ratio of the concentration of the test substance in the fish and in water

Calculation basis:

steady state

- In the controls and at 20 mg/L (mean measured concentration 0.028~0.28 mg/L) no fish died or other adverse effects was observed during the exposure period of 28 d.

- All of the analytically measured test substance concentrations in the exposure test medium (C_w) were 0.028~0.28 mg/L from the first day to the third day during the sampling period. However, all of the analytically measured test substance concentrations in the extracted solution were <0.028 mg/L from the first day to the third day of sampling. Based on the measured concentrations in the test medium and the test organism, the bioconcentration factor (BCF) of the test substance was calculated to be <100.

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditons, bioconcentration factor of the test substance was determined to be <100.

Executive summary:

A study was conducted to determine the bioaccumulation of the test substance in fish according to OECD Guideline 305 B. Danio rerio were exposed to the test substance at a concentration of 20 mg/L for 28 d. The sampling and measurement of lipid content of the fish to determine bioconcentration were performed daily from Days 26 to 28. The sampled fish were rinsed quickly with water, killed with a pin as soon as possible and weighed. The lipid component of sampled fish were then extracted and weighed. Samples of the test solution were analyzed for test substance concentration. The measured concentration of the test substance in water was 0.028-0.28 mg/L. The lipid content in the sampled fish was between <0.66 and <0.81 mg/kg. Under the study conditions, the bioconcentration factor (BCF) of the test substance was determined to be <100 (Shen, 2008).

Key value for chemical safety assessment

BCF (aquatic species):

100 dimensionless

Additional information

A study was conducted to determine the bioaccumulation of the test substance in fish according to OECD Guideline 305 B. Danio rerio were exposed to the test substance at a concentration of 20 mg/L for 28 d. The sampling and measurement of lipid content of the fish to determine bioconcentration were performed daily from Days 26 to 28. The sampled fish were rinsed quickly with water, killed with a pin as soon as possible and weighed. The lipid component of sampled fish were then extracted and weighed. Samples of the test solution were analyzed for test substance concentration. The measured concentration of the test substance in water was 0.028-0.28 mg/L. The lipid content in the sampled fish was between <0.66 and <0.81 mg/kg. Under the study conditions, the bioconcentration factor (BCF) of the test substance was determined to be <100 (Shen, 2008).