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EC number: 258-649-2 | CAS number: 53585-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 1993-06-08 to 1993-07-06
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- other: BBA-Richtlinie BBA-CP 411 3/90
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
- Principles of method if other than guideline:
- Examination of the reproductive performance of Collembolen for the NOEC value determination after 28 days.
- GLP compliance:
- yes (incl. QA statement)
- Application method:
- soil
- Specific details on test material used for the study:
- The purity of the material used for the test was lower than the purity of the actual material. It contained ca. 26% of isomers of dimethyl tribenzenes. However, based on comparable basic structure of the impurities and the assumption that any toxic effects would increase with higher molecular weight and lipophilicity, we regard that the studies conducted with the lower purity material being "worst case" and can be used for the evaluation of the actual product.
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- TEST BATCH AND ITS PREPARATION
- Vehicle, solvent: no vehicle/solvent used
- Procedure: 1 application at the beginning of the test, the test substance was weighed in silica sand and then mixed with the other soil components. The soil substrate was distributed to the test vessels and the test organisms were set in immediately after. - Test organisms (species):
- Folsomia candida
- Animal group:
- Collembola (soil-dwelling springtail)
- Details on test organisms:
- TEST ORGANISMS
- Strain: Folsomia candida (Willem)
- Supplier: Battelle Europe e.V., Frankfurt a.M., Germany
- Wild caught: no
- Breeding method: at 20-22°C and diffuse light (approx. 2.2 µmol/m²s, natural diurnal rhythm) in transparent round polyethylene vessels (7 cm diameter, 7.5 cm height), culture substrate consisting of 8 parts gypsum and 1 part charcoal (40 % moisture), feeding 1 ¿ 2 times per week, approx. 10 mg grained dry yeast (Dr. Oetker, Bielefeld, Germany) per breeding container
- Age: 10 ¿ 12 days old
- Substrate: 69 % silica sand, 20 % kaoline, 10 % turf, 1 % lime, moistened with aerated, dechlorinated tap water up to a moisture of approx. 25 %
- Feeding: 2 mg grained dry yeast each attempt at the beginning of the test and after 14 days
- Pretreatment: none - Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 28 d
- Post exposure observation period:
- two times per week.
- Test temperature:
- 18 - 25 °C
- pH (if soil or dung study):
- 7.29 (1000 mg/kg dw) / 7.46 (control) at the beginning of the test;
7.91 (control) / 7.95 (100 mg/kg dw), resp. 7.93 (1000 mg/kg dw) at the end of the test
- Adjustment of pH: not adjusted - Humidity:
- approx. 25 %
- Photoperiod and lighting:
- not mentioned
- Details on test conditions:
- - Exposure vessel type: 250 ml glass flasks with screw top, 2 times per week the test vessels were opened 2 minutes for ventilation
- Number of replicates, collembola per replicate: 4 replicates, 10 collembola per replicate, 1 replicate per concentration without collembola for determination of soil parameter - Nominal and measured concentrations:
- Nominal: 0 (control), 10, 32, 100, 320 and 1000 mg/kg dry weight
- Reference substance (positive control):
- no
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 320 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- RESULTS: EXPOSED
- Nominal concentrations: 10, 32, 100, 320, 1000 mg/kg dw
- Effect data:
Mortality of adults after 28 days (mean values):
10 mg/kg / 22.5 %
32 mg/kg / 12.5 %
100 mg/kg / 22.5 %
320 mg/kg / 55* %
1000 mg/kg / 63.5* %
Number of juveniles after 28 days (mean values):
10 mg/kg / 726
32 mg/kg / 785
100 mg/kg / 752
320 mg/kg / 631
1000 mg/kg / 424*
* significant deviation (DUNNET¿s-test)
RESULTS: CONTROL
- Effect data:
Mortality: 12.5 %
Number of juveniles: 726
RESULTS: TEST WITH REFERENCE SUBSTANCE
No reference substance tested - Results with reference substance (positive control):
- not performed
- Reported statistics and error estimates:
- statistics procedure according DUNNET with program SigmaStat 5.0.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The mortality of Folsomia in the test groups with 320 mg/kg and 1000 mg/kg dry soil was significantly above the control groups. In the highest test concentration the mortality was 62.5 %. In the highest concentration only dead juveniles was found. No effects on reproduction were found in the 320 mg/kg dry soil test group.
mortality: NOEC = 100 mg/kg dry soil, LOEC = 320 mg/kg dry soil
reprodution: NOEC = 320 mg/kg dry soil, LOEC = 1000 mg/kg dry soil - Executive summary:
The mortality of in the test groups with 320 mg/kg and 1000 mg/kg dry soil was significantly above the control groups. In the highest test concentration the mortality was 62.5 %. In the highest concentration only dead juveniles was found. No effects on reproduction were found in the 320 mg/kg dry soil test group.
mortality: NOEC = 100 mg/kg dry soil, LOEC = 320 mg/kg dry soil
reprodution: NOEC = 320 mg/kg dry soil, LOEC = 1000 mg/kg dry soil
Reference
Description of key information
The mortality of the collemboles in the test groups with 320 mg/kg and 1000 mg/kg dry soil was significantly above the control groups. In the highest test concentration, the mortality was 62.5 %. In the highest concentration only dead juveniles was found. No effects on reproduction were found in the 320 mg/kg dry soil test group.
The NOEC value for mortality was determined to be equal to 100 mg/kg dry soil (LOEC = 320 mg/kg dry soil)
The NOEC value for reproduction was determined to be equal to 320 mg/kg dry soil (LOEC = 1000 mg/kg dry soil)
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil dwelling arthropods:
- 100 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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