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EC number: 265-747-9 | CAS number: 65405-84-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-09-14 to 2015-11-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- α,2,6,6-tetramethyl-2-cyclohexene-1-butyraldehyde
- EC Number:
- 277-458-5
- EC Name:
- α,2,6,6-tetramethyl-2-cyclohexene-1-butyraldehyde
- Cas Number:
- 73398-85-3
- Molecular formula:
- C14H24O
- IUPAC Name:
- 2-methyl-4-(2,6,6-trimethylcyclohex-2-en-1-yl)butanal
- Reference substance name:
- α,2,6,6-tetramethylcyclohexene-1-butyraldehyde
- EC Number:
- 282-993-2
- EC Name:
- α,2,6,6-tetramethylcyclohexene-1-butyraldehyde
- Cas Number:
- 84518-22-9
- Molecular formula:
- C14H24O
- IUPAC Name:
- 2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)butanal
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Test material name (as stated in the report): Cetonal
Batch No.:SC00011028
Expiry date: 23 June, 2016
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure intervals (0 and 24 hours), sampling was carried out after preparation of the test concentrations.
At the end of the exposure intervals (24 and 48 hours), samples were taken directly from the test vessels.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The test item was expected to evaporate. To reduce losses of the test item, the study was conducted in a closed system without headspace.
A stock solution of 3.00 mg/L of the test item was prepared one day before the start of each exposure interval (at -24 and 0 hours). For this purpose, 6.75 pL of the test item (density of the test item taken into account) were directly introduced by pipette into a 2 L glass flask, completely filled with the dilution water (approximately 2065 mL of ISO test water as specified below) below the water surface while stirring.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test system: Daphnia magna STRAUS (Clone 5)
Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
20 daphnids, divided into 4 replicates, each with 5 daphnids, were used per concentration level and control.
Age of the Daphnia: 2 to 24 hours old daphnids from a healthy stock were used for the study. Juvenile daphnids were removed from the culture vessels latest 22 hours before the start of the exposure and discarded. The juveniles born within this period of max. 22 hours preceding the exposure were used for the test after an acclimatisation phase in the dilution water of at least 2 hours. No first brood progeny was used for the test.
Acclimatisation: At least 2 hours in dilution water
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 262 to 267 fmg CaCCb/Ll
- Test temperature:
- 20.4 to 20.3 °C
- pH:
- 7.96 to 7.75
- Dissolved oxygen:
- 7.03 to 8.28 [mg/L]
- Conductivity:
- 653 to 648 microS/cml
- Nominal and measured concentrations:
- 5 test item concentrations in a geometric series with a separation factor of 2, prepared by diluting the stock solution of 3.00 mg/L with dilution water, were tested as follows:
0.188 - 0.375 - 0.750 - 1.50 - 3.00 mg/L.
The nominal concentrations mentioned above correspond to the geometric mean measured concentrations of:
0.156 - 0.321 - 0.676 - 1.51 - 2.99 mg/L. - Details on test conditions:
- The study was conducted in a closed system (sealed glass flasks) without headspace under semistatic conditions over a period of 48 hours with 5 concentrations in the range of 0.188 to 3.00 mg/L of the test item CETONAL in a geometric series with a separation factor of 2.
The test item CETONAL is a pale yellow liquid with a water solubility of 4.63 mg/L (20 °C, pH 7). The test item was expected to evaporate. To reduce losses of the test item, the study was conducted in a closed system without headspace. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a. (Sigma)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.739 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The measured concentrations of the test item at the start of the exposure intervals (0 and 24 hours) were in the range of 93 to 124 % of the nominal concentrations. At the end of the exposure intervals (24 and 48 hours), the measured concentrations of the test item were in the range of 65 to 92 % of the nominal concentrations.
Since the measured test item concentrations did not remain within ± 20 % of the nominal concentrations, the effect values given in Table 1 are based on the geometric mean measured concentrations of the test item CETONAL calculated as follows:
0.156 - 0.321 - 0.676 - 1.51 - 2.99 mg/L. - Results with reference substance (positive control):
- A reference test is conducted by the laboratory under static conditions with a test duration of 24 hours once per month in order to prove the validity of the test system and test conditions at the test facility. The EC50-value for the most recent of the monthly performed reference tests was 1.61 mg/L (Cl: 1.43 - 1.82 mg/L).
The EC50-value of the reference item potassium dichromate after 24 hours is within the prescribed concentration range of 0.6 - 2.4 mg/L of quality criteria according to AQS P 9/2 (02/2000) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.
Any other information on results incl. tables
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Acccording to the test conditions the EC50(48hours) of the test item CETONAL with 95 % confidence limits has been determined to be 0.739 [mg/L] (Cl: 0.707 - 0.774) based on geometric mean measured test item concentrations.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item CETONAL (batch number: SC00011028) were determined according to OECD 202 guideline.
Twenty daphnids were exposed to each concentration level and the control. The concentrations of the test item CETONAL were analytically verified after derivatisation using 2,4-Dinitrophenylhydrazine via LC-MS/MS at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours) in all concentration levels and the control. The measured concentrations of the test item at the start of the exposure intervals (0 and 24 hours) were in the range of 93 to 124 % of the nominal concentrations. At the end of the exposure intervals (24 and 48 hours), the measured concentrations of the test item were in the range of 65 to 92 % of the nominal concentrations.
Since the measured test item concentrations did not remain within ± 20 % of the nominal concentrations, the effect values given in Table 1 are based on the geometric mean measured concentrations of the test item CETONAL calculated as follows: 0.156 - 0.321 - 0.676 - 1.51 - 2.99 mg/L.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.
The EC50(48hours) with 95 % confidence limits has been determined to be 0.739 [mg/L] (Cl: 0.707 - 0.774) based on geometric mean measured test item concentrations.
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