Isobutyl (S)-2-chloropropionate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983-08-23 to 1983-09-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable with guideline study (non-GLP).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Principles of method if other than guideline:

BASF-Test, principally according to OECD guideline 403.

GLP compliance:

no

Test type:

fixed concentration procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyl (S)-2-chloropropionate

EC Number:

280-349-5

EC Name:

Isobutyl (S)-2-chloropropionate

Cas Number:

83261-15-8

Molecular formula:

C7H13ClO2

IUPAC Name:

isobutyl (S)-2-chloropropanoate

Details on test material:

- Name of test material (as cited in study report): L-chloropropionic acid isobutyl ester
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Lot/batch No.: P 4/83
No further data.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
male and female SPF Wistar /Chbb:Thom rats
- Source: Dr . K . Thomae GmbH, 7950 Biberach, Germany
- Age at study initiation: 8 weeks
- Weight at study initiation: mean weight: 240 g (males), 168 g (females)
- Fasting period before study: no data
- Housing: 3 per cage, steel cages (Becker, type D III)
- Diet (ad libitum): KLIBA Labordiät Ratte/Maus A 343 10 mm Pellet, Klingentalmühle AG, CH-4303 Kaiseraugst (Switzerland)
- Water: tap water, ca. 250 ml per cage/day
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: no data

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
A 5-cm layer of the test material was filled into a fritted glass disc (diameter 30 cm) and weighed. An air stream (200 l/h) was bubbled through this substance layer at a temperature of 19-21°C. The so generated substance-air mixture was conducted through 6 glass tubes. In these tubes, 6 rats (3 males, 3 females) had been placed. Exposure temperature was 19-25 °C. Exhausted air/mixture was discarded.
The fritted glass containing the test substance was replaced after 30 minutes. The new substance was used for the remainder of the exposure period. The loss of test material was determined by re-weighing the residual test material in the fritted glasses.
Total exposure time was 7 hours. The aim of the study was to determine the time of exposure which was survived by all animals after a 14-day observation in two experiment.

Analytical verification of test atmosphere concentrations:

no

Remarks:

calculation of nominal concentration

Duration of exposure:

7 h

Concentrations:

8.1 mg/l (nominal); saturated atmosphere

No. of animals per sex per dose:

3 males, 3 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: on working days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology

Statistics:

no data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: IHT

Effect level:

8.1 mg/L air (nominal)

Exp. duration:

7 h

Remarks on result:

other: no mortality after exposure for 7 h to a saturated vapour atmosphere.

Mortality:

No deaths occurred.

Clinical signs:

other: During exposure, eyelid closure, serous secretion of eyes and nose (hemotest positive), salivation, and intermittent respiration was noted. After exposure, intermittent respiration and apathy was observed. The animals were normal after one day.

Body weight:

no data

Gross pathology:

No pathological findings were observed.

Any other information on results incl. tables

The mean concentration of the test substance was estimated to be 8.1 mg/l (nominal).

Applicant's summary and conclusion

Executive summary:

No mortality was observed when 6 rats were exposed for 7 hours to an atmosphere that had been saturated at 20° with the volatile parts of the compound. The mean concentration of the test substance was 8.1 mg/l (nominal).