3-methylbut-2-en-1-ol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test method comparable to guideline study, in accordance with accepted German national standards.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412, part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-2-en-1-ol

EC Number:

209-141-4

EC Name:

3-methylbut-2-en-1-ol

Cas Number:

556-82-1

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-2-en-1-ol

Details on test material:

- Name of test material (as cited in study report): 3-Methylbuten-2-ol-1 (Prenol)
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Stock solution with 1 g/l:  

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

21 - 25 °C

pH:

pH at test start: 8.0

Nominal and measured concentrations:

Nominal test concentrations: 31.25, 62.5, 125, 250, 500 mg/l and a control

Details on test conditions:

TEST SYSTEM
- Test vessel: reagent tube with flat bottom (volume 10 ml)
- Fill volume: 8 ml
- Initial cells density: 1000
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5


OTHER TEST CONDITIONS
- Photoperiod: artificial light, permanent
- Light intensity and quality: 120 uE/m2s


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Determination of cell density after 0, 24, 48, 72, and 96 h  (fluorescence at 685 nm)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

213 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Any other information on results incl. tables

RS-Freetext:
Results after 96 h were as follows:

EbC10  = 225 mg/l
EbC50  = > 500 mg/l
EbC90  = > 500 mg/l

Volatility screening test:
Based on TOC measurements the recovery rate at the end of the exposure  period was 98 % for the algae test.
This shows that the test substance concentration remained nearly constant  under the conditions of the eco-toxicity test and therefore the effect  values based on nominal concentrations can be used for the hazard  assessment without limitations.